G2C::Proteomics

Targeted tandem affinity purification of PSD-95 recovers core
postsynaptic complexes and schizophrenia susceptibility proteins
Esperanza Fernandez, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama,
Mike DR Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary and Seth GN Grant


Supplementary Table 2. Proteins identified by LC-MS/MS in three tandem purifications from wild type mice.

Gene symbols, UniProt and IPI accession numbers and number of approved peptides for each protein identified by
LC-MS/MS analysis in the 4 tandem purifications from PSD-95TAP/TAP mice (T1, T2, T3, T4) and 3 tandem
purifications from wt mice (WT1, WT2, WT3) are shown.

MGI symbolProtein AccIPI AccDescription
Number of peptides
T1
T2
T3
T4
WT1
WT2
WT3
Atp1a2Q6ZQ49IPI00762871IPI:IPI00762871.2|TREMBL:Q6ZQ49|ENSEMBL:ENSMUSP00000095072|VEGA:OTTMUSP00000024454 Tax_Id=10090 Gene_Symbol=Atp1a2 MKIAA0778 protein
4
4
8
7
4
2
3
Hspa2P17156IPI00331546IPI:IPI00331546.2|SWISS-PROT:P17156 Tax_Id=10090 Gene_Symbol=Hspa2 Heat shock-related 70 kDa protein 2
7
7
7
9
3
1
1
Glud1XP_992848IPI00753095"IPI:IPI00753095.1|REFSEQ:XP_992848 Tax_Id=10090 Gene_Symbol=Glud1 similar to Glutamate dehydrogenase 1, mitochondrial precursor (GDH) isoform 2"
8
3
7
7
6
3
4
Atp1a3Q6PIC6IPI00122048IPI:IPI00122048.2|SWISS-PROT:Q6PIC6|TREMBL:Q8CGD9;Q8R0B0;Q8R0E8|VEGA:OTTMUSP00000017754 Tax_Id=10090 Gene_Symbol=Atp1a3 Sodium/potassium-transporting ATPase alpha-3 chain
9
8
11
9
6
2
4
AldoaP05064IPI00221402IPI:IPI00221402.7|SWISS-PROT:P05064|TREMBL:Q5FWB7;Q6NY00|ENSEMBL:ENSMUSP00000032934|REFSEQ:NP_031464 Tax_Id=10090 Gene_Symbol=Aldoa Fructose-bisphosphate aldolase A
13
7
10
11
6
3
Hspa8P63017IPI00323357IPI:IPI00323357.3|SWISS-PROT:P63017|TREMBL:Q3KQJ4;Q3TB63;Q3TEK2;Q3TF16;Q3TH04;Q3TH56;Q3TQ13;Q3TRH3;Q3TZJ3;Q3U9L2;Q3UBA6;Q3UBR0;Q3UDS0;Q3ULM1;Q3UXU1;Q504P4;Q9QZ38|ENSEMBL:ENSMUSP00000015800|REFSEQ:NP_112442 Tax_Id=10090 Gene_Symbol=Hspa8 Heat shock cognate
21
22
19
23
5
2
3
Atp1a1Q8VDN2IPI00311682IPI:IPI00311682.5|SWISS-PROT:Q8VDN2|TREMBL:Q3TXF9;Q3UCH8;Q3UF49;Q8K2R2;Q8K2Y5;Q8R3M4|ENSEMBL:ENSMUSP00000039657;ENSMUSP00000062842|REFSEQ:NP_659149|VEGA:OTTMUSP00000017686 Tax_Id=10090 Gene_Symbol=Atp1a1 Sodium/potassium-transporting ATPase alpha-1 chain
6
4
7
6
4
1
3
-ENSMUSP00000097709IPI00761732IPI:IPI00761732.1|ENSEMBL:ENSMUSP00000097709 Tax_Id=10090 Gene_Symbol=- 49 kDa protein
2
2
8
3
3
1
1
Eef1a1P10126IPI00307837IPI:IPI00307837.5|SWISS-PROT:P10126|TREMBL:Q3TII3;Q3UA81;Q3UZQ3;Q4FZE5;Q58E64|ENSEMBL:ENSMUSP00000042457|REFSEQ:NP_034236|VEGA:OTTMUSP00000021291 Tax_Id=10090 Gene_Symbol=Eef1a1 Elongation factor 1-alpha 1
2
2
7
4
3
1
1
Eef1a2P62631IPI00119667IPI:IPI00119667.1|SWISS-PROT:P62631|ENSEMBL:ENSMUSP00000054556|REFSEQ:NP_031932|VEGA:OTTMUSP00000017863 Tax_Id=10090 Gene_Symbol=Eef1a2 Elongation factor 1-alpha 2
2
2
7
3
3
1
1
Crmp1P97427IPI00312527IPI:IPI00312527.4|SWISS-PROT:P97427|TREMBL:Q3TXY0;Q6P1J1|ENSEMBL:ENSMUSP00000031004|REFSEQ:NP_031791 Tax_Id=10090 Gene_Symbol=Crmp1 Crmp1 protein
3
1
8
3
RpsaP14206IPI00123604IPI:IPI00123604.4|SWISS-PROT:P14206|ENSEMBL:ENSMUSP00000035105|REFSEQ:NP_035159 Tax_Id=10090 Gene_Symbol=Rpsa 40S ribosomal protein SA
3
Atp1a4Q9WV27IPI00378485IPI:IPI00378485.2|SWISS-PROT:Q9WV27|TREMBL:Q91ZN9|ENSEMBL:ENSMUSP00000007346|VEGA:OTTMUSP00000024456 Tax_Id=10090 Gene_Symbol=Atp1a4 Sodium/potassium-transporting ATPase alpha-4 chain
2
2
1
1
CkbQ04447IPI00136703IPI:IPI00136703.1|SWISS-PROT:Q04447|ENSEMBL:ENSMUSP00000001304|REFSEQ:NP_067248 Tax_Id=10090 Gene_Symbol=Ckb Creatine kinase B-type
3
2
3
4
1
2
3
PrkcbP68404IPI00227898IPI:IPI00227898.3|SWISS-PROT:P68404|TREMBL:A0JNZ5|ENSEMBL:ENSMUSP00000070019|REFSEQ:NP_032881|VEGA:OTTMUSP00000017803 Tax_Id=10090 Gene_Symbol=Prkcb1 Protein kinase C beta type
3
2
3
4
5
Cap2Q9CYT6IPI00112001IPI:IPI00112001.1|SWISS-PROT:Q9CYT6|TREMBL:Q8BKZ3|ENSEMBL:ENSMUSP00000021802|REFSEQ:NP_080332 Tax_Id=10090 Gene_Symbol=Cap2 Adenylyl cyclase-associated protein 2
1
1
7
Ppm1bP36993-5IPI00222989IPI:IPI00222989.1|SWISS-PROT:P36993-5 Tax_Id=10090 Gene_Symbol=Ppm1b Isoform Beta-5 of Protein phosphatase 1B
1
2
1
4
Pkm2P52480-2IPI00845840IPI:IPI00845840.1|SWISS-PROT:P52480-2 Tax_Id=10090 Gene_Symbol=Pkm2 Isoform M1 of Pyruvate kinase isozymes M1/M2
7
6
11
12
1
2
-Q52L64IPI00785509IPI:IPI00785509.1|TREMBL:Q52L64|ENSEMBL:ENSMUSP00000100179 Tax_Id=10090 Gene_Symbol=- Hypothetical protein
1
4
4
PrkcaQ3TQ39IPI00652220"IPI:IPI00652220.1|TREMBL:Q3TQ39|ENSEMBL:ENSMUSP00000097875|VEGA:OTTMUSP00000017462 Tax_Id=10090 Gene_Symbol=Prkca 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230094C20 product:similar to Protein kinase C beta 2"
2
3