G2C::Electrophysiology

Tanc1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices. With heterozygous genotype and no electrophysiological variables significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Tanc1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 24 (7) 2840 (170) 16 (5) 3070 (150) 0.22 0.44
PPF at 50ms, ampl ratio % 24 (7) 169.1 (2.9) 16 (5) 168.9 (4.2) 0.069 0.97
Burst 1, PPF at 10ms, ampl ratio % 24 (7) 155.9 (4.7) 16 (5) 143.3 (6.7) 0.023 * 0.28
Burst 1, EPSP3 depr, ampl ratio % 24 (7) 66.3 (1.7) 16 (5) 66.2 (2.2) 0.2 0.98
Burst 1, ave amplitude µV 24 (7) 1326 (67) 16 (5) 1505 (54) 0.12 0.15
Burst 2, ave amplitude µV 24 (7) 2143 (110) 16 (5) 2420 (89) 0.12 0.17
Burst 3, ave amplitude µV 24 (7) 2328 (119) 16 (5) 2616 (98) 0.22 0.17
Burst 4, ave amplitude µV 24 (7) 2354 (123) 16 (5) 2599 (98) 0.3 0.22
Burst 5, ave amplitude µV 24 (7) 2304 (116) 16 (5) 2536 (92) 0.4 0.2
Burst 6, ave amplitude µV 24 (7) 2241 (115) 16 (5) 2459 (84) 0.37 0.22
Burst 7, ave amplitude µV 24 (7) 2171 (110) 16 (5) 2382 (77) 0.46 0.19
Burst 8, ave amplitude µV 24 (7) 2111 (108) 16 (5) 2323 (74) 0.42 0.19
Burst 9, ave amplitude µV 24 (7) 2055 (107) 16 (5) 2275 (72) 0.43 0.17
Burst 10, ave amplitude µV 24 (7) 2010 (108) 16 (5) 2233 (71) 0.43 0.17
Burst 2-4 ave amplitude vs Burst 1 % 24 (7) 172 (3.2) 16 (5) 171.9 (5.2) 0.24 0.99
Burst 8-10 ave amplitude vs Burst 2-4 % 24 (7) 90.6 (1.1) 16 (5) 90.1 (0.9) 0.25 0.78
LTP vs PTP, amplitude ratio % 24 (7) 81.9 (1.1) 16 (5) 86 (1.5) 0.22 0.075
LTP based on amplitude % 24 (7) 165.7 (3.7) 16 (5) 167.1 (4.3) 0.21 0.85

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 24 (7) 2840 (170) 16 (5) 3070 (150) 0.22 0.44

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 24 (7) 169.1 (2.9) 16 (5) 168.9 (4.2) 0.069 0.97

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 24 (7) 155.9 (4.7) 16 (5) 143.3 (6.7) 0.023 * 0.28
Burst 1, EPSP3 depr, ampl ratio % 24 (7) 66.3 (1.7) 16 (5) 66.2 (2.2) 0.2 0.98
Burst 1, ave amplitude µV 24 (7) 1326 (67) 16 (5) 1505 (54) 0.12 0.15
Burst 2, ave amplitude µV 24 (7) 2143 (110) 16 (5) 2420 (89) 0.12 0.17
Burst 3, ave amplitude µV 24 (7) 2328 (119) 16 (5) 2616 (98) 0.22 0.17
Burst 4, ave amplitude µV 24 (7) 2354 (123) 16 (5) 2599 (98) 0.3 0.22
Burst 5, ave amplitude µV 24 (7) 2304 (116) 16 (5) 2536 (92) 0.4 0.2
Burst 6, ave amplitude µV 24 (7) 2241 (115) 16 (5) 2459 (84) 0.37 0.22
Burst 7, ave amplitude µV 24 (7) 2171 (110) 16 (5) 2382 (77) 0.46 0.19
Burst 8, ave amplitude µV 24 (7) 2111 (108) 16 (5) 2323 (74) 0.42 0.19
Burst 9, ave amplitude µV 24 (7) 2055 (107) 16 (5) 2275 (72) 0.43 0.17
Burst 10, ave amplitude µV 24 (7) 2010 (108) 16 (5) 2233 (71) 0.43 0.17
Burst 2-4 ave amplitude vs Burst 1 % 24 (7) 172 (3.2) 16 (5) 171.9 (5.2) 0.24 0.99
Burst 8-10 ave amplitude vs Burst 2-4 % 24 (7) 90.6 (1.1) 16 (5) 90.1 (0.9) 0.25 0.78

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 24 (7) 81.9 (1.1) 16 (5) 86 (1.5) 0.22 0.075
LTP based on amplitude % 24 (7) 165.7 (3.7) 16 (5) 167.1 (4.3) 0.21 0.85

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT7
mutant5

 

Individual Mice

Mouse IDGenotype
wB7cc09b33fWT
wA4652feae2WT
wBd1af10c9eWT
wA4ef9dab87WT
wB5638f2a5bWT
wB4652feae2WT
wA44d47a97cWT
mA7cc09b33fmutant
mB4ef9dab87mutant
mA5638f2a5bmutant
mAd1af10c9emutant
mB44d47a97cmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAd1af10c9e mutant 2 61B d1af10c9e_slices_1-2-3-4.zip
mAd1af10c9e mutant 3 46C d1af10c9e_slices_1-2-3-4.zip
mAd1af10c9e mutant 4 53D d1af10c9e_slices_1-2-3-4.zip
mB4ef9dab87 mutant 5 12A 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 6 61A 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 7 58B 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 8 62B 4ef9dab87_slices_5-6-7-8.zip
mA7cc09b33f mutant 2 61B 7cc09b33f_slices_1-2-3-4.zip
mA7cc09b33f mutant 3 42C 7cc09b33f_slices_1-2-3-4.zip
mA7cc09b33f mutant 4 52D 7cc09b33f_slices_1-2-3-4.zip
mB44d47a97c mutant 7 38B 44d47a97c_slices_5-6-7-8.zip
mB44d47a97c mutant 8 52B 44d47a97c_slices_5-6-7-8.zip
mA5638f2a5b mutant 1 24A 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 2 15B 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 3 12C 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 4 38D 5638f2a5b_slices_1-2-3-4.zip
wBd1af10c9e WT 5 46A d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 6 52A d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 7 55B d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 8 42B d1af10c9e_slices_5-6-7-8.zip
wA4ef9dab87 WT 1 62A 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 2 46B 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 3 53C 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 4 61D 4ef9dab87_slices_1-2-3-4.zip
wB7cc09b33f WT 5 46A 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 6 41A 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 7 12B 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 8 62B 7cc09b33f_slices_5-6-7-8.zip
wA44d47a97c WT 1 44A 44d47a97c_slices_1-2-3-4.zip
wA44d47a97c WT 2 53B 44d47a97c_slices_1-2-3-4.zip
wA44d47a97c WT 4 53D 44d47a97c_slices_1-2-3-4.zip
wB4652feae2 WT 1 44A 4652feae2_slices_1-2-3-4.zip
wA4652feae2 WT 3 46C 4652feae2_slices_1-2-3-4.zip
wB4652feae2 WT 4 52D 4652feae2_slices_1-2-3-4.zip
wB4652feae2 WT 5 46A 4652feae2_slices_5-6-7-x.zip
wB4652feae2 WT 6 54A 4652feae2_slices_5-6-7-x.zip
wB5638f2a5b WT 5 12A 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 6 54A 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 7 46B 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 8 43B 5638f2a5b_slices_5-6-7-8.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAd1af10c9e mutant 2 61B d1af10c9e_slices_1-2-3-4.zip
mAd1af10c9e mutant 3 46C d1af10c9e_slices_1-2-3-4.zip
mAd1af10c9e mutant 4 53D d1af10c9e_slices_1-2-3-4.zip
mB4ef9dab87 mutant 5 12A 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 6 61A 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 7 58B 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 8 62B 4ef9dab87_slices_5-6-7-8.zip
mA7cc09b33f mutant 2 61B 7cc09b33f_slices_1-2-3-4.zip
mA7cc09b33f mutant 3 42C 7cc09b33f_slices_1-2-3-4.zip
mA7cc09b33f mutant 4 52D 7cc09b33f_slices_1-2-3-4.zip
mB44d47a97c mutant 7 38B 44d47a97c_slices_5-6-7-8.zip
mB44d47a97c mutant 8 52B 44d47a97c_slices_5-6-7-8.zip
mA5638f2a5b mutant 1 24A 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 2 15B 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 3 12C 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 4 38D 5638f2a5b_slices_1-2-3-4.zip
wBd1af10c9e WT 5 46A d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 6 52A d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 7 55B d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 8 42B d1af10c9e_slices_5-6-7-8.zip
wA4ef9dab87 WT 1 62A 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 2 46B 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 3 53C 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 4 61D 4ef9dab87_slices_1-2-3-4.zip
wB7cc09b33f WT 5 46A 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 6 41A 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 7 12B 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 8 62B 7cc09b33f_slices_5-6-7-8.zip
wA44d47a97c WT 1 44A 44d47a97c_slices_1-2-3-4.zip
wA44d47a97c WT 2 53B 44d47a97c_slices_1-2-3-4.zip
wA44d47a97c WT 4 53D 44d47a97c_slices_1-2-3-4.zip
wB4652feae2 WT 1 44A 4652feae2_slices_1-2-3-4.zip
wA4652feae2 WT 3 46C 4652feae2_slices_1-2-3-4.zip
wB4652feae2 WT 4 52D 4652feae2_slices_1-2-3-4.zip
wB4652feae2 WT 5 46A 4652feae2_slices_5-6-7-x.zip
wB4652feae2 WT 6 54A 4652feae2_slices_5-6-7-x.zip
wB5638f2a5b WT 5 12A 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 6 54A 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 7 46B 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 8 43B 5638f2a5b_slices_5-6-7-8.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAd1af10c9e mutant 2 61B d1af10c9e_slices_1-2-3-4.zip
mAd1af10c9e mutant 3 46C d1af10c9e_slices_1-2-3-4.zip
mAd1af10c9e mutant 4 53D d1af10c9e_slices_1-2-3-4.zip
mB4ef9dab87 mutant 5 12A 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 6 61A 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 7 58B 4ef9dab87_slices_5-6-7-8.zip
mB4ef9dab87 mutant 8 62B 4ef9dab87_slices_5-6-7-8.zip
mA7cc09b33f mutant 2 61B 7cc09b33f_slices_1-2-3-4.zip
mA7cc09b33f mutant 3 42C 7cc09b33f_slices_1-2-3-4.zip
mA7cc09b33f mutant 4 52D 7cc09b33f_slices_1-2-3-4.zip
mB44d47a97c mutant 7 38B 44d47a97c_slices_5-6-7-8.zip
mB44d47a97c mutant 8 52B 44d47a97c_slices_5-6-7-8.zip
mA5638f2a5b mutant 1 24A 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 2 15B 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 3 12C 5638f2a5b_slices_1-2-3-4.zip
mA5638f2a5b mutant 4 38D 5638f2a5b_slices_1-2-3-4.zip
wBd1af10c9e WT 5 46A d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 6 52A d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 7 55B d1af10c9e_slices_5-6-7-8.zip
wBd1af10c9e WT 8 42B d1af10c9e_slices_5-6-7-8.zip
wA4ef9dab87 WT 1 62A 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 2 46B 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 3 53C 4ef9dab87_slices_1-2-3-4.zip
wA4ef9dab87 WT 4 61D 4ef9dab87_slices_1-2-3-4.zip
wB7cc09b33f WT 5 46A 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 6 41A 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 7 12B 7cc09b33f_slices_5-6-7-8.zip
wB7cc09b33f WT 8 62B 7cc09b33f_slices_5-6-7-8.zip
wA44d47a97c WT 1 44A 44d47a97c_slices_1-2-3-4.zip
wA44d47a97c WT 2 53B 44d47a97c_slices_1-2-3-4.zip
wA44d47a97c WT 4 53D 44d47a97c_slices_1-2-3-4.zip
wB4652feae2 WT 1 44A 4652feae2_slices_1-2-3-4.zip
wA4652feae2 WT 3 46C 4652feae2_slices_1-2-3-4.zip
wB4652feae2 WT 4 52D 4652feae2_slices_1-2-3-4.zip
wB4652feae2 WT 5 46A 4652feae2_slices_5-6-7-x.zip
wB4652feae2 WT 6 54A 4652feae2_slices_5-6-7-x.zip
wB5638f2a5b WT 5 12A 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 6 54A 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 7 46B 5638f2a5b_slices_5-6-7-8.zip
wB5638f2a5b WT 8 43B 5638f2a5b_slices_5-6-7-8.zip

Data Files

File NameSizeChecksum (SHA256)
5638f2a5b_slices_1-2-3-4.zip1484270464dc2201a63f6c14b258c06545108570d73666862dd9e5085a1cad234e37156ab
5638f2a5b_slices_5-6-7-8.zip4145697235fb92ef728e9e64b4b2c3ec35670c9986124f3f24e4793784f5bf8aefdc210e
7cc09b33f_slices_1-2-3-4.zip127992037a0b1b7a1230fcaea45d22dbd2526b2349d0d6c3c5fed6b53f065744343d9900a
d1af10c9e_slices_5-6-7-8.zip416477372ba48dca2b480864806355f6e59a1b3b360d8263d1e6c0f814047e47edf86e16
44d47a97c_slices_5-6-7-8.zip4898546574ca9a2a7371396f83df1b5646593767887fcc1ecb3243219b67e2b2e5b51073
4ef9dab87_slices_1-2-3-4.zip145617913a1d1059b20659e01dbef19ff65f7dedc5ff18a29f1a0eefad44a23cfc797c94a
4652feae2_slices_1-2-3-4.zip140189305cacd9433a7904c10bc773f8f6de11f86ee556b79b7b9d48b1aa198e67c3e0b35
44d47a97c_slices_1-2-3-4.zip1309328708a1743c696c40d154d5de181988f843cbb8c6c6be0b310109fb256b18eb36e5a
4ef9dab87_slices_5-6-7-8.zip44537442ef63f921e1c6115102c071032c676b720120f69e7d7614209918c564cc48909d
d1af10c9e_slices_1-2-3-4.zip129687800beae412c01541c1f96ded56db4bca27428cc97526e11a6dec3e454c51d3acdc5
4652feae2_slices_5-6-7-x.zip3804148968a07c78c50a55ae0743c502a140e3df9b2f686ded2b0412e4c43d686013d9ab
7cc09b33f_slices_5-6-7-8.zip417110472a49cac47176ba98ed9fe4f74e2b20ede18157ddb205ed23182cc3cf579ffb55
© G2C 2014. The Genes to Cognition Programme received funding from The Wellcome Trust and the EU FP7 Framework Programmes:
EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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