G2C::Electrophysiology

CYFIP1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices, with two of nine variables significantly impacted in these mutant slices. In the theta burst stimulation experiment, two electrophysiological variables of five were significantly impacted in mutants. Basal synaptic transmission, paired pulse facilitation and long term potentiation experiments were unaffected. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the CYFIP1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3160 (150) 18 (5) 3160 (150) 0.4 0.98
PPF at 50ms, ampl ratio % 17 (5) 171.1 (3.8) 18 (5) 175.1 (4.9) 0.49 0.54
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 197.8 (6.4) 17 (5) 181.1 (7.2) 0.99 0.0086 **
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 57.2 (1.8) 17 (5) 66.3 (2.6) 0.95 0.0023 **
Burst 1, ave amplitude µV 17 (5) 1200 (38) 17 (5) 1301 (42) 0.53 0.1
Burst 2, ave amplitude µV 17 (5) 1831 (82) 17 (5) 2091 (103) 0.022 * 0.2
Burst 3, ave amplitude µV 17 (5) 2088 (95) 17 (5) 2326 (126) 0.034 * 0.3
Burst 4, ave amplitude µV 17 (5) 2170 (94) 17 (5) 2469 (136) 0.023 * 0.24
Burst 5, ave amplitude µV 17 (5) 2157 (86) 17 (5) 2468 (134) 0.017 * 0.21
Burst 6, ave amplitude µV 17 (5) 2096 (81) 17 (5) 2388 (124) 0.015 * 0.21
Burst 7, ave amplitude µV 17 (5) 2031 (79) 17 (5) 2318 (118) 0.015 * 0.2
Burst 8, ave amplitude µV 17 (5) 1957 (75) 17 (5) 2239 (114) 0.015 * 0.19
Burst 9, ave amplitude µV 17 (5) 1897 (73) 17 (5) 2169 (107) 0.017 * 0.18
Burst 10, ave amplitude µV 17 (5) 1854 (73) 17 (5) 2118 (103) 0.017 * 0.18
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 168.6 (4.4) 17 (5) 175.8 (6.7) 0.0095 ** 0.56
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 94.1 (1.5) 17 (5) 95 (1) 0.3 0.65
LTP vs PTP, amplitude ratio % 17 (5) 78.6 (1) 17 (5) 76.4 (1.1) 0.28 0.19
LTP based on amplitude % 17 (5) 178.8 (5.5) 17 (5) 172.8 (6.9) 0.76 0.43

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3160 (150) 18 (5) 3160 (150) 0.4 0.98

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 17 (5) 171.1 (3.8) 18 (5) 175.1 (4.9) 0.49 0.54

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 197.8 (6.4) 17 (5) 181.1 (7.2) 0.99 0.0086 **
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 57.2 (1.8) 17 (5) 66.3 (2.6) 0.95 0.0023 **
Burst 1, ave amplitude µV 17 (5) 1200 (38) 17 (5) 1301 (42) 0.53 0.1
Burst 2, ave amplitude µV 17 (5) 1831 (82) 17 (5) 2091 (103) 0.022 * 0.2
Burst 3, ave amplitude µV 17 (5) 2088 (95) 17 (5) 2326 (126) 0.034 * 0.3
Burst 4, ave amplitude µV 17 (5) 2170 (94) 17 (5) 2469 (136) 0.023 * 0.24
Burst 5, ave amplitude µV 17 (5) 2157 (86) 17 (5) 2468 (134) 0.017 * 0.21
Burst 6, ave amplitude µV 17 (5) 2096 (81) 17 (5) 2388 (124) 0.015 * 0.21
Burst 7, ave amplitude µV 17 (5) 2031 (79) 17 (5) 2318 (118) 0.015 * 0.2
Burst 8, ave amplitude µV 17 (5) 1957 (75) 17 (5) 2239 (114) 0.015 * 0.19
Burst 9, ave amplitude µV 17 (5) 1897 (73) 17 (5) 2169 (107) 0.017 * 0.18
Burst 10, ave amplitude µV 17 (5) 1854 (73) 17 (5) 2118 (103) 0.017 * 0.18
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 168.6 (4.4) 17 (5) 175.8 (6.7) 0.0095 ** 0.56
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 94.1 (1.5) 17 (5) 95 (1) 0.3 0.65

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 17 (5) 78.6 (1) 17 (5) 76.4 (1.1) 0.28 0.19
LTP based on amplitude % 17 (5) 178.8 (5.5) 17 (5) 172.8 (6.9) 0.76 0.43

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wAd98cc6dc0WT
wB58c639fe5WT
wA8180f7cbaWT
wB94b2ccb8eWT
wB8180f7cbaWT
mA58c639fe5mutant
mBd98cc6dc0mutant
mA94b2ccb8emutant
mB564f570f0mutant
mA564f570f0mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA564f570f0 mutant 1 55A 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 2 54A 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 3 46B 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 4 12A 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 5 52A 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 6 46B 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 7 54B 564f570f0_slices_4-5-6-7.zip
mA58c639fe5 mutant 1 12A 58c639fe5_slices_1-2-3-x.zip
mA58c639fe5 mutant 2 62A 58c639fe5_slices_1-2-3-x.zip
mA58c639fe5 mutant 3 58B 58c639fe5_slices_1-2-3-x.zip
mBd98cc6dc0 mutant 4 12A d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 5 52A d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 6 12B d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 7 52B d98cc6dc0_slices_4-5-6-7.zip
mA94b2ccb8e mutant 1 12A 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 2 54A 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 3 12B 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 4 46A 94b2ccb8e_slices_4-5-6-7.zip
wA8180f7cba WT 1 46A 8180f7cba_slices_1-2-3-x.zip
wA8180f7cba WT 2 61A 8180f7cba_slices_1-2-3-x.zip
wA8180f7cba WT 3 46B 8180f7cba_slices_1-2-3-x.zip
wB8180f7cba WT 4 12A 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 5 61A 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 6 12B 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 7 62B 8180f7cba_slices_4-5-6-7.zip
wB58c639fe5 WT 4 55A 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 5 52A 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 6 46B 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 7 61B 58c639fe5_slices_4-5-6-7.zip
wAd98cc6dc0 WT 1 46A d98cc6dc0_slices_1-2-3-x.zip
wAd98cc6dc0 WT 2 62A d98cc6dc0_slices_1-2-3-x.zip
wAd98cc6dc0 WT 3 46B d98cc6dc0_slices_1-2-3-x.zip
wB94b2ccb8e WT 5 52A 94b2ccb8e_slices_4-5-6-7.zip
wB94b2ccb8e WT 6 46B 94b2ccb8e_slices_4-5-6-7.zip
wB94b2ccb8e WT 7 41B 94b2ccb8e_slices_4-5-6-7.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA564f570f0 mutant 1 55A 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 2 54A 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 3 46B 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 4 12A 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 5 52A 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 6 46B 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 7 54B 564f570f0_slices_4-5-6-7.zip
mA58c639fe5 mutant 1 12A 58c639fe5_slices_1-2-3-x.zip
mA58c639fe5 mutant 2 62A 58c639fe5_slices_1-2-3-x.zip
mA58c639fe5 mutant 3 58B 58c639fe5_slices_1-2-3-x.zip
mBd98cc6dc0 mutant 4 12A d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 5 52A d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 6 12B d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 7 52B d98cc6dc0_slices_4-5-6-7.zip
mA94b2ccb8e mutant 1 12A 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 2 54A 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 3 12B 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 4 46A 94b2ccb8e_slices_4-5-6-7.zip
wA8180f7cba WT 1 46A 8180f7cba_slices_1-2-3-x.zip
wA8180f7cba WT 2 61A 8180f7cba_slices_1-2-3-x.zip
wA8180f7cba WT 3 46B 8180f7cba_slices_1-2-3-x.zip
wB8180f7cba WT 4 12A 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 5 61A 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 6 12B 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 7 62B 8180f7cba_slices_4-5-6-7.zip
wB58c639fe5 WT 4 55A 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 5 52A 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 6 46B 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 7 61B 58c639fe5_slices_4-5-6-7.zip
wAd98cc6dc0 WT 1 46A d98cc6dc0_slices_1-2-3-x.zip
wAd98cc6dc0 WT 2 62A d98cc6dc0_slices_1-2-3-x.zip
wAd98cc6dc0 WT 3 46B d98cc6dc0_slices_1-2-3-x.zip
wB94b2ccb8e WT 5 52A 94b2ccb8e_slices_4-5-6-7.zip
wB94b2ccb8e WT 6 46B 94b2ccb8e_slices_4-5-6-7.zip
wB94b2ccb8e WT 7 41B 94b2ccb8e_slices_4-5-6-7.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA564f570f0 mutant 1 55A 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 2 54A 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 3 46B 564f570f0_slices_1-2-3-x.zip
mA564f570f0 mutant 4 12A 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 5 52A 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 6 46B 564f570f0_slices_4-5-6-7.zip
mB564f570f0 mutant 7 54B 564f570f0_slices_4-5-6-7.zip
mA58c639fe5 mutant 1 12A 58c639fe5_slices_1-2-3-x.zip
mA58c639fe5 mutant 2 62A 58c639fe5_slices_1-2-3-x.zip
mA58c639fe5 mutant 3 58B 58c639fe5_slices_1-2-3-x.zip
mBd98cc6dc0 mutant 4 12A d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 5 52A d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 6 12B d98cc6dc0_slices_4-5-6-7.zip
mBd98cc6dc0 mutant 7 52B d98cc6dc0_slices_4-5-6-7.zip
mA94b2ccb8e mutant 1 12A 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 2 54A 94b2ccb8e_slices_1-2-3-x.zip
mA94b2ccb8e mutant 3 12B 94b2ccb8e_slices_1-2-3-x.zip
wA8180f7cba WT 1 46A 8180f7cba_slices_1-2-3-x.zip
wA8180f7cba WT 2 61A 8180f7cba_slices_1-2-3-x.zip
wA8180f7cba WT 3 46B 8180f7cba_slices_1-2-3-x.zip
wB8180f7cba WT 4 12A 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 5 61A 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 6 12B 8180f7cba_slices_4-5-6-7.zip
wB8180f7cba WT 7 62B 8180f7cba_slices_4-5-6-7.zip
wB58c639fe5 WT 4 55A 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 5 52A 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 6 46B 58c639fe5_slices_4-5-6-7.zip
wB58c639fe5 WT 7 61B 58c639fe5_slices_4-5-6-7.zip
wAd98cc6dc0 WT 1 46A d98cc6dc0_slices_1-2-3-x.zip
wAd98cc6dc0 WT 2 62A d98cc6dc0_slices_1-2-3-x.zip
wAd98cc6dc0 WT 3 46B d98cc6dc0_slices_1-2-3-x.zip
wB94b2ccb8e WT 5 52A 94b2ccb8e_slices_4-5-6-7.zip
wB94b2ccb8e WT 6 46B 94b2ccb8e_slices_4-5-6-7.zip
wB94b2ccb8e WT 7 41B 94b2ccb8e_slices_4-5-6-7.zip

Data Files

File NameSizeChecksum (SHA256)
8180f7cba_slices_1-2-3-x.zip33129538163f81eb6a7a7cc18330c668ddc2ac0bfdb4a321de9b45dc6efe683504ce3add
8180f7cba_slices_4-5-6-7.zip49517727c18940362cd75e3ec809dc6f3483366e15773dc937b62367d29f9171d716ed09
d98cc6dc0_slices_4-5-6-7.zip5361126783b09599497c659f92de285645d2a0137a9a4ebde97b6d21a60cbfc6abe57b8b
58c639fe5_slices_1-2-3-x.zip314581950215ac236c23e4e4a12e4e48a8d71d5d39495b6dd8d05803ca6c9048a120e52c
564f570f0_slices_1-2-3-x.zip328259162785369fc385d8728ecead8af6c71e2e6bca13da878be20afdecf6c1d6109167
564f570f0_slices_4-5-6-7.zip46267596138d45b2260a0336da015155386f55903654f2c3c3f1a1d7dc82946a73b0fed9
d98cc6dc0_slices_1-2-3-x.zip38012051bb5ac7b1d20a39480193b5e157fb60f3e3807e399686eaa1e5ddadeeb852379b
94b2ccb8e_slices_1-2-3-x.zip32312399b5b3be1c23deff82d70c4cda55b32984fed0d05edf89b16c7945d3c7b93fcf47
58c639fe5_slices_4-5-6-7.zip4806998552f5cba719670fe6749901fd8809bbfda34b36b0622bd7ac7909c8a0c3ab2ba6
94b2ccb8e_slices_4-5-6-7.zip5216181850239dafa0adfc8a735d835f25ef6b88cc444a0a718d774a76e7460911f88749
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