G2C::Electrophysiology

Cortactin knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices. With heterozygous genotype and no electrophysiological variables significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Cortactin knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 21 (6) 3220 (110) 19 (7) 2990 (180) 0.0018 ** 0.47
PPF at 50ms, ampl ratio % 21 (6) 176.3 (3.8) 19 (7) 178.2 (2.9) 0.073 0.75
Burst 1, PPF at 10ms, ampl ratio % 20 (6) 194.9 (6) 18 (7) 209.6 (5.2) 0.21 0.14
Burst 1, EPSP3 depr, ampl ratio % 20 (6) 59.6 (1.9) 18 (7) 62.7 (3) 0.23 0.45
Burst 1, ave amplitude µV 20 (6) 1299 (38) 18 (7) 1195 (56) 0.037 * 0.26
Burst 2, ave amplitude µV 20 (6) 2085 (85) 18 (7) 1789 (104) 0.015 * 0.14
Burst 3, ave amplitude µV 20 (6) 2340 (94) 18 (7) 2029 (123) 0.003 ** 0.2
Burst 4, ave amplitude µV 20 (6) 2402 (88) 18 (7) 2167 (120) 0.0036 ** 0.3
Burst 5, ave amplitude µV 20 (6) 2350 (84) 18 (7) 2155 (114) 0.0054 ** 0.35
Burst 6, ave amplitude µV 20 (6) 2292 (81) 18 (7) 2110 (109) 0.01 * 0.36
Burst 7, ave amplitude µV 20 (6) 2218 (80) 18 (7) 2052 (104) 0.0091 ** 0.38
Burst 8, ave amplitude µV 20 (6) 2142 (82) 18 (7) 1993 (103) 0.011 * 0.43
Burst 9, ave amplitude µV 20 (6) 2091 (85) 18 (7) 1959 (103) 0.01 * 0.5
Burst 10, ave amplitude µV 20 (6) 2029 (84) 18 (7) 1907 (101) 0.0084 ** 0.52
Burst 2-4 ave amplitude vs Burst 1 % 20 (6) 175.1 (3.7) 18 (7) 165.9 (4.3) 0.072 0.23
Burst 8-10 ave amplitude vs Burst 2-4 % 20 (6) 92.2 (1.1) 18 (7) 99.9 (2.2) 0.00057 *** 0.055
LTP vs PTP, amplitude ratio % 20 (6) 78.1 (1) 18 (7) 75.9 (1.3) 0.18 0.28
LTP based on amplitude % 20 (6) 178.1 (3.9) 18 (7) 170.2 (7.8) 2.9e-05 *** 0.58

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 21 (6) 3220 (110) 19 (7) 2990 (180) 0.0018 ** 0.47

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 21 (6) 176.3 (3.8) 19 (7) 178.2 (2.9) 0.073 0.75

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 20 (6) 194.9 (6) 18 (7) 209.6 (5.2) 0.21 0.14
Burst 1, EPSP3 depr, ampl ratio % 20 (6) 59.6 (1.9) 18 (7) 62.7 (3) 0.23 0.45
Burst 1, ave amplitude µV 20 (6) 1299 (38) 18 (7) 1195 (56) 0.037 * 0.26
Burst 2, ave amplitude µV 20 (6) 2085 (85) 18 (7) 1789 (104) 0.015 * 0.14
Burst 3, ave amplitude µV 20 (6) 2340 (94) 18 (7) 2029 (123) 0.003 ** 0.2
Burst 4, ave amplitude µV 20 (6) 2402 (88) 18 (7) 2167 (120) 0.0036 ** 0.3
Burst 5, ave amplitude µV 20 (6) 2350 (84) 18 (7) 2155 (114) 0.0054 ** 0.35
Burst 6, ave amplitude µV 20 (6) 2292 (81) 18 (7) 2110 (109) 0.01 * 0.36
Burst 7, ave amplitude µV 20 (6) 2218 (80) 18 (7) 2052 (104) 0.0091 ** 0.38
Burst 8, ave amplitude µV 20 (6) 2142 (82) 18 (7) 1993 (103) 0.011 * 0.43
Burst 9, ave amplitude µV 20 (6) 2091 (85) 18 (7) 1959 (103) 0.01 * 0.5
Burst 10, ave amplitude µV 20 (6) 2029 (84) 18 (7) 1907 (101) 0.0084 ** 0.52
Burst 2-4 ave amplitude vs Burst 1 % 20 (6) 175.1 (3.7) 18 (7) 165.9 (4.3) 0.072 0.23
Burst 8-10 ave amplitude vs Burst 2-4 % 20 (6) 92.2 (1.1) 18 (7) 99.9 (2.2) 0.00057 *** 0.055

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 20 (6) 78.1 (1) 18 (7) 75.9 (1.3) 0.18 0.28
LTP based on amplitude % 20 (6) 178.1 (3.9) 18 (7) 170.2 (7.8) 2.9e-05 *** 0.58

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT7
mutant7

 

Individual Mice

Mouse IDGenotype
wAe0a98912eWT
wBe9103c0edWT
wAdab43a26dWT
wAe9ac76fe7WT
wBe0a98912eWT
wBf6cfe2816WT
wAe9103c0edWT
mA3c599ab71mutant
mB3c599ab71mutant
mBdab43a26dmutant
mBe9ac76fe7mutant
mAf6cfe2816mutant
mB2541eded9mutant
mA2541eded9mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA3c599ab71 mutant 1 46A 3c599ab71_slice_1-2-3-x.zip
mA3c599ab71 mutant 3 12B 3c599ab71_slice_1-2-3-x.zip
mA3c599ab71 mutant 4 12A 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 5 52A 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 6 15B 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 7 41B 3c599ab71_slices_4-5-6-7.zip
mAf6cfe2816 mutant 1 46A f6cfe2816_slice_1-2-3-x.zip
mAf6cfe2816 mutant 2 42A f6cfe2816_slice_1-2-3-x.zip
mAf6cfe2816 mutant 3 12B f6cfe2816_slice_1-2-3-x.zip
mBdab43a26d mutant 4 58A dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 5 41A dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 6 58B dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 7 52B dab43a26d_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 5 62A e9ac76fe7_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 6 55B e9ac76fe7_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 7 54B e9ac76fe7_slices_4-5-6-7.zip
mB2541eded9 mutant 1 12A 2541eded9_slices_1-2-3-x.zip
mA2541eded9 mutant 2 52A 2541eded9_slices_1-2-3-x.zip
mB2541eded9 mutant 4 57A 2541eded9_slices_4-5-6-7.zip
mA2541eded9 mutant 5 52A 2541eded9_slices_4-5-6-7.zip
mA2541eded9 mutant 6 46B 2541eded9_slices_4-5-6-7.zip
wAe9103c0ed WT 1 55A e9103c0ed_slice_1-2-3-x.zip
wAe9103c0ed WT 2 41A e9103c0ed_slice_1-2-3-x.zip
wAe9103c0ed WT 3 46B e9103c0ed_slice_1-2-3-x.zip
wBe9103c0ed WT 4 58A Not available
wBe9103c0ed WT 5 52A Not available
wBe9103c0ed WT 6 55B Not available
wBe9103c0ed WT 7 54B Not available
wBf6cfe2816 WT 4 12A f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 5 54A f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 6 15B f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 7 52B f6cfe2816_slices_4-5-6-7.zip
wAdab43a26d WT 1 46A dab43a26d_slice_1-2-3-x.zip
wAdab43a26d WT 2 52A dab43a26d_slice_1-2-3-x.zip
wAdab43a26d WT 3 38B dab43a26d_slice_1-2-3-x.zip
wAe9ac76fe7 WT 1 46A e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 2 61A e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 3 46B e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 4 58A e9ac76fe7_slices_4-5-6-7.zip
wAe0a98912e WT 1 46A e0a98912e_slices_1-2-3-x.zip
wAe0a98912e WT 2 52A e0a98912e_slices_1-2-3-x.zip
wAe0a98912e WT 3 15B e0a98912e_slices_1-2-3-x.zip
wBe0a98912e WT 4 55A e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 5 52A e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 6 46B e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 7 52B e0a98912e_slices_4-5-6-7.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA3c599ab71 mutant 1 46A 3c599ab71_slice_1-2-3-x.zip
mA3c599ab71 mutant 3 12B 3c599ab71_slice_1-2-3-x.zip
mA3c599ab71 mutant 4 12A 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 5 52A 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 6 15B 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 7 41B 3c599ab71_slices_4-5-6-7.zip
mAf6cfe2816 mutant 1 46A f6cfe2816_slice_1-2-3-x.zip
mAf6cfe2816 mutant 2 42A f6cfe2816_slice_1-2-3-x.zip
mAf6cfe2816 mutant 3 12B f6cfe2816_slice_1-2-3-x.zip
mBdab43a26d mutant 4 58A dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 5 41A dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 6 58B dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 7 52B dab43a26d_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 5 62A e9ac76fe7_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 6 55B e9ac76fe7_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 7 54B e9ac76fe7_slices_4-5-6-7.zip
mB2541eded9 mutant 1 12A 2541eded9_slices_1-2-3-x.zip
mA2541eded9 mutant 2 52A 2541eded9_slices_1-2-3-x.zip
mB2541eded9 mutant 4 57A 2541eded9_slices_4-5-6-7.zip
mA2541eded9 mutant 5 52A 2541eded9_slices_4-5-6-7.zip
mA2541eded9 mutant 6 46B 2541eded9_slices_4-5-6-7.zip
wAe9103c0ed WT 1 55A e9103c0ed_slice_1-2-3-x.zip
wAe9103c0ed WT 2 41A e9103c0ed_slice_1-2-3-x.zip
wAe9103c0ed WT 3 46B e9103c0ed_slice_1-2-3-x.zip
wBe9103c0ed WT 4 58A Not available
wBe9103c0ed WT 5 52A Not available
wBe9103c0ed WT 6 55B Not available
wBe9103c0ed WT 7 54B Not available
wBf6cfe2816 WT 4 12A f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 5 54A f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 6 15B f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 7 52B f6cfe2816_slices_4-5-6-7.zip
wAdab43a26d WT 1 46A dab43a26d_slice_1-2-3-x.zip
wAdab43a26d WT 2 52A dab43a26d_slice_1-2-3-x.zip
wAdab43a26d WT 3 38B dab43a26d_slice_1-2-3-x.zip
wAe9ac76fe7 WT 1 46A e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 2 61A e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 3 46B e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 4 58A e9ac76fe7_slices_4-5-6-7.zip
wAe0a98912e WT 1 46A e0a98912e_slices_1-2-3-x.zip
wAe0a98912e WT 2 52A e0a98912e_slices_1-2-3-x.zip
wAe0a98912e WT 3 15B e0a98912e_slices_1-2-3-x.zip
wBe0a98912e WT 4 55A e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 5 52A e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 6 46B e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 7 52B e0a98912e_slices_4-5-6-7.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA3c599ab71 mutant 1 46A 3c599ab71_slice_1-2-3-x.zip
mA3c599ab71 mutant 3 12B 3c599ab71_slice_1-2-3-x.zip
mA3c599ab71 mutant 4 12A 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 5 52A 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 6 15B 3c599ab71_slices_4-5-6-7.zip
mB3c599ab71 mutant 7 41B 3c599ab71_slices_4-5-6-7.zip
mAf6cfe2816 mutant 1 46A f6cfe2816_slice_1-2-3-x.zip
mAf6cfe2816 mutant 2 42A f6cfe2816_slice_1-2-3-x.zip
mAf6cfe2816 mutant 3 12B f6cfe2816_slice_1-2-3-x.zip
mBdab43a26d mutant 5 41A dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 6 58B dab43a26d_slices_4-5-6-7.zip
mBdab43a26d mutant 7 52B dab43a26d_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 5 62A e9ac76fe7_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 6 55B e9ac76fe7_slices_4-5-6-7.zip
mBe9ac76fe7 mutant 7 54B e9ac76fe7_slices_4-5-6-7.zip
mB2541eded9 mutant 1 12A 2541eded9_slices_1-2-3-x.zip
mA2541eded9 mutant 2 52A 2541eded9_slices_1-2-3-x.zip
mB2541eded9 mutant 4 57A 2541eded9_slices_4-5-6-7.zip
mA2541eded9 mutant 5 52A 2541eded9_slices_4-5-6-7.zip
mA2541eded9 mutant 6 46B 2541eded9_slices_4-5-6-7.zip
wAe9103c0ed WT 1 55A e9103c0ed_slice_1-2-3-x.zip
wAe9103c0ed WT 2 41A e9103c0ed_slice_1-2-3-x.zip
wAe9103c0ed WT 3 46B e9103c0ed_slice_1-2-3-x.zip
wBe9103c0ed WT 4 58A Not available
wBe9103c0ed WT 5 52A Not available
wBe9103c0ed WT 6 55B Not available
wBe9103c0ed WT 7 54B Not available
wBf6cfe2816 WT 4 12A f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 5 54A f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 6 15B f6cfe2816_slices_4-5-6-7.zip
wBf6cfe2816 WT 7 52B f6cfe2816_slices_4-5-6-7.zip
wAdab43a26d WT 1 46A dab43a26d_slice_1-2-3-x.zip
wAdab43a26d WT 2 52A dab43a26d_slice_1-2-3-x.zip
wAdab43a26d WT 3 38B dab43a26d_slice_1-2-3-x.zip
wAe9ac76fe7 WT 1 46A e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 2 61A e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 3 46B e9ac76fe7_slice_1-2-3-x.zip
wAe9ac76fe7 WT 4 58A e9ac76fe7_slices_4-5-6-7.zip
wAe0a98912e WT 1 46A e0a98912e_slices_1-2-3-x.zip
wAe0a98912e WT 2 52A e0a98912e_slices_1-2-3-x.zip
wBe0a98912e WT 4 55A e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 5 52A e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 6 46B e0a98912e_slices_4-5-6-7.zip
wBe0a98912e WT 7 52B e0a98912e_slices_4-5-6-7.zip

Data Files

File NameSizeChecksum (SHA256)
3c599ab71_slices_4-5-6-7.zip4550289358fed1fa85737d6d9246159f7005f4f703c70d9ab4624cce3e53a20bab7e3e22
f6cfe2816_slices_4-5-6-7.zip45465326268059a34df0a55e93e34506c76379de142c53e14fe635b165a50e3459de5a57
e9103c0ed_slice_1-2-3-x.zip37567611a2b83c8ae8bf0298f78670788c75cae321b1118391be1d676b403e66fcc9147b
e9ac76fe7_slices_4-5-6-7.zip56732447b93ab25811b82cf131c860df2369781931503c80bbc539019fd4877d8e4d5e8a
dab43a26d_slices_4-5-6-7.zip54133243e5c4ada1a83a1261632dd7481611883a60c4449ddfe5d808308b99be4d6c0ade
e9ac76fe7_slice_1-2-3-x.zip43829748c33457c6b6a52617a93c391fd30268d4cf0747e05ae96edbf4ac37fdfe5e76c9
e0a98912e_slices_4-5-6-7.zip465287245bc470b2b47df6c3227a777ba853a58f341e34cb73043591711ea6b8db745861
f6cfe2816_slice_1-2-3-x.zip36777940965659ce3c7e6ec58118ca473c12f28b5f39258e66da16f8b8d3807694e97f6c
2541eded9_slices_4-5-6-7.zip6973603197897e5d233b539ce24ba70ab3eba1b458be625ae95f7734ab0d0be5e44c684e
3c599ab71_slice_1-2-3-x.zip3175425760f0d68885e2e8b635f06ab2b4d03e8c157f143699044d30de3e0d0ddab2aefe
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e0a98912e_slices_1-2-3-x.zip3855525778dc7b4d460e0a150089b7a85b0e69a0648057910059441624eacf9353f9734b
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EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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