G2C::Electrophysiology

CINAP knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with only one variable, burst 8-10 ave amplitude vs burst 2-4, significantly increased by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the CINAP knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 27 (8) 3140 (140) 23 (8) 3250 (140) 0.76 0.54
PPF at 50ms, ampl ratio % 27 (8) 179.5 (3.6) 23 (8) 177 (4.2) 0.37 0.68
Burst 1, PPF at 10ms, ampl ratio % 27 (8) 189.6 (4.8) 22 (8) 193.7 (6.4) 0.33 0.63
Burst 1, EPSP3 depr, ampl ratio % 27 (8) 62.8 (1.6) 22 (8) 59.8 (2.1) 0.81 0.19
Burst 1, ave amplitude µV 27 (8) 1404 (61) 22 (8) 1386 (69) 0.92 0.8
Burst 2, ave amplitude µV 27 (8) 2239 (105) 22 (8) 2024 (85) 0.45 0.15
Burst 3, ave amplitude µV 27 (8) 2520 (120) 22 (8) 2310 (100) 0.56 0.22
Burst 4, ave amplitude µV 27 (8) 2590 (120) 22 (8) 2420 (100) 0.63 0.29
Burst 5, ave amplitude µV 26 (8) 2560 (110) 22 (8) 2393 (96) 0.63 0.25
Burst 6, ave amplitude µV 26 (8) 2500 (103) 22 (8) 2334 (93) 0.69 0.22
Burst 7, ave amplitude µV 26 (8) 2410 (95) 22 (8) 2280 (88) 0.66 0.3
Burst 8, ave amplitude µV 26 (8) 2341 (92) 22 (8) 2209 (88) 0.62 0.29
Burst 9, ave amplitude µV 26 (8) 2271 (89) 22 (8) 2162 (90) 0.6 0.38
Burst 10, ave amplitude µV 26 (8) 2223 (88) 22 (8) 2115 (90) 0.49 0.41
Burst 2-4 ave amplitude vs Burst 1 % 27 (8) 174.5 (4.4) 22 (8) 164 (4.4) 0.4 0.13
Burst 8-10 ave amplitude vs Burst 2-4 % 26 (8) 93 (1.3) 22 (8) 97 (0.8) 0.48 0.028 *
LTP vs PTP, amplitude ratio % 27 (8) 81.3 (2.1) 22 (8) 90.3 (9.3) 0.51 0.31
LTP based on amplitude % 27 (8) 171.9 (3.9) 22 (8) 183.1 (5.4) 0.2 0.15

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 27 (8) 3140 (140) 23 (8) 3250 (140) 0.76 0.54

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 27 (8) 179.5 (3.6) 23 (8) 177 (4.2) 0.37 0.68

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 27 (8) 189.6 (4.8) 22 (8) 193.7 (6.4) 0.33 0.63
Burst 1, EPSP3 depr, ampl ratio % 27 (8) 62.8 (1.6) 22 (8) 59.8 (2.1) 0.81 0.19
Burst 1, ave amplitude µV 27 (8) 1404 (61) 22 (8) 1386 (69) 0.92 0.8
Burst 2, ave amplitude µV 27 (8) 2239 (105) 22 (8) 2024 (85) 0.45 0.15
Burst 3, ave amplitude µV 27 (8) 2520 (120) 22 (8) 2310 (100) 0.56 0.22
Burst 4, ave amplitude µV 27 (8) 2590 (120) 22 (8) 2420 (100) 0.63 0.29
Burst 5, ave amplitude µV 26 (8) 2560 (110) 22 (8) 2393 (96) 0.63 0.25
Burst 6, ave amplitude µV 26 (8) 2500 (103) 22 (8) 2334 (93) 0.69 0.22
Burst 7, ave amplitude µV 26 (8) 2410 (95) 22 (8) 2280 (88) 0.66 0.3
Burst 8, ave amplitude µV 26 (8) 2341 (92) 22 (8) 2209 (88) 0.62 0.29
Burst 9, ave amplitude µV 26 (8) 2271 (89) 22 (8) 2162 (90) 0.6 0.38
Burst 10, ave amplitude µV 26 (8) 2223 (88) 22 (8) 2115 (90) 0.49 0.41
Burst 2-4 ave amplitude vs Burst 1 % 27 (8) 174.5 (4.4) 22 (8) 164 (4.4) 0.4 0.13
Burst 8-10 ave amplitude vs Burst 2-4 % 26 (8) 93 (1.3) 22 (8) 97 (0.8) 0.48 0.028 *

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 27 (8) 81.3 (2.1) 22 (8) 90.3 (9.3) 0.51 0.31
LTP based on amplitude % 27 (8) 171.9 (3.9) 22 (8) 183.1 (5.4) 0.2 0.15

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT8
mutant8

 

Individual Mice

Mouse IDGenotype
wBf715d5511WT
wAa1d905a11WT
wB9023e5745WT
wA3de8f2e17WT
wAe40eff646WT
wB37e0450acWT
wBcdc2f1817WT
wBe40eff646WT
mAcdc2f1817mutant
mB997de7e37mutant
mA37e0450acmutant
mAf715d5511mutant
mBa1d905a11mutant
mA997de7e37mutant
mA9023e5745mutant
mB3de8f2e17mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA9023e5745 mutant 1 55A 9023e5745_slices_1-2-3-x.zip
mA9023e5745 mutant 3 55B 9023e5745_slices_1-2-3-x.zip
mA9023e5745 mutant 4 15A 9023e5745_slices_4-5-6-7.zip
mBa1d905a11 mutant 5 43A a1d905a11_slices_4-5-6-7.zip
mBa1d905a11 mutant 6 46B a1d905a11_slices_4-5-6-7.zip
mBa1d905a11 mutant 7 43B a1d905a11_slices_4-5-6-7.zip
mA37e0450ac mutant 1 12A 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 2 52A 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 3 55B 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 4 58A 37e0450ac_slices_4-5-6-7.zip
mAf715d5511 mutant 2 52A f715d5511_slices_1-2-3-x.zip
mAf715d5511 mutant 3 12B f715d5511_slices_1-2-3-x.zip
mB3de8f2e17 mutant 5 62A 3de8f2e17_slices_4-5-6-7.zip
mB3de8f2e17 mutant 6 55B 3de8f2e17_slices_4-5-6-7.zip
mB3de8f2e17 mutant 7 61B 3de8f2e17_slices_4-5-6-7.zip
mA997de7e37 mutant 1 46A 997de7e37_slices_1-2-3-x.zip
mA997de7e37 mutant 2 42A 997de7e37_slices_1-2-3-x.zip
mA997de7e37 mutant 3 15B 997de7e37_slices_1-2-3-x.zip
mB997de7e37 mutant 4 55A 997de7e37_slices_4-5-6-x.zip
mB997de7e37 mutant 5 54A 997de7e37_slices_4-5-6-x.zip
mB997de7e37 mutant 6 46B 997de7e37_slices_4-5-6-x.zip
mAcdc2f1817 mutant 1 46A cdc2f1817_slices_1-2-3-x.zip
mAcdc2f1817 mutant 2 52A cdc2f1817_slices_1-2-3-x.zip
mAcdc2f1817 mutant 3 15B cdc2f1817_slices_1-2-3-x.zip
wB9023e5745 WT 2 52A 9023e5745_slices_1-2-3-x.zip
wB9023e5745 WT 5 52A 9023e5745_slices_4-5-6-7.zip
wB9023e5745 WT 6 15B 9023e5745_slices_4-5-6-7.zip
wB9023e5745 WT 7 54B 9023e5745_slices_4-5-6-7.zip
wAa1d905a11 WT 1 12A a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 2 52A a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 3 15B a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 4 38A a1d905a11_slices_4-5-6-7.zip
wB37e0450ac WT 5 61A 37e0450ac_slices_4-5-6-7.zip
wB37e0450ac WT 6 46B 37e0450ac_slices_4-5-6-7.zip
wB37e0450ac WT 7 52B 37e0450ac_slices_4-5-6-7.zip
wBf715d5511 WT 5 42A f715d5511_slices_4-5-6-7.zip
wBf715d5511 WT 7 52B f715d5511_slices_4-5-6-7.zip
wA3de8f2e17 WT 2 61A 3de8f2e17_slices_1-2-3-x.zip
wA3de8f2e17 WT 3 55B 3de8f2e17_slices_1-2-3-x.zip
wA3de8f2e17 WT 4 12A 3de8f2e17_slices_4-5-6-7.zip
wBcdc2f1817 WT 4 58A cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 5 61A cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 6 46B cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 7 43B cdc2f1817_slices_4-5-6-7.zip
wAe40eff646 WT 1 12A e40eff646_slices_1-2-3-x.zip
wAe40eff646 WT 2 41A e40eff646_slices_1-2-3-x.zip
wAe40eff646 WT 3 12B e40eff646_slices_1-2-3-x.zip
wBe40eff646 WT 4 38A e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 5 54A e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 6 55B e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 7 62B e40eff646_slices_4-5-6-7.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA9023e5745 mutant 1 55A 9023e5745_slices_1-2-3-x.zip
mA9023e5745 mutant 3 55B 9023e5745_slices_1-2-3-x.zip
mA9023e5745 mutant 4 15A 9023e5745_slices_4-5-6-7.zip
mBa1d905a11 mutant 5 43A a1d905a11_slices_4-5-6-7.zip
mBa1d905a11 mutant 6 46B a1d905a11_slices_4-5-6-7.zip
mBa1d905a11 mutant 7 43B a1d905a11_slices_4-5-6-7.zip
mA37e0450ac mutant 1 12A 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 2 52A 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 3 55B 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 4 58A 37e0450ac_slices_4-5-6-7.zip
mAf715d5511 mutant 2 52A f715d5511_slices_1-2-3-x.zip
mAf715d5511 mutant 3 12B f715d5511_slices_1-2-3-x.zip
mB3de8f2e17 mutant 5 62A 3de8f2e17_slices_4-5-6-7.zip
mB3de8f2e17 mutant 6 55B 3de8f2e17_slices_4-5-6-7.zip
mB3de8f2e17 mutant 7 61B 3de8f2e17_slices_4-5-6-7.zip
mA997de7e37 mutant 1 46A 997de7e37_slices_1-2-3-x.zip
mA997de7e37 mutant 2 42A 997de7e37_slices_1-2-3-x.zip
mA997de7e37 mutant 3 15B 997de7e37_slices_1-2-3-x.zip
mB997de7e37 mutant 4 55A 997de7e37_slices_4-5-6-x.zip
mB997de7e37 mutant 5 54A 997de7e37_slices_4-5-6-x.zip
mB997de7e37 mutant 6 46B 997de7e37_slices_4-5-6-x.zip
mAcdc2f1817 mutant 1 46A cdc2f1817_slices_1-2-3-x.zip
mAcdc2f1817 mutant 2 52A cdc2f1817_slices_1-2-3-x.zip
mAcdc2f1817 mutant 3 15B cdc2f1817_slices_1-2-3-x.zip
wB9023e5745 WT 2 52A 9023e5745_slices_1-2-3-x.zip
wB9023e5745 WT 5 52A 9023e5745_slices_4-5-6-7.zip
wB9023e5745 WT 6 15B 9023e5745_slices_4-5-6-7.zip
wB9023e5745 WT 7 54B 9023e5745_slices_4-5-6-7.zip
wAa1d905a11 WT 1 12A a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 2 52A a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 3 15B a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 4 38A a1d905a11_slices_4-5-6-7.zip
wB37e0450ac WT 5 61A 37e0450ac_slices_4-5-6-7.zip
wB37e0450ac WT 6 46B 37e0450ac_slices_4-5-6-7.zip
wB37e0450ac WT 7 52B 37e0450ac_slices_4-5-6-7.zip
wBf715d5511 WT 5 42A f715d5511_slices_4-5-6-7.zip
wBf715d5511 WT 7 52B f715d5511_slices_4-5-6-7.zip
wA3de8f2e17 WT 2 61A 3de8f2e17_slices_1-2-3-x.zip
wA3de8f2e17 WT 3 55B 3de8f2e17_slices_1-2-3-x.zip
wA3de8f2e17 WT 4 12A 3de8f2e17_slices_4-5-6-7.zip
wBcdc2f1817 WT 4 58A cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 5 61A cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 6 46B cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 7 43B cdc2f1817_slices_4-5-6-7.zip
wAe40eff646 WT 1 12A e40eff646_slices_1-2-3-x.zip
wAe40eff646 WT 2 41A e40eff646_slices_1-2-3-x.zip
wAe40eff646 WT 3 12B e40eff646_slices_1-2-3-x.zip
wBe40eff646 WT 4 38A e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 5 54A e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 6 55B e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 7 62B e40eff646_slices_4-5-6-7.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA9023e5745 mutant 1 55A 9023e5745_slices_1-2-3-x.zip
mA9023e5745 mutant 3 55B 9023e5745_slices_1-2-3-x.zip
mA9023e5745 mutant 4 15A 9023e5745_slices_4-5-6-7.zip
mBa1d905a11 mutant 5 43A a1d905a11_slices_4-5-6-7.zip
mBa1d905a11 mutant 6 46B a1d905a11_slices_4-5-6-7.zip
mBa1d905a11 mutant 7 43B a1d905a11_slices_4-5-6-7.zip
mA37e0450ac mutant 1 12A 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 2 52A 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 3 55B 37e0450ac_slices_1-2-3-x.zip
mA37e0450ac mutant 4 58A 37e0450ac_slices_4-5-6-7.zip
mAf715d5511 mutant 2 52A f715d5511_slices_1-2-3-x.zip
mAf715d5511 mutant 3 12B f715d5511_slices_1-2-3-x.zip
mB3de8f2e17 mutant 6 55B 3de8f2e17_slices_4-5-6-7.zip
mB3de8f2e17 mutant 7 61B 3de8f2e17_slices_4-5-6-7.zip
mA997de7e37 mutant 1 46A 997de7e37_slices_1-2-3-x.zip
mA997de7e37 mutant 2 42A 997de7e37_slices_1-2-3-x.zip
mA997de7e37 mutant 3 15B 997de7e37_slices_1-2-3-x.zip
mB997de7e37 mutant 4 55A 997de7e37_slices_4-5-6-x.zip
mB997de7e37 mutant 5 54A 997de7e37_slices_4-5-6-x.zip
mB997de7e37 mutant 6 46B 997de7e37_slices_4-5-6-x.zip
mAcdc2f1817 mutant 1 46A cdc2f1817_slices_1-2-3-x.zip
mAcdc2f1817 mutant 2 52A cdc2f1817_slices_1-2-3-x.zip
mAcdc2f1817 mutant 3 15B cdc2f1817_slices_1-2-3-x.zip
wB9023e5745 WT 2 52A 9023e5745_slices_1-2-3-x.zip
wB9023e5745 WT 5 52A 9023e5745_slices_4-5-6-7.zip
wB9023e5745 WT 6 15B 9023e5745_slices_4-5-6-7.zip
wB9023e5745 WT 7 54B 9023e5745_slices_4-5-6-7.zip
wAa1d905a11 WT 1 12A a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 2 52A a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 3 15B a1d905a11_slices_1-2-3-x.zip
wAa1d905a11 WT 4 38A a1d905a11_slices_4-5-6-7.zip
wB37e0450ac WT 5 61A 37e0450ac_slices_4-5-6-7.zip
wB37e0450ac WT 6 46B 37e0450ac_slices_4-5-6-7.zip
wB37e0450ac WT 7 52B 37e0450ac_slices_4-5-6-7.zip
wBf715d5511 WT 5 42A f715d5511_slices_4-5-6-7.zip
wBf715d5511 WT 7 52B f715d5511_slices_4-5-6-7.zip
wA3de8f2e17 WT 2 61A 3de8f2e17_slices_1-2-3-x.zip
wA3de8f2e17 WT 3 55B 3de8f2e17_slices_1-2-3-x.zip
wA3de8f2e17 WT 4 12A 3de8f2e17_slices_4-5-6-7.zip
wBcdc2f1817 WT 4 58A cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 5 61A cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 6 46B cdc2f1817_slices_4-5-6-7.zip
wBcdc2f1817 WT 7 43B cdc2f1817_slices_4-5-6-7.zip
wAe40eff646 WT 1 12A e40eff646_slices_1-2-3-x.zip
wAe40eff646 WT 2 41A e40eff646_slices_1-2-3-x.zip
wAe40eff646 WT 3 12B e40eff646_slices_1-2-3-x.zip
wBe40eff646 WT 4 38A e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 5 54A e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 6 55B e40eff646_slices_4-5-6-7.zip
wBe40eff646 WT 7 62B e40eff646_slices_4-5-6-7.zip

Data Files

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f715d5511_slices_1-2-3-x.zip39901169a590e6ddc585051dcbaaee4ce51e34d62e86474d68ca11a50b747d7569821b35
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