G2C::Electrophysiology

alpha-CaMKII knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed extreme overall electrophysiological difference from wildtype slices, with seven of nine variables significantly impacted by this mutation. In the basal synaptic transmission experiment, max fEPSP amplitude was significantly decreased in mutants. In the paired pulse facilitation experiment, PPF at 50ms ampl ratio was significantly decreased in mutants. In the theta burst stimulation experiment, three electrophysiological variables of five were significantly impacted in mutants. In the long term potentiation experiment, two electrophysiological variables of two were significantly impacted in mutants. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the alpha-CaMKII knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3090 (120) 15 (5) 2380 (140) 0.0049 ** 0.036 *
PPF at 50ms, ampl ratio % 17 (5) 169.7 (4.4) 15 (5) 138.7 (2.5) 0.21 0.0012 **
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 158.3 (5.2) 14 (5) 98.3 (4.8) 0.36 8.6e-05 ***
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 59.7 (1.1) 14 (5) 90.7 (3.5) 0.19 8.7e-05 ***
Burst 1, ave amplitude µV 17 (5) 1375 (51) 14 (5) 1150 (61) 0.0031 ** 0.1
Burst 2, ave amplitude µV 17 (5) 2236 (93) 14 (5) 1886 (112) 0.0013 ** 0.17
Burst 3, ave amplitude µV 17 (5) 2413 (90) 14 (5) 2009 (117) 0.0018 ** 0.12
Burst 4, ave amplitude µV 17 (5) 2420 (87) 14 (5) 2048 (117) 0.002 ** 0.14
Burst 5, ave amplitude µV 17 (5) 2355 (85) 14 (5) 2032 (119) 0.0017 ** 0.19
Burst 6, ave amplitude µV 17 (5) 2280 (78) 14 (5) 1997 (116) 0.0013 ** 0.22
Burst 7, ave amplitude µV 17 (5) 2214 (77) 14 (5) 1960 (114) 0.0019 ** 0.26
Burst 8, ave amplitude µV 17 (5) 2150 (73) 14 (5) 1922 (110) 0.0015 ** 0.29
Burst 9, ave amplitude µV 17 (5) 2096 (72) 14 (5) 1895 (110) 0.0015 ** 0.35
Burst 10, ave amplitude µV 17 (5) 2066 (71) 14 (5) 1856 (111) 0.0016 ** 0.33
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 170.7 (4.5) 14 (5) 171.3 (2.9) 0.38 0.92
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 89.3 (1.4) 14 (5) 96.7 (1.4) 0.81 0.0023 **
LTP vs PTP, amplitude ratio % 17 (5) 83.9 (0.9) 14 (5) 78 (0.9) 0.58 0.0016 **
LTP based on amplitude % 17 (5) 171.8 (4.1) 14 (5) 126 (3) 0.26 9.1e-05 ***

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3090 (120) 15 (5) 2380 (140) 0.0049 ** 0.036 *

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 17 (5) 169.7 (4.4) 15 (5) 138.7 (2.5) 0.21 0.0012 **

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 158.3 (5.2) 14 (5) 98.3 (4.8) 0.36 8.6e-05 ***
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 59.7 (1.1) 14 (5) 90.7 (3.5) 0.19 8.7e-05 ***
Burst 1, ave amplitude µV 17 (5) 1375 (51) 14 (5) 1150 (61) 0.0031 ** 0.1
Burst 2, ave amplitude µV 17 (5) 2236 (93) 14 (5) 1886 (112) 0.0013 ** 0.17
Burst 3, ave amplitude µV 17 (5) 2413 (90) 14 (5) 2009 (117) 0.0018 ** 0.12
Burst 4, ave amplitude µV 17 (5) 2420 (87) 14 (5) 2048 (117) 0.002 ** 0.14
Burst 5, ave amplitude µV 17 (5) 2355 (85) 14 (5) 2032 (119) 0.0017 ** 0.19
Burst 6, ave amplitude µV 17 (5) 2280 (78) 14 (5) 1997 (116) 0.0013 ** 0.22
Burst 7, ave amplitude µV 17 (5) 2214 (77) 14 (5) 1960 (114) 0.0019 ** 0.26
Burst 8, ave amplitude µV 17 (5) 2150 (73) 14 (5) 1922 (110) 0.0015 ** 0.29
Burst 9, ave amplitude µV 17 (5) 2096 (72) 14 (5) 1895 (110) 0.0015 ** 0.35
Burst 10, ave amplitude µV 17 (5) 2066 (71) 14 (5) 1856 (111) 0.0016 ** 0.33
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 170.7 (4.5) 14 (5) 171.3 (2.9) 0.38 0.92
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 89.3 (1.4) 14 (5) 96.7 (1.4) 0.81 0.0023 **

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 17 (5) 83.9 (0.9) 14 (5) 78 (0.9) 0.58 0.0016 **
LTP based on amplitude % 17 (5) 171.8 (4.1) 14 (5) 126 (3) 0.26 9.1e-05 ***

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wA884273babWT
wA0c3ef33f5WT
wB0c3ef33f5WT
wA08d9b3b0fWT
wAaa0d099eaWT
mBc16e1326cmutant
mAc16e1326cmutant
mBaa0d099eamutant
mB08d9b3b0fmutant
mB884273babmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBaa0d099ea mutant 4 12A aa0d099ea_slices_4-5-x-6.zip
mBaa0d099ea mutant 6 52B aa0d099ea_slices_4-5-x-6.zip
mAc16e1326c mutant 1 46A c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 2 62A c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 3 46B c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 4 55A c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 5 52A c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 6 12B c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 7 61B c16e1326c_slices_4-5-6-7.zip
mB884273bab mutant 4 47A 884273bab_slices_4-5-6-7.zip
mB884273bab mutant 5 52A 884273bab_slices_4-5-6-7.zip
mB884273bab mutant 6 58B 884273bab_slices_4-5-6-7.zip
mB08d9b3b0f mutant 4 46A 08d9b3b0f_slices_4-5-6-x.zip
mB08d9b3b0f mutant 5 54A 08d9b3b0f_slices_4-5-6-x.zip
mB08d9b3b0f mutant 6 58B 08d9b3b0f_slices_4-5-6-x.zip
wAaa0d099ea WT 1 15A aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 2 52A aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 3 58B aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 5 52A aa0d099ea_slices_4-5-x-6.zip
wA0c3ef33f5 WT 1 55A 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 2 42A 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 3 46B 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 4 58A 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 5 41A 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 6 58B 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 7 54B 0c3ef33f5_slices_4-5-6-7.zip
wA884273bab WT 1 46A 884273bab_slices_1-2-3-x.zip
wA884273bab WT 2 42A 884273bab_slices_1-2-3-x.zip
wA884273bab WT 3 55B 884273bab_slices_1-2-3-x.zip
wA884273bab WT 7 41B 884273bab_slices_4-5-6-7.zip
wA08d9b3b0f WT 1 46A 08d9b3b0f_slices_1-2-3-x.zip
wA08d9b3b0f WT 2 62A 08d9b3b0f_slices_1-2-3-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBaa0d099ea mutant 4 12A aa0d099ea_slices_4-5-x-6.zip
mBaa0d099ea mutant 6 52B aa0d099ea_slices_4-5-x-6.zip
mAc16e1326c mutant 1 46A c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 2 62A c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 3 46B c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 4 55A c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 5 52A c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 6 12B c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 7 61B c16e1326c_slices_4-5-6-7.zip
mB884273bab mutant 4 47A 884273bab_slices_4-5-6-7.zip
mB884273bab mutant 5 52A 884273bab_slices_4-5-6-7.zip
mB884273bab mutant 6 58B 884273bab_slices_4-5-6-7.zip
mB08d9b3b0f mutant 4 46A 08d9b3b0f_slices_4-5-6-x.zip
mB08d9b3b0f mutant 5 54A 08d9b3b0f_slices_4-5-6-x.zip
mB08d9b3b0f mutant 6 58B 08d9b3b0f_slices_4-5-6-x.zip
wAaa0d099ea WT 1 15A aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 2 52A aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 3 58B aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 5 52A aa0d099ea_slices_4-5-x-6.zip
wA0c3ef33f5 WT 1 55A 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 2 42A 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 3 46B 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 4 58A 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 5 41A 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 6 58B 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 7 54B 0c3ef33f5_slices_4-5-6-7.zip
wA884273bab WT 1 46A 884273bab_slices_1-2-3-x.zip
wA884273bab WT 2 42A 884273bab_slices_1-2-3-x.zip
wA884273bab WT 3 55B 884273bab_slices_1-2-3-x.zip
wA884273bab WT 7 41B 884273bab_slices_4-5-6-7.zip
wA08d9b3b0f WT 1 46A 08d9b3b0f_slices_1-2-3-x.zip
wA08d9b3b0f WT 2 62A 08d9b3b0f_slices_1-2-3-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBaa0d099ea mutant 4 12A aa0d099ea_slices_4-5-x-6.zip
mBaa0d099ea mutant 6 52B aa0d099ea_slices_4-5-x-6.zip
mAc16e1326c mutant 1 46A c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 2 62A c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 3 46B c16e1326c_slices_1-2-3-x.zip
mAc16e1326c mutant 4 55A c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 5 52A c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 6 12B c16e1326c_slices_4-5-6-7.zip
mBc16e1326c mutant 7 61B c16e1326c_slices_4-5-6-7.zip
mB884273bab mutant 4 47A 884273bab_slices_4-5-6-7.zip
mB884273bab mutant 5 52A 884273bab_slices_4-5-6-7.zip
mB08d9b3b0f mutant 4 46A 08d9b3b0f_slices_4-5-6-x.zip
mB08d9b3b0f mutant 5 54A 08d9b3b0f_slices_4-5-6-x.zip
mB08d9b3b0f mutant 6 58B 08d9b3b0f_slices_4-5-6-x.zip
wAaa0d099ea WT 1 15A aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 2 52A aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 3 58B aa0d099ea_slices_1-2-3-x.zip
wAaa0d099ea WT 5 52A aa0d099ea_slices_4-5-x-6.zip
wA0c3ef33f5 WT 1 55A 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 2 42A 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 3 46B 0c3ef33f5_slices_1-2-3-x.zip
wA0c3ef33f5 WT 4 58A 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 5 41A 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 6 58B 0c3ef33f5_slices_4-5-6-7.zip
wB0c3ef33f5 WT 7 54B 0c3ef33f5_slices_4-5-6-7.zip
wA884273bab WT 1 46A 884273bab_slices_1-2-3-x.zip
wA884273bab WT 2 42A 884273bab_slices_1-2-3-x.zip
wA884273bab WT 3 55B 884273bab_slices_1-2-3-x.zip
wA884273bab WT 7 41B 884273bab_slices_4-5-6-7.zip
wA08d9b3b0f WT 1 46A 08d9b3b0f_slices_1-2-3-x.zip
wA08d9b3b0f WT 2 62A 08d9b3b0f_slices_1-2-3-x.zip

Data Files

File NameSizeChecksum (SHA256)
884273bab_slices_4-5-6-7.zip42928302f79637c86bcef93ddfb15e425865e25755918c0b30279084825daaa0b90ef05c
aa0d099ea_slices_1-2-3-x.zip38261877c5f93e5d4c119a50cd81aa225c5fe7ad1cf57dbeaa3a6257ca03b0f87092fb68
0c3ef33f5_slices_1-2-3-x.zip36217274b492190e281833a0bc09c7edfcfca9460b39dc8929c1365e59481bdae2d37827
884273bab_slices_1-2-3-x.zip32087283408e2faddb26e0fc93637a2ee8ec20c1349def78aec79d60e8760d7d11e613ef
aa0d099ea_slices_4-5-x-6.zip4810413042a526a35e60e035d235e85cfae7f651a6e74be7bee9f5a5fec06ef01bcb7e5f
c16e1326c_slices_1-2-3-x.zip4465603420c5965834b9a37841d95df5ecef4558919ce063fcb45f301b48e2598324fd43
c16e1326c_slices_4-5-6-7.zip453927745481681ae2af794e6f070d9e6136701a98cde8ad969e2a3a45d6fe2fd689b90d
08d9b3b0f_slices_4-5-6-x.zip5221834084fcfb053edfc4abdcdadd3e6c265aeed4eef70855bc79664e257e4ab1aa5cc4
08d9b3b0f_slices_1-2-3-x.zip42575090119cef69a202adc027b46b29d29eaaa0deaeaa1500890b8f61e4eea75cf96c3c
0c3ef33f5_slices_4-5-6-7.zip4935828036d86e00c68494d29904b70549853ff87867c6110718f0337ef0e1aaa205b601
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