G2C::Electrophysiology

GluR1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed large overall electrophysiological difference from wildtype slices, with five of nine variables significantly impacted in these mutant slices. In the paired pulse facilitation experiment, PPF at 50ms ampl ratio was significantly increased in mutants. In the theta burst stimulation experiment, two electrophysiological variables of five were significantly impacted in mutants. In the long term potentiation experiment, two electrophysiological variables of two were significantly impacted in mutants. Only the basal synaptic transmission experiment was unaffected. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the GluR1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 18 (5) 3330 (180) 19 (5) 2730 (110) 0.11 0.051
PPF at 50ms, ampl ratio % 18 (5) 182.3 (4.1) 19 (5) 208.8 (4.4) 0.96 0.00021 ***
Burst 1, PPF at 10ms, ampl ratio % 18 (5) 183.2 (6.3) 19 (5) 240 (5.1) 0.45 0.00016 ***
Burst 1, EPSP3 depr, ampl ratio % 18 (5) 62.3 (2.8) 19 (5) 68.4 (3.5) 0.32 0.25
Burst 1, ave amplitude µV 18 (5) 1396 (79) 19 (5) 1188 (56) 0.099 0.13
Burst 2, ave amplitude µV 18 (5) 2565 (126) 19 (5) 2036 (98) 0.23 0.021 *
Burst 3, ave amplitude µV 18 (5) 2770 (130) 19 (5) 2280 (110) 0.39 0.031 *
Burst 4, ave amplitude µV 18 (5) 2760 (130) 19 (5) 2360 (110) 0.42 0.054
Burst 5, ave amplitude µV 18 (5) 2680 (120) 19 (5) 2350 (120) 0.52 0.081
Burst 6, ave amplitude µV 18 (5) 2610 (120) 19 (5) 2290 (120) 0.51 0.091
Burst 7, ave amplitude µV 18 (5) 2550 (120) 19 (5) 2220 (120) 0.54 0.086
Burst 8, ave amplitude µV 18 (5) 2490 (120) 19 (5) 2150 (120) 0.6 0.069
Burst 9, ave amplitude µV 18 (5) 2430 (120) 19 (5) 2080 (120) 0.63 0.053
Burst 10, ave amplitude µV 18 (5) 2380 (110) 19 (5) 2010 (120) 0.64 0.041 *
Burst 2-4 ave amplitude vs Burst 1 % 18 (5) 195.8 (5.6) 19 (5) 186.1 (4.3) 0.85 0.1
Burst 8-10 ave amplitude vs Burst 2-4 % 18 (5) 91.2 (1.6) 19 (5) 93.1 (1.9) 0.48 0.48
LTP vs PTP, amplitude ratio % 18 (5) 77.9 (2.2) 19 (5) 94.9 (1.3) 0.25 0.00044 ***
LTP based on amplitude % 18 (5) 180.4 (5.4) 19 (5) 161.7 (3.4) 0.87 0.0046 **

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 18 (5) 3330 (180) 19 (5) 2730 (110) 0.11 0.051

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 18 (5) 182.3 (4.1) 19 (5) 208.8 (4.4) 0.96 0.00021 ***

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 18 (5) 183.2 (6.3) 19 (5) 240 (5.1) 0.45 0.00016 ***
Burst 1, EPSP3 depr, ampl ratio % 18 (5) 62.3 (2.8) 19 (5) 68.4 (3.5) 0.32 0.25
Burst 1, ave amplitude µV 18 (5) 1396 (79) 19 (5) 1188 (56) 0.099 0.13
Burst 2, ave amplitude µV 18 (5) 2565 (126) 19 (5) 2036 (98) 0.23 0.021 *
Burst 3, ave amplitude µV 18 (5) 2770 (130) 19 (5) 2280 (110) 0.39 0.031 *
Burst 4, ave amplitude µV 18 (5) 2760 (130) 19 (5) 2360 (110) 0.42 0.054
Burst 5, ave amplitude µV 18 (5) 2680 (120) 19 (5) 2350 (120) 0.52 0.081
Burst 6, ave amplitude µV 18 (5) 2610 (120) 19 (5) 2290 (120) 0.51 0.091
Burst 7, ave amplitude µV 18 (5) 2550 (120) 19 (5) 2220 (120) 0.54 0.086
Burst 8, ave amplitude µV 18 (5) 2490 (120) 19 (5) 2150 (120) 0.6 0.069
Burst 9, ave amplitude µV 18 (5) 2430 (120) 19 (5) 2080 (120) 0.63 0.053
Burst 10, ave amplitude µV 18 (5) 2380 (110) 19 (5) 2010 (120) 0.64 0.041 *
Burst 2-4 ave amplitude vs Burst 1 % 18 (5) 195.8 (5.6) 19 (5) 186.1 (4.3) 0.85 0.1
Burst 8-10 ave amplitude vs Burst 2-4 % 18 (5) 91.2 (1.6) 19 (5) 93.1 (1.9) 0.48 0.48

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 18 (5) 77.9 (2.2) 19 (5) 94.9 (1.3) 0.25 0.00044 ***
LTP based on amplitude % 18 (5) 180.4 (5.4) 19 (5) 161.7 (3.4) 0.87 0.0046 **

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wA353fea3aaWT
wA41f70cdb5WT
wA5745bea3bWT
wAdcbbf1abfWT
wA766917d1aWT
mAc04cc9146mutant
mAe32aa13bdmutant
mAc00d32cedmutant
mA249c796c6mutant
mA9a02c7569mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAe32aa13bd mutant 1 46A e32aa13bd_slices_1-2-x-3.zip
mAe32aa13bd mutant 2 61A e32aa13bd_slices_1-2-x-3.zip
mAe32aa13bd mutant 3 41B e32aa13bd_slices_1-2-x-3.zip
mAc00d32ced mutant 1 12A c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 2 53A c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 3 45B c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 4 52B c00d32ced_slices_1-2-3-4.zip
mA249c796c6 mutant 1 46A 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 2 53A 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 3 46B 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 4 62B 249c796c6_slices_1-2-3-4.zip
mAc04cc9146 mutant 1 46A c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 2 42A c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 3 58B c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 4 42B c04cc9146_slices_1-2-3-4.zip
mA9a02c7569 mutant 1 46A 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 2 43A 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 3 56B 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 4 52B 9a02c7569_slices_1-2-3-4.zip
wA41f70cdb5 WT 1 58A 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 2 52A 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 3 55B 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 4 42B 41f70cdb5_slices_1-2-3-4.zip
wA5745bea3b WT 1 37A 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 2 61A 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 3 24B 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 4 42B 5745bea3b_slices_1-2-3-4.zip
wA353fea3aa WT 1 38A 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 2 52A 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 3 46B 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 4 52B 353fea3aa_slices_1-2-3-4.zip
wAdcbbf1abf WT 1 87A dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 2 52A dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 3 46B dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 4 52B dcbbf1abf_slices_1-2-3-4.zip
wA766917d1a WT 1 87A 766917d1a_slices_1-2-x-x.zip
wA766917d1a WT 2 54A 766917d1a_slices_1-2-x-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAe32aa13bd mutant 1 46A e32aa13bd_slices_1-2-x-3.zip
mAe32aa13bd mutant 2 61A e32aa13bd_slices_1-2-x-3.zip
mAe32aa13bd mutant 3 41B e32aa13bd_slices_1-2-x-3.zip
mAc00d32ced mutant 1 12A c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 2 53A c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 3 45B c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 4 52B c00d32ced_slices_1-2-3-4.zip
mA249c796c6 mutant 1 46A 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 2 53A 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 3 46B 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 4 62B 249c796c6_slices_1-2-3-4.zip
mAc04cc9146 mutant 1 46A c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 2 42A c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 3 58B c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 4 42B c04cc9146_slices_1-2-3-4.zip
mA9a02c7569 mutant 1 46A 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 2 43A 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 3 56B 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 4 52B 9a02c7569_slices_1-2-3-4.zip
wA41f70cdb5 WT 1 58A 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 2 52A 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 3 55B 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 4 42B 41f70cdb5_slices_1-2-3-4.zip
wA5745bea3b WT 1 37A 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 2 61A 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 3 24B 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 4 42B 5745bea3b_slices_1-2-3-4.zip
wA353fea3aa WT 1 38A 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 2 52A 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 3 46B 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 4 52B 353fea3aa_slices_1-2-3-4.zip
wAdcbbf1abf WT 1 87A dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 2 52A dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 3 46B dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 4 52B dcbbf1abf_slices_1-2-3-4.zip
wA766917d1a WT 1 87A 766917d1a_slices_1-2-x-x.zip
wA766917d1a WT 2 54A 766917d1a_slices_1-2-x-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAe32aa13bd mutant 1 46A e32aa13bd_slices_1-2-x-3.zip
mAe32aa13bd mutant 2 61A e32aa13bd_slices_1-2-x-3.zip
mAe32aa13bd mutant 3 41B e32aa13bd_slices_1-2-x-3.zip
mAc00d32ced mutant 1 12A c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 2 53A c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 3 45B c00d32ced_slices_1-2-3-4.zip
mAc00d32ced mutant 4 52B c00d32ced_slices_1-2-3-4.zip
mA249c796c6 mutant 1 46A 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 2 53A 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 3 46B 249c796c6_slices_1-2-3-4.zip
mA249c796c6 mutant 4 62B 249c796c6_slices_1-2-3-4.zip
mAc04cc9146 mutant 1 46A c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 2 42A c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 3 58B c04cc9146_slices_1-2-3-4.zip
mAc04cc9146 mutant 4 42B c04cc9146_slices_1-2-3-4.zip
mA9a02c7569 mutant 1 46A 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 2 43A 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 3 56B 9a02c7569_slices_1-2-3-4.zip
mA9a02c7569 mutant 4 52B 9a02c7569_slices_1-2-3-4.zip
wA41f70cdb5 WT 1 58A 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 2 52A 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 3 55B 41f70cdb5_slices_1-2-3-4.zip
wA41f70cdb5 WT 4 42B 41f70cdb5_slices_1-2-3-4.zip
wA5745bea3b WT 1 37A 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 2 61A 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 3 24B 5745bea3b_slices_1-2-3-4.zip
wA5745bea3b WT 4 42B 5745bea3b_slices_1-2-3-4.zip
wA353fea3aa WT 1 38A 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 2 52A 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 3 46B 353fea3aa_slices_1-2-3-4.zip
wA353fea3aa WT 4 52B 353fea3aa_slices_1-2-3-4.zip
wAdcbbf1abf WT 1 87A dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 2 52A dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 3 46B dcbbf1abf_slices_1-2-3-4.zip
wAdcbbf1abf WT 4 52B dcbbf1abf_slices_1-2-3-4.zip
wA766917d1a WT 1 87A 766917d1a_slices_1-2-x-x.zip
wA766917d1a WT 2 54A 766917d1a_slices_1-2-x-x.zip

Data Files

File NameSizeChecksum (SHA256)
9a02c7569_slices_1-2-3-4.zip4566631609d9bdf8bd073a5407507db44f2bd72a422100d02d68aefe96eed4cc6eb07102
249c796c6_slices_1-2-3-4.zip6125691864746b4ed926bf293808e996eca00925d2502b9935820a9b3d603d51f11784d5
5745bea3b_slices_1-2-3-4.zip49369133b5fdb7adbbf4e6e94afd78492f3fac840fd3981126ce854aceedcb2ab17ee984
41f70cdb5_slices_1-2-3-4.zip307559406fb5f81cb6a2e79ce4d27a778f6f7bab4cf6906011e9a6fe573af1ef1ef6d2ca
c04cc9146_slices_1-2-3-4.zip316542068c6d5b9abff51cbed38b23be5c5bd3458d48178c7ffcc75e1c17d6762403b193
353fea3aa_slices_1-2-3-4.zip331788297a05dce11a425a8c9dfc3eada838460c15c1357d2c7ed05dda52f89c14af2abb
c00d32ced_slices_1-2-3-4.zip3009798413119bd8cbac9499fe68e90d866b8e55e1fa326219907d6f5677c674b6a418f6
766917d1a_slices_1-2-x-x.zip31023547924509b35efbb8e9a0ed90647f0f75dd3b2628b08141b3f4024d163e447da45d
e32aa13bd_slices_1-2-x-3.zip30078676cdf454a25d4be0480ecf197e5f2d052c879a88995823fbb9cbbc0fe518ef5c61
dcbbf1abf_slices_1-2-3-4.zip349205742302e946f05848db0a591c1aca83bb956eda12b3a5f94ce47ca017bc0c1ae79b
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