G2C::Electrophysiology

NR2A-2BCTR knock-in mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with only one variable, burst 8-10 ave amplitude vs burst 2-4, significantly increased in these mutants. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the NR2A-2BCTR knock-in line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 31 (9) 3146 (91) 24 (7) 3183 (135) 0.53 0.81
PPF at 50ms, ampl ratio % 31 (9) 181.4 (3.6) 24 (7) 181.4 (3.8) 0.22 0.99
Burst 1, PPF at 10ms, ampl ratio % 31 (9) 189 (5.7) 24 (7) 172.8 (8) 0.091 0.18
Burst 1, EPSP3 depr, ampl ratio % 31 (9) 62.5 (1.9) 24 (7) 64.8 (2.3) 0.52 0.44
Burst 1, ave amplitude µV 31 (9) 1319 (32) 24 (7) 1398 (53) 0.41 0.22
Burst 2, ave amplitude µV 31 (9) 2191 (63) 24 (7) 2322 (97) 0.36 0.28
Burst 3, ave amplitude µV 31 (9) 2424 (68) 24 (7) 2553 (101) 0.45 0.3
Burst 4, ave amplitude µV 31 (9) 2478 (68) 24 (7) 2605 (93) 0.42 0.29
Burst 5, ave amplitude µV 31 (9) 2439 (63) 24 (7) 2540 (86) 0.45 0.35
Burst 6, ave amplitude µV 31 (9) 2373 (62) 24 (7) 2443 (78) 0.44 0.49
Burst 7, ave amplitude µV 31 (9) 2297 (59) 24 (7) 2342 (74) 0.49 0.63
Burst 8, ave amplitude µV 31 (9) 2226 (58) 24 (7) 2255 (72) 0.4 0.77
Burst 9, ave amplitude µV 31 (9) 2164 (59) 24 (7) 2178 (70) 0.39 0.89
Burst 10, ave amplitude µV 31 (9) 2123 (57) 24 (7) 2128 (69) 0.42 0.95
Burst 2-4 ave amplitude vs Burst 1 % 31 (9) 180.1 (3.9) 24 (7) 178.6 (3.1) 0.46 0.79
Burst 8-10 ave amplitude vs Burst 2-4 % 31 (9) 92 (0.9) 24 (7) 88.4 (0.9) 0.25 0.024 *
LTP vs PTP, amplitude ratio % 31 (9) 80.8 (4) 24 (7) 77.9 (0.9) 0.19 0.59
LTP based on amplitude % 31 (9) 174.9 (3.9) 24 (7) 166 (3.8) 0.83 0.075

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 31 (9) 3146 (91) 24 (7) 3183 (135) 0.53 0.81

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 31 (9) 181.4 (3.6) 24 (7) 181.4 (3.8) 0.22 0.99

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 31 (9) 189 (5.7) 24 (7) 172.8 (8) 0.091 0.18
Burst 1, EPSP3 depr, ampl ratio % 31 (9) 62.5 (1.9) 24 (7) 64.8 (2.3) 0.52 0.44
Burst 1, ave amplitude µV 31 (9) 1319 (32) 24 (7) 1398 (53) 0.41 0.22
Burst 2, ave amplitude µV 31 (9) 2191 (63) 24 (7) 2322 (97) 0.36 0.28
Burst 3, ave amplitude µV 31 (9) 2424 (68) 24 (7) 2553 (101) 0.45 0.3
Burst 4, ave amplitude µV 31 (9) 2478 (68) 24 (7) 2605 (93) 0.42 0.29
Burst 5, ave amplitude µV 31 (9) 2439 (63) 24 (7) 2540 (86) 0.45 0.35
Burst 6, ave amplitude µV 31 (9) 2373 (62) 24 (7) 2443 (78) 0.44 0.49
Burst 7, ave amplitude µV 31 (9) 2297 (59) 24 (7) 2342 (74) 0.49 0.63
Burst 8, ave amplitude µV 31 (9) 2226 (58) 24 (7) 2255 (72) 0.4 0.77
Burst 9, ave amplitude µV 31 (9) 2164 (59) 24 (7) 2178 (70) 0.39 0.89
Burst 10, ave amplitude µV 31 (9) 2123 (57) 24 (7) 2128 (69) 0.42 0.95
Burst 2-4 ave amplitude vs Burst 1 % 31 (9) 180.1 (3.9) 24 (7) 178.6 (3.1) 0.46 0.79
Burst 8-10 ave amplitude vs Burst 2-4 % 31 (9) 92 (0.9) 24 (7) 88.4 (0.9) 0.25 0.024 *

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 31 (9) 80.8 (4) 24 (7) 77.9 (0.9) 0.19 0.59
LTP based on amplitude % 31 (9) 174.9 (3.9) 24 (7) 166 (3.8) 0.83 0.075

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT9
mutant7

 

Individual Mice

Mouse IDGenotype
wAc0ad8f537WT
wBf4a5b6bfaWT
wB19ccd63f2WT
wB5e90ae86fWT
wA02dd11a6eWT
wB64e62624cWT
wAf1ba97fecWT
wBe3ea0a153WT
wA5e90ae86fWT
mA19ccd63f2mutant
mBf1ba97fecmutant
mA64e62624cmutant
mB02dd11a6emutant
mAf4a5b6bfamutant
mBc0ad8f537mutant
mAe3ea0a153mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAe3ea0a153 mutant 1 58A e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 2 62A e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 3 15B e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 4 55A e3ea0a153_slices_4-5-6-7.zip
mB02dd11a6e mutant 5 52A 02dd11a6e_slices_4-5-6-7.zip
mB02dd11a6e mutant 6 12B 02dd11a6e_slices_4-5-6-7.zip
mB02dd11a6e mutant 7 42B 02dd11a6e_slices_4-5-6-7.zip
mAf4a5b6bfa mutant 1 12A f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 2 62A f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 3 55B f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 4 55A f4a5b6bfa_slices_4-5-6-7.zip
mBc0ad8f537 mutant 5 62A c0ad8f537_slices_4-5-6-7.zip
mBc0ad8f537 mutant 6 12B c0ad8f537_slices_4-5-6-7.zip
mBc0ad8f537 mutant 7 62B c0ad8f537_slices_4-5-6-7.zip
mA19ccd63f2 mutant 1 46A 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 2 62A 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 3 46B 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 4 24A 19ccd63f2_slices_4-5-6-7.zip
mBf1ba97fec mutant 5 71A f1ba97fec_slices_4-5-6-7.zip
mBf1ba97fec mutant 6 46B f1ba97fec_slices_4-5-6-7.zip
mBf1ba97fec mutant 7 73B f1ba97fec_slices_4-5-6-7.zip
mA64e62624c mutant 1 46A 64e62624c_slices_1-2-3-x.zip
mA64e62624c mutant 2 54A 64e62624c_slices_1-2-3-x.zip
mA64e62624c mutant 3 55B 64e62624c_slices_1-2-3-x.zip
wA5e90ae86f WT 1 46A 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 2 52A 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 3 46B 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 4 55A 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 5 62A 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 6 55B 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 7 43B 5e90ae86f_slices_4-5-6-7.zip
wBe3ea0a153 WT 5 62A e3ea0a153_slices_4-5-6-7.zip
wBe3ea0a153 WT 6 38B e3ea0a153_slices_4-5-6-7.zip
wBe3ea0a153 WT 7 62B e3ea0a153_slices_4-5-6-7.zip
wA02dd11a6e WT 2 62A 02dd11a6e_slices_1-2-3-x.zip
wA02dd11a6e WT 3 46B 02dd11a6e_slices_1-2-3-x.zip
wA02dd11a6e WT 4 55A 02dd11a6e_slices_4-5-6-7.zip
wBf4a5b6bfa WT 5 54A f4a5b6bfa_slices_4-5-6-7.zip
wBf4a5b6bfa WT 6 12B f4a5b6bfa_slices_4-5-6-7.zip
wBf4a5b6bfa WT 7 62B f4a5b6bfa_slices_4-5-6-7.zip
wAc0ad8f537 WT 1 46A c0ad8f537_slices_1-2-3-x.zip
wAc0ad8f537 WT 2 61A c0ad8f537_slices_1-2-3-x.zip
wAc0ad8f537 WT 3 46B c0ad8f537_slices_1-2-3-x.zip
wAc0ad8f537 WT 4 55A c0ad8f537_slices_4-5-6-7.zip
wB19ccd63f2 WT 5 62A 19ccd63f2_slices_4-5-6-7.zip
wB19ccd63f2 WT 6 46B 19ccd63f2_slices_4-5-6-7.zip
wB19ccd63f2 WT 7 73B 19ccd63f2_slices_4-5-6-7.zip
wAf1ba97fec WT 1 55A f1ba97fec_slices_1-2-3-x.zip
wAf1ba97fec WT 2 61A f1ba97fec_slices_1-2-3-x.zip
wAf1ba97fec WT 3 46B f1ba97fec_slices_1-2-3-x.zip
wAf1ba97fec WT 4 12A f1ba97fec_slices_4-5-6-7.zip
wB64e62624c WT 4 12A 64e62624c_slices_4-5-6-7.zip
wB64e62624c WT 5 61A 64e62624c_slices_4-5-6-7.zip
wB64e62624c WT 6 46B 64e62624c_slices_4-5-6-7.zip
wB64e62624c WT 7 54B 64e62624c_slices_4-5-6-7.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAe3ea0a153 mutant 1 58A e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 2 62A e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 3 15B e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 4 55A e3ea0a153_slices_4-5-6-7.zip
mB02dd11a6e mutant 5 52A 02dd11a6e_slices_4-5-6-7.zip
mB02dd11a6e mutant 6 12B 02dd11a6e_slices_4-5-6-7.zip
mB02dd11a6e mutant 7 42B 02dd11a6e_slices_4-5-6-7.zip
mAf4a5b6bfa mutant 1 12A f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 2 62A f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 3 55B f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 4 55A f4a5b6bfa_slices_4-5-6-7.zip
mBc0ad8f537 mutant 5 62A c0ad8f537_slices_4-5-6-7.zip
mBc0ad8f537 mutant 6 12B c0ad8f537_slices_4-5-6-7.zip
mBc0ad8f537 mutant 7 62B c0ad8f537_slices_4-5-6-7.zip
mA19ccd63f2 mutant 1 46A 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 2 62A 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 3 46B 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 4 24A 19ccd63f2_slices_4-5-6-7.zip
mBf1ba97fec mutant 5 71A f1ba97fec_slices_4-5-6-7.zip
mBf1ba97fec mutant 6 46B f1ba97fec_slices_4-5-6-7.zip
mBf1ba97fec mutant 7 73B f1ba97fec_slices_4-5-6-7.zip
mA64e62624c mutant 1 46A 64e62624c_slices_1-2-3-x.zip
mA64e62624c mutant 2 54A 64e62624c_slices_1-2-3-x.zip
mA64e62624c mutant 3 55B 64e62624c_slices_1-2-3-x.zip
wA5e90ae86f WT 1 46A 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 2 52A 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 3 46B 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 4 55A 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 5 62A 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 6 55B 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 7 43B 5e90ae86f_slices_4-5-6-7.zip
wBe3ea0a153 WT 5 62A e3ea0a153_slices_4-5-6-7.zip
wBe3ea0a153 WT 6 38B e3ea0a153_slices_4-5-6-7.zip
wBe3ea0a153 WT 7 62B e3ea0a153_slices_4-5-6-7.zip
wA02dd11a6e WT 2 62A 02dd11a6e_slices_1-2-3-x.zip
wA02dd11a6e WT 3 46B 02dd11a6e_slices_1-2-3-x.zip
wA02dd11a6e WT 4 55A 02dd11a6e_slices_4-5-6-7.zip
wBf4a5b6bfa WT 5 54A f4a5b6bfa_slices_4-5-6-7.zip
wBf4a5b6bfa WT 6 12B f4a5b6bfa_slices_4-5-6-7.zip
wBf4a5b6bfa WT 7 62B f4a5b6bfa_slices_4-5-6-7.zip
wAc0ad8f537 WT 1 46A c0ad8f537_slices_1-2-3-x.zip
wAc0ad8f537 WT 2 61A c0ad8f537_slices_1-2-3-x.zip
wAc0ad8f537 WT 3 46B c0ad8f537_slices_1-2-3-x.zip
wAc0ad8f537 WT 4 55A c0ad8f537_slices_4-5-6-7.zip
wB19ccd63f2 WT 5 62A 19ccd63f2_slices_4-5-6-7.zip
wB19ccd63f2 WT 6 46B 19ccd63f2_slices_4-5-6-7.zip
wB19ccd63f2 WT 7 73B 19ccd63f2_slices_4-5-6-7.zip
wAf1ba97fec WT 1 55A f1ba97fec_slices_1-2-3-x.zip
wAf1ba97fec WT 2 61A f1ba97fec_slices_1-2-3-x.zip
wAf1ba97fec WT 3 46B f1ba97fec_slices_1-2-3-x.zip
wAf1ba97fec WT 4 12A f1ba97fec_slices_4-5-6-7.zip
wB64e62624c WT 4 12A 64e62624c_slices_4-5-6-7.zip
wB64e62624c WT 5 61A 64e62624c_slices_4-5-6-7.zip
wB64e62624c WT 6 46B 64e62624c_slices_4-5-6-7.zip
wB64e62624c WT 7 54B 64e62624c_slices_4-5-6-7.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAe3ea0a153 mutant 1 58A e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 2 62A e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 3 15B e3ea0a153_slices_1-2-3-x.zip
mAe3ea0a153 mutant 4 55A e3ea0a153_slices_4-5-6-7.zip
mB02dd11a6e mutant 5 52A 02dd11a6e_slices_4-5-6-7.zip
mB02dd11a6e mutant 6 12B 02dd11a6e_slices_4-5-6-7.zip
mB02dd11a6e mutant 7 42B 02dd11a6e_slices_4-5-6-7.zip
mAf4a5b6bfa mutant 1 12A f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 2 62A f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 3 55B f4a5b6bfa_slices_1-2-3-x.zip
mAf4a5b6bfa mutant 4 55A f4a5b6bfa_slices_4-5-6-7.zip
mBc0ad8f537 mutant 5 62A c0ad8f537_slices_4-5-6-7.zip
mBc0ad8f537 mutant 6 12B c0ad8f537_slices_4-5-6-7.zip
mBc0ad8f537 mutant 7 62B c0ad8f537_slices_4-5-6-7.zip
mA19ccd63f2 mutant 1 46A 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 2 62A 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 3 46B 19ccd63f2_slices_1-2-3-x.zip
mA19ccd63f2 mutant 4 24A 19ccd63f2_slices_4-5-6-7.zip
mBf1ba97fec mutant 5 71A f1ba97fec_slices_4-5-6-7.zip
mBf1ba97fec mutant 6 46B f1ba97fec_slices_4-5-6-7.zip
mBf1ba97fec mutant 7 73B f1ba97fec_slices_4-5-6-7.zip
mA64e62624c mutant 1 46A 64e62624c_slices_1-2-3-x.zip
mA64e62624c mutant 2 54A 64e62624c_slices_1-2-3-x.zip
mA64e62624c mutant 3 55B 64e62624c_slices_1-2-3-x.zip
wA5e90ae86f WT 1 46A 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 2 52A 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 3 46B 5e90ae86f_slices_1-2-3-x.zip
wA5e90ae86f WT 4 55A 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 5 62A 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 6 55B 5e90ae86f_slices_4-5-6-7.zip
wB5e90ae86f WT 7 43B 5e90ae86f_slices_4-5-6-7.zip
wBe3ea0a153 WT 5 62A e3ea0a153_slices_4-5-6-7.zip
wBe3ea0a153 WT 6 38B e3ea0a153_slices_4-5-6-7.zip
wBe3ea0a153 WT 7 62B e3ea0a153_slices_4-5-6-7.zip
wA02dd11a6e WT 2 62A 02dd11a6e_slices_1-2-3-x.zip
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wBf4a5b6bfa WT 5 54A f4a5b6bfa_slices_4-5-6-7.zip
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Data Files

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