G2C::Electrophysiology

MUPP1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices. With heterozygous genotype and only one electrophysiological variable significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the MUPP1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 15 (5) 3100 (160) 29 (9) 3190 (110) 0.74 0.59
PPF at 50ms, ampl ratio % 15 (5) 186.8 (5.8) 29 (9) 176.7 (4.3) 0.17 0.26
Burst 1, PPF at 10ms, ampl ratio % 15 (5) 213.1 (8.8) 29 (9) 184.1 (5.2) 0.012 * 0.048 *
Burst 1, EPSP3 depr, ampl ratio % 15 (5) 60 (4.4) 29 (9) 63.2 (2.8) 0.022 * 0.64
Burst 1, ave amplitude µV 15 (5) 1292 (65) 29 (9) 1339 (42) 0.81 0.46
Burst 2, ave amplitude µV 15 (5) 2003 (118) 29 (9) 2059 (70) 0.83 0.6
Burst 3, ave amplitude µV 15 (5) 2281 (144) 29 (9) 2326 (91) 0.8 0.74
Burst 4, ave amplitude µV 15 (5) 2389 (151) 29 (9) 2370 (91) 0.83 0.89
Burst 5, ave amplitude µV 15 (5) 2362 (146) 28 (9) 2340 (93) 0.84 0.87
Burst 6, ave amplitude µV 15 (5) 2279 (143) 28 (9) 2264 (91) 0.9 0.9
Burst 7, ave amplitude µV 15 (5) 2206 (139) 28 (9) 2178 (89) 0.91 0.82
Burst 8, ave amplitude µV 15 (5) 2128 (134) 28 (9) 2101 (88) 0.88 0.83
Burst 9, ave amplitude µV 15 (5) 2069 (132) 28 (9) 2038 (87) 0.86 0.8
Burst 10, ave amplitude µV 15 (5) 2023 (129) 28 (9) 2000 (86) 0.86 0.85
Burst 2-4 ave amplitude vs Burst 1 % 15 (5) 170.5 (5.5) 29 (9) 167.5 (3.8) 0.85 0.58
Burst 8-10 ave amplitude vs Burst 2-4 % 15 (5) 93.9 (2.1) 28 (9) 90.7 (1.2) 0.17 0.24
LTP vs PTP, amplitude ratio % 15 (5) 80.1 (1.6) 29 (9) 82.2 (1.1) 0.039 * 0.39
LTP based on amplitude % 15 (5) 183.5 (6.1) 29 (9) 164.7 (5) 0.23 0.067

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 15 (5) 3100 (160) 29 (9) 3190 (110) 0.74 0.59

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 15 (5) 186.8 (5.8) 29 (9) 176.7 (4.3) 0.17 0.26

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 15 (5) 213.1 (8.8) 29 (9) 184.1 (5.2) 0.012 * 0.048 *
Burst 1, EPSP3 depr, ampl ratio % 15 (5) 60 (4.4) 29 (9) 63.2 (2.8) 0.022 * 0.64
Burst 1, ave amplitude µV 15 (5) 1292 (65) 29 (9) 1339 (42) 0.81 0.46
Burst 2, ave amplitude µV 15 (5) 2003 (118) 29 (9) 2059 (70) 0.83 0.6
Burst 3, ave amplitude µV 15 (5) 2281 (144) 29 (9) 2326 (91) 0.8 0.74
Burst 4, ave amplitude µV 15 (5) 2389 (151) 29 (9) 2370 (91) 0.83 0.89
Burst 5, ave amplitude µV 15 (5) 2362 (146) 28 (9) 2340 (93) 0.84 0.87
Burst 6, ave amplitude µV 15 (5) 2279 (143) 28 (9) 2264 (91) 0.9 0.9
Burst 7, ave amplitude µV 15 (5) 2206 (139) 28 (9) 2178 (89) 0.91 0.82
Burst 8, ave amplitude µV 15 (5) 2128 (134) 28 (9) 2101 (88) 0.88 0.83
Burst 9, ave amplitude µV 15 (5) 2069 (132) 28 (9) 2038 (87) 0.86 0.8
Burst 10, ave amplitude µV 15 (5) 2023 (129) 28 (9) 2000 (86) 0.86 0.85
Burst 2-4 ave amplitude vs Burst 1 % 15 (5) 170.5 (5.5) 29 (9) 167.5 (3.8) 0.85 0.58
Burst 8-10 ave amplitude vs Burst 2-4 % 15 (5) 93.9 (2.1) 28 (9) 90.7 (1.2) 0.17 0.24

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 15 (5) 80.1 (1.6) 29 (9) 82.2 (1.1) 0.039 * 0.39
LTP based on amplitude % 15 (5) 183.5 (6.1) 29 (9) 164.7 (5) 0.23 0.067

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant10

 

Individual Mice

Mouse IDGenotype
wA40960df6cWT
wA92a24f5e6WT
wA9fa4bf41bWT
wA4921fc417WT
wA21ada41aaWT
mA172c8540emutant
mAa24986c40mutant
mAde750c5edmutant
mAaf1c52975mutant
mA866fd18f5mutant
mA19824c905mutant
mAea02bf639mutant
mA9c8d8ccacmutant
mAed36876eamutant
mA8f53eedcemutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA172c8540e mutant 1 31A 172c8540e_slices_1-x-2-3.zip
mA172c8540e mutant 2 58B 172c8540e_slices_1-x-2-3.zip
mA172c8540e mutant 3 61B 172c8540e_slices_1-x-2-3.zip
mAa24986c40 mutant 1 67A a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 2 52A a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 3 55B a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 4 52B a24986c40_slices_1-2-3-4.zip
mA866fd18f5 mutant 2 42A 866fd18f5_slices_1-2-3-4.zip
mA866fd18f5 mutant 3 12B 866fd18f5_slices_1-2-3-4.zip
mA866fd18f5 mutant 4 52B 866fd18f5_slices_1-2-3-4.zip
mAea02bf639 mutant 2 52A ea02bf639_slices_1-2-3-4.zip
mAea02bf639 mutant 3 38B ea02bf639_slices_1-2-3-4.zip
mAea02bf639 mutant 4 71B ea02bf639_slices_1-2-3-4.zip
mA8f53eedce mutant 1 61A 8f53eedce_slices_x-1-2-3.zip
mA8f53eedce mutant 2 47B 8f53eedce_slices_x-1-2-3.zip
mA8f53eedce mutant 3 42B 8f53eedce_slices_x-1-2-3.zip
mAde750c5ed mutant 2 73A de750c5ed_slices_1-2-3-4.zip
mAde750c5ed mutant 3 55B de750c5ed_slices_1-2-3-4.zip
mAde750c5ed mutant 4 62B de750c5ed_slices_1-2-3-4.zip
mA9c8d8ccac mutant 2 75A 9c8d8ccac_slices_1-2-3-4.zip
mA9c8d8ccac mutant 3 15B 9c8d8ccac_slices_1-2-3-4.zip
mA9c8d8ccac mutant 4 43B 9c8d8ccac_slices_1-2-3-4.zip
mAed36876ea mutant 1 78A ed36876ea_slices_1-x-2-3.zip
mAed36876ea mutant 2 55B ed36876ea_slices_1-x-2-3.zip
mAed36876ea mutant 3 84B ed36876ea_slices_1-x-2-3.zip
mA19824c905 mutant 1 55A 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 2 61A 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 3 67B 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 4 62B 19824c905_slices_1-2-3-4.zip
mAaf1c52975 mutant 2 84A af1c52975_slices_1-2-3-4.zip
mAaf1c52975 mutant 3 55B af1c52975_slices_1-2-3-4.zip
mAaf1c52975 mutant 4 62B af1c52975_slices_1-2-3-4.zip
wA92a24f5e6 WT 1 47A 92a24f5e6_slices_1-2-3-4.zip
wA92a24f5e6 WT 2 62A 92a24f5e6_slices_1-2-3-4.zip
wA4921fc417 WT 1 58A 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 2 42A 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 3 55B 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 4 62B 4921fc417_slices_1-2-3-4.zip
wA9fa4bf41b WT 2 12B 9fa4bf41b_slices_x-1-2-3.zip
wA9fa4bf41b WT 3 43B 9fa4bf41b_slices_x-1-2-3.zip
wA40960df6c WT 1 47A 40960df6c_slices_1-2-3-x.zip
wA40960df6c WT 2 41A 40960df6c_slices_1-2-3-x.zip
wA40960df6c WT 3 56B 40960df6c_slices_1-2-3-x.zip
wA21ada41aa WT 1 46A 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 2 53A 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 3 57B 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 4 52B 21ada41aa_slices_1-2-3-4.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA172c8540e mutant 1 31A 172c8540e_slices_1-x-2-3.zip
mA172c8540e mutant 2 58B 172c8540e_slices_1-x-2-3.zip
mA172c8540e mutant 3 61B 172c8540e_slices_1-x-2-3.zip
mAa24986c40 mutant 1 67A a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 2 52A a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 3 55B a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 4 52B a24986c40_slices_1-2-3-4.zip
mA866fd18f5 mutant 2 42A 866fd18f5_slices_1-2-3-4.zip
mA866fd18f5 mutant 3 12B 866fd18f5_slices_1-2-3-4.zip
mA866fd18f5 mutant 4 52B 866fd18f5_slices_1-2-3-4.zip
mAea02bf639 mutant 2 52A ea02bf639_slices_1-2-3-4.zip
mAea02bf639 mutant 3 38B ea02bf639_slices_1-2-3-4.zip
mAea02bf639 mutant 4 71B ea02bf639_slices_1-2-3-4.zip
mA8f53eedce mutant 1 61A 8f53eedce_slices_x-1-2-3.zip
mA8f53eedce mutant 2 47B 8f53eedce_slices_x-1-2-3.zip
mA8f53eedce mutant 3 42B 8f53eedce_slices_x-1-2-3.zip
mAde750c5ed mutant 2 73A de750c5ed_slices_1-2-3-4.zip
mAde750c5ed mutant 3 55B de750c5ed_slices_1-2-3-4.zip
mAde750c5ed mutant 4 62B de750c5ed_slices_1-2-3-4.zip
mA9c8d8ccac mutant 2 75A 9c8d8ccac_slices_1-2-3-4.zip
mA9c8d8ccac mutant 3 15B 9c8d8ccac_slices_1-2-3-4.zip
mA9c8d8ccac mutant 4 43B 9c8d8ccac_slices_1-2-3-4.zip
mAed36876ea mutant 1 78A ed36876ea_slices_1-x-2-3.zip
mAed36876ea mutant 2 55B ed36876ea_slices_1-x-2-3.zip
mAed36876ea mutant 3 84B ed36876ea_slices_1-x-2-3.zip
mA19824c905 mutant 1 55A 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 2 61A 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 3 67B 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 4 62B 19824c905_slices_1-2-3-4.zip
mAaf1c52975 mutant 2 84A af1c52975_slices_1-2-3-4.zip
mAaf1c52975 mutant 3 55B af1c52975_slices_1-2-3-4.zip
mAaf1c52975 mutant 4 62B af1c52975_slices_1-2-3-4.zip
wA92a24f5e6 WT 1 47A 92a24f5e6_slices_1-2-3-4.zip
wA92a24f5e6 WT 2 62A 92a24f5e6_slices_1-2-3-4.zip
wA4921fc417 WT 1 58A 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 2 42A 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 3 55B 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 4 62B 4921fc417_slices_1-2-3-4.zip
wA9fa4bf41b WT 2 12B 9fa4bf41b_slices_x-1-2-3.zip
wA9fa4bf41b WT 3 43B 9fa4bf41b_slices_x-1-2-3.zip
wA40960df6c WT 1 47A 40960df6c_slices_1-2-3-x.zip
wA40960df6c WT 2 41A 40960df6c_slices_1-2-3-x.zip
wA40960df6c WT 3 56B 40960df6c_slices_1-2-3-x.zip
wA21ada41aa WT 1 46A 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 2 53A 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 3 57B 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 4 52B 21ada41aa_slices_1-2-3-4.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA172c8540e mutant 1 31A 172c8540e_slices_1-x-2-3.zip
mA172c8540e mutant 2 58B 172c8540e_slices_1-x-2-3.zip
mA172c8540e mutant 3 61B 172c8540e_slices_1-x-2-3.zip
mAa24986c40 mutant 1 67A a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 2 52A a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 3 55B a24986c40_slices_1-2-3-4.zip
mAa24986c40 mutant 4 52B a24986c40_slices_1-2-3-4.zip
mA866fd18f5 mutant 2 42A 866fd18f5_slices_1-2-3-4.zip
mA866fd18f5 mutant 3 12B 866fd18f5_slices_1-2-3-4.zip
mA866fd18f5 mutant 4 52B 866fd18f5_slices_1-2-3-4.zip
mAea02bf639 mutant 2 52A ea02bf639_slices_1-2-3-4.zip
mAea02bf639 mutant 3 38B ea02bf639_slices_1-2-3-4.zip
mAea02bf639 mutant 4 71B ea02bf639_slices_1-2-3-4.zip
mA8f53eedce mutant 1 61A 8f53eedce_slices_x-1-2-3.zip
mA8f53eedce mutant 2 47B 8f53eedce_slices_x-1-2-3.zip
mA8f53eedce mutant 3 42B 8f53eedce_slices_x-1-2-3.zip
mAde750c5ed mutant 2 73A de750c5ed_slices_1-2-3-4.zip
mAde750c5ed mutant 3 55B de750c5ed_slices_1-2-3-4.zip
mAde750c5ed mutant 4 62B de750c5ed_slices_1-2-3-4.zip
mA9c8d8ccac mutant 2 75A 9c8d8ccac_slices_1-2-3-4.zip
mA9c8d8ccac mutant 3 15B 9c8d8ccac_slices_1-2-3-4.zip
mA9c8d8ccac mutant 4 43B 9c8d8ccac_slices_1-2-3-4.zip
mAed36876ea mutant 1 78A ed36876ea_slices_1-x-2-3.zip
mAed36876ea mutant 2 55B ed36876ea_slices_1-x-2-3.zip
mAed36876ea mutant 3 84B ed36876ea_slices_1-x-2-3.zip
mA19824c905 mutant 1 55A 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 2 61A 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 3 67B 19824c905_slices_1-2-3-4.zip
mA19824c905 mutant 4 62B 19824c905_slices_1-2-3-4.zip
mAaf1c52975 mutant 2 84A af1c52975_slices_1-2-3-4.zip
mAaf1c52975 mutant 3 55B af1c52975_slices_1-2-3-4.zip
mAaf1c52975 mutant 4 62B af1c52975_slices_1-2-3-4.zip
wA92a24f5e6 WT 1 47A 92a24f5e6_slices_1-2-3-4.zip
wA92a24f5e6 WT 2 62A 92a24f5e6_slices_1-2-3-4.zip
wA4921fc417 WT 1 58A 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 2 42A 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 3 55B 4921fc417_slices_1-2-3-4.zip
wA4921fc417 WT 4 62B 4921fc417_slices_1-2-3-4.zip
wA9fa4bf41b WT 2 12B 9fa4bf41b_slices_x-1-2-3.zip
wA9fa4bf41b WT 3 43B 9fa4bf41b_slices_x-1-2-3.zip
wA40960df6c WT 1 47A 40960df6c_slices_1-2-3-x.zip
wA40960df6c WT 2 41A 40960df6c_slices_1-2-3-x.zip
wA40960df6c WT 3 56B 40960df6c_slices_1-2-3-x.zip
wA21ada41aa WT 1 46A 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 2 53A 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 3 57B 21ada41aa_slices_1-2-3-4.zip
wA21ada41aa WT 4 52B 21ada41aa_slices_1-2-3-4.zip

Data Files

File NameSizeChecksum (SHA256)
a24986c40_slices_1-2-3-4.zip41377543bcb5edc74e8eda9720512d98f8d6f0f3ddc123112607dcfc267cd4d19bb637e9
21ada41aa_slices_1-2-3-4.zip419370989acea9b40cc0c45b83b2d998a5176ae24fbdafcd5e9475f032fde2d9b0e11687
af1c52975_slices_1-2-3-4.zip4124094253b3721b5070bfdc8cf4411603620fa562e0c4625da3eb2e0cb56b575b6fa0b8
19824c905_slices_1-2-3-4.zip39938992fa84141b0fa38594d0ba2d377785d49a60dd2afebf00761da96361a544a6dcb0
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