G2C::Behaviour

14-3-3 θ knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 16 behaviour variables

Mutant mice showed little overall behavioural difference from wildtypes, with four of 16 behaviour variables significantly impacted in these mutants. In the elevated plus maze task, two behavioural variables of five were significantly impacted in mutants. In the open field/novel object exploration task, one behavioural variable, NOE vs OF distance travelled, was significantly decreased in mutants. In the fear learning task, one behavioural variable, fear learning trial effect, was significantly decreased in mutants. Rotarod, contextual memory and cued memory tasks were unaffected. Definitions of variables may be found below.

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The G2CMine data warehouse provides cohort summaries and individual mouse observations from the 14-3-3 θ knock-out line phenotyping.

 

Variables shown are: EPM total distance, Total distance (cm) travelled in any arm or central zone of the EPM. EPM max speed, Maximum speed (cm/s) travelled in any arm or central zone of the EPM. EPM % time in open, Percentage of time in the open or closed arms of the EPM spent in open arms. EPM time in centre, Total time (s) spent in the central zone of the EPM. EPM max speed, open vs closed, Difference between the maximum speed (cm/s) observed in the open arms and the closed arms of the EPM. OF, NOE total distance, Total distance travelled (log₁₀ cm) during initial exposure to the open field and in presence of the novel object. NOE vs OF distance travelled, Difference in distance travelled (cm) in presence of the novel object and during initial exposure to open field. RR naive fall time, Fall time on accelerating rotarod (log₁₀ s), naive performance in session 1. RR learning, Learning on rotarod, measured as increase in fall time per trial (s/trial) in session 1. RR memory, Memory on rotarod, measured as excess fall time at middle of session 2 relative to middle of session 1. Fear learning, trial effect, Fear learning, measured as extra % time freezing before third trial compared to % time freezing before first trial. Fear learning, tone effect, Fear learning, measured as increase in % time freezing due to third tone compared to increase in % time freezing due to first tone. Contextual memory, mean, Contextual memory, measured as difference in % time freezing during first 120 s re-exposure to the box compared to first 120 s in the box on previous day. Contextual memory, change, Contextual memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the box. Cued memory, mean, Cued memory, measured as increase in % time spent freezing during 120 s of tone re-exposure compared to increase in % time spent freezing during initial tone on previous day. Cued memory, change, Cued memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the tone.

 

Variable Units Wildtype M (n=13) Wildtype F (n=14) Mutant M (n=12) Mutant F (n=11) P(sex×mutation) P(mutation)
EPM total distance cm 719 (86) 792 (40) 1010 (71) 1016 (71) 0.62 0.00047 ***
EPM max speed cm/s 15.4 (1.3) 17.4 (0.8) 20.5 (1.7) 20.6 (1.6) 0.47 0.0036 **
EPM % time in open % 28.6 (8.6) 12 (3.9) 15.8 (4.2) 18.1 (4.9) 0.11 0.56
EPM time in centre s 122 (17) 116 (16) 122 (14) 139 (18) 0.49 0.47
EPM max speed, open vs closed cm/s -3.8 (1.5) -8.6 (1.7) -8.7 (1.9) -7.5 (2) 0.098 0.29
OF, NOE total distance log10 cm 2.92 (0.05) 3.22 (0.09) 3.13 (0.1) 3.14 (0.08) 0.082 0.4
NOE vs OF distance travelled cm -59 (61) -359 (138) -570 (151) -395 (130) 0.064 0.033 *
RR naive fall time log10 s 1.04 (0.08) 1.21 (0.1) 1.18 (0.12) 1.33 (0.12) 0.95 0.23
RR learning s/trial 1.8 (0.6) 1.6 (1.3) 0.5 (0.4) 3 (2) 0.28 0.96
RR memory s 12.4 (4.3) 5.4 (5.3) 6.7 (3.9) 6.9 (7.1) 0.5 0.69
Fear learning, trial effect % freezing 63.9 (6.1) 60.3 (7.3) 41.1 (7.6) 44.3 (9) 0.65 0.013 *
Fear learning, tone effect % freezing 8.5 (6.5) 11.8 (5.8) 7.6 (7.6) 4.6 (6.6) 0.64 0.55
Contextual memory, mean % freezing 48 (6.6) 48.1 (5) 43.1 (6.7) 29.3 (4.5) 0.24 0.051
Contextual memory, change % freezing 22.9 (7.5) 34.6 (7.6) 23.4 (8.2) 26.6 (7) 0.58 0.63
Cued memory, mean % freezing 0.2 (6.4) 7.8 (5.6) -1.4 (6) 0.1 (7.7) 0.64 0.47
Cued memory, change % freezing -6 (5.1) 9.2 (6) 1.4 (8.3) 4.4 (8.4) 0.38 0.85

 

Elevated Plus Maze Performance

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Variables shown are: EPM total distance, Total distance (cm) travelled in any arm or central zone of the EPM. EPM max speed, Maximum speed (cm/s) travelled in any arm or central zone of the EPM. EPM % time in open, Percentage of time in the open or closed arms of the EPM spent in open arms. EPM time in centre, Total time (s) spent in the central zone of the EPM. EPM max speed, open vs closed, Difference between the maximum speed (cm/s) observed in the open arms and the closed arms of the EPM.

 

Variable Units Wildtype M (n=13) Wildtype F (n=14) Mutant M (n=12) Mutant F (n=11) P(sex×mutation) P(mutation)
EPM total distance cm 719 (86) 792 (40) 1010 (71) 1016 (71) 0.62 0.00047 ***
EPM max speed cm/s 15.4 (1.3) 17.4 (0.8) 20.5 (1.7) 20.6 (1.6) 0.47 0.0036 **
EPM % time in open % 28.6 (8.6) 12 (3.9) 15.8 (4.2) 18.1 (4.9) 0.11 0.56
EPM time in centre s 122 (17) 116 (16) 122 (14) 139 (18) 0.49 0.47
EPM max speed, open vs closed cm/s -3.8 (1.5) -8.6 (1.7) -8.7 (1.9) -7.5 (2) 0.098 0.29

 

Open Field and Novel Object Exploration Performance

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Variables shown are: OF, NOE total distance, Total distance travelled (log₁₀ cm) during initial exposure to the open field and in presence of the novel object. NOE vs OF distance travelled, Difference in distance travelled (cm) in presence of the novel object and during initial exposure to open field.

 

Variable Units Wildtype M (n=13) Wildtype F (n=14) Mutant M (n=12) Mutant F (n=11) P(sex×mutation) P(mutation)
OF, NOE total distance log10 cm 2.92 (0.05) 3.22 (0.09) 3.13 (0.1) 3.14 (0.08) 0.082 0.4
NOE vs OF distance travelled cm -59 (61) -359 (138) -570 (151) -395 (130) 0.064 0.033 *

 

Rotarod Performance

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Variables shown are: RR naive fall time, Fall time on accelerating rotarod (log₁₀ s), naive performance in session 1. RR learning, Learning on rotarod, measured as increase in fall time per trial (s/trial) in session 1. RR memory, Memory on rotarod, measured as excess fall time at middle of session 2 relative to middle of session 1.

 

Variable Units Wildtype M (n=13) Wildtype F (n=14) Mutant M (n=12) Mutant F (n=11) P(sex×mutation) P(mutation)
RR naive fall time log10 s 1.04 (0.08) 1.21 (0.1) 1.18 (0.12) 1.33 (0.12) 0.95 0.23
RR learning s/trial 1.8 (0.6) 1.6 (1.3) 0.5 (0.4) 3 (2) 0.28 0.96
RR memory s 12.4 (4.3) 5.4 (5.3) 6.7 (3.9) 6.9 (7.1) 0.5 0.69

 

Fear Conditioning Performance

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Variables shown are: Fear learning, trial effect, Fear learning, measured as extra % time freezing before third trial compared to % time freezing before first trial. Fear learning, tone effect, Fear learning, measured as increase in % time freezing due to third tone compared to increase in % time freezing due to first tone. Contextual memory, mean, Contextual memory, measured as difference in % time freezing during first 120 s re-exposure to the box compared to first 120 s in the box on previous day. Contextual memory, change, Contextual memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the box. Cued memory, mean, Cued memory, measured as increase in % time spent freezing during 120 s of tone re-exposure compared to increase in % time spent freezing during initial tone on previous day. Cued memory, change, Cued memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the tone.

 

Variable Units Wildtype M (n=13) Wildtype F (n=14) Mutant M (n=12) Mutant F (n=11) P(sex×mutation) P(mutation)
Fear learning, trial effect % freezing 63.9 (6.1) 60.3 (7.3) 41.1 (7.6) 44.3 (9) 0.65 0.013 *
Fear learning, tone effect % freezing 8.5 (6.5) 11.8 (5.8) 7.6 (7.6) 4.6 (6.6) 0.64 0.55
Contextual memory, mean % freezing 48 (6.6) 48.1 (5) 43.1 (6.7) 29.3 (4.5) 0.24 0.051
Contextual memory, change % freezing 22.9 (7.5) 34.6 (7.6) 23.4 (8.2) 26.6 (7) 0.58 0.63
Cued memory, mean % freezing 0.2 (6.4) 7.8 (5.6) -1.4 (6) 0.1 (7.7) 0.64 0.47
Cued memory, change % freezing -6 (5.1) 9.2 (6) 1.4 (8.3) 4.4 (8.4) 0.38 0.85

Acquired (raw) data

Animals tested in the G2C behavioural phenotyping battery

Cohorts are summarised by genotype and sex, followed by a list of all the animals from the line tested. Shown are the unique radio frequency tag id (RFID), Genotype, Sex, mouse batch identifier (Batch ID), date of birth (D.O.B.) and data of RFID chipping (D.O.C.) for each mouse.

View experimental protocols.

 

Behaviour Cohort Summary

GenotypeMaleFemale
+/+1314
-/-1211

 

Individual Mice

RFIDGenotypeSexBatch IDD.O.B.D.O.C.
015872270+/+FBATCH_08-4215/10/200827/11/2008
015886544+/+FBATCH_08-4225/09/200827/11/2008
015889106+/+FBATCH_08-4215/10/200827/11/2008
016017532+/+FBATCH_08-4225/09/200827/11/2008
015883540+/+FBATCH_09-0112/11/200807/01/2009
016573042+/+FBATCH_09-0112/11/200807/01/2009
016540373+/+FBATCH_09-0220/11/200808/01/2009
014268293+/+FBATCH_09-0508/01/200905/02/2009
016554846+/+FBATCH_09-0521/09/200805/02/2009
011024821+/+FBATCH_09-0804/02/200912/03/2009
011033890+/+FBATCH_09-0804/02/200912/03/2009
011096378+/+FBATCH_09-0804/02/200912/03/2009
011031117+/+FBATCH_09-1128/02/200923/04/2009
011066336+/+FBATCH_09-1430/03/200909/06/2009
015869098+/+MBATCH_08-4218/10/200827/11/2008
015881576+/+MBATCH_08-4221/10/200827/11/2008
016003559+/+MBATCH_08-4218/10/200827/11/2008
016012060+/+MBATCH_08-4223/09/200827/11/2008
016538562+/+MBATCH_08-4226/09/200827/11/2008
016577005+/+MBATCH_08-4218/10/200827/11/2008
015870356+/+MBATCH_09-0104/12/200807/01/2009
015881021+/+MBATCH_09-0120/11/200807/01/2009
015895601+/+MBATCH_09-0104/12/200807/01/2009
016038121+/+MBATCH_09-0113/11/200807/01/2009
016583281+/+MBATCH_09-0120/11/200807/01/2009
015894104+/+MBATCH_09-0527/12/200805/02/2009
016015836+/+MBATCH_09-0527/12/200805/02/2009
015880062-/-FBATCH_08-4223/09/200827/11/2008
015894059-/-FBATCH_08-4215/10/200827/11/2008
016557835-/-FBATCH_08-4225/09/200827/11/2008
016578089-/-FBATCH_08-4226/09/200827/11/2008
016566322-/-FBATCH_09-0220/11/200808/01/2009
015872338-/-FBATCH_09-0508/01/200905/02/2009
016025614-/-FBATCH_09-0509/12/200805/02/2009
011331836-/-FBATCH_09-0804/02/200912/03/2009
011348857-/-FBATCH_09-0804/02/200912/03/2009
011356286-/-FBATCH_09-1114/02/200923/04/2009
011368369-/-FBATCH_09-1431/03/200909/06/2009
015869842-/-MBATCH_08-4215/10/200827/11/2008
015881793-/-MBATCH_08-4225/09/200827/11/2008
016005582-/-MBATCH_08-4225/09/200827/11/2008
016022072-/-MBATCH_08-4223/09/200827/11/2008
016035332-/-MBATCH_08-4221/10/200827/11/2008
016553519-/-MBATCH_08-4215/10/200827/11/2008
015892615-/-MBATCH_09-0112/11/200807/01/2009
016542606-/-MBATCH_09-0101/12/200807/01/2009
016559123-/-MBATCH_09-0104/12/200807/01/2009
016583056-/-MBATCH_09-0113/11/200807/01/2009
016535286-/-MBATCH_09-0212/11/200808/01/2009
011098378-/-MBATCH_09-0527/12/200805/02/2009

Elevated Plus Maze

File NameSizeChecksum (SHA256)
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015881793.mpg104857605e305428a2fa980aaa33a4545f25d6bf6e5ba22b904c21cabf0cbb008de8b55f
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011098378.mpg10082304c28e02e6f61ee1d9e20410cbecfaeb3cc8e2dfe1d9b5916065920f9e5ee45104

Open Field

File NameSizeChecksum (SHA256)
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011354776.mpg1334067203da6ce14e6b3166dcd2dd3c99cd5df82c1f9dbff3849c2f6f5520cde4db56ff
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015889106.mpg82944004250bc307d4717162a563246ce6e25aa98587c3b7c67b9a9fb2375baf39598b1
011368369.mpg143114242c213d3b830ef6d96245665d8a290310656e8d42aeabbb8c936392cf01854c66
016035332.mpg8790016123028ea0fb8c6a86e78c2d342449e9e275f0c3c0133f257673a131a62b07533
016554846.mpg1322393602b0f8e6356cc43c02c27ae3233d0c438d3c82f787c6ff10895dc8af5327f368
016542606.mpg85073925ecdd6521187613f399554e8f508ad75e9b90e2de0213f904f1938762e2b66e6
016566322.mpg8845312e29d605aae6fef601fdd181c1cb59f69143354c4e1ca09da83e7d72e0ccde8a7
016015836.mpg138076167029dd1d6f97137d6a00375d1999bc7dd0ca691f7951d2928037a75ea6dfe481
011316608.mpg13512704105d2ea0afdf3b4fefc26c3b0e2a2cafded85b2fe7913df2e4923dbd2a840f5a
016038121.mpg12947456dcd32e9cdb09fb3cb5113c897a78961f2b5f6d26a55b094b1a7fcb616a600420
011031117.mpg10782720372d49fb77804c2a58984d3958f68d045a4d30dd6e6b6e1da339b360e046d31d
016583056.mpg126545929a78aa3a31cf529c6191c56f74182fd5073341edfa400af525a46d4b44bef322
015883540.mpg921395288dee25ce68897226468eb14d5354837d7d48769d6efe0ade134dd9e6824b721
015880062.mpg8259584c2097db2d741c57172f76d378ac847121b41fd38f6501ff39850de1e01098df1
016012060.mpg92323842670b5d3bae5697cf1518b8bacc90dc9f36ed9ff862907a5b0001c0eb5438f87
016535836.mpg10858496ed6526c74e50dcbc85b94e7649b5061604cdea5fb8246baf704d5b8dbae4b5a0
015881576.mpg10344448950479ba7f9d3cb1269605ea47ab5c450e630344895ac548e766a621e7961e44
011066336.mpg12392448c63a34589599ae14b2c99cc51f6eb5fc20e6f587aafa576cec43b6f34f08fb56
015881793.mpg10637312899c21e4baa965bc2a8b2fa194cbc625c70b5c11aa0bc1e9cf8e0a36bc3fc633
015869842.mpg8364032c4269de67188646dc9b876a8552fa947cdf733afd744df4a7539a87730f1ecc7
011331836.mpg853811206be686b3432afb2b06cbab011524d05b619d0879cefebcec1b1ebc987aba63b
016559123.mpg1070284877484041b99ef0275d1fd00a72dd75ade66728ba701f5a9100707f633d0e343a
015872338.mpg130990084cc2aefaf7670ebb5489ddd0672f47ea8f8eae45df4e7a2fc0e0954574df83e0
015895601.mpg10475520602c5bfa7c3a21292d84829fb3e455afc3c9245b1b68465d84a13b6bbd10570a
016557835.mpg8378368b4c5c475ed41366c09b4580d62d00336c682f0ed113e35e12c3acba704c9b76c
015894104.mpg1299456045eee348d7e06a31e84ddaf194808e8765f79ed794573dd475198e22da48e22b
015881021.mpg1330995265c6e0d42b2ea9b05ee1d5a01b5855a16210a21f839c223b65ac1751226656c8
016577349.mpg13068288608fab18cff9b2b0e52012a17089b83988242efbcaba73cd86e1b62ae74308f5
016540373.mpg1094041639847fef3a22de479c1872843185bfe177ae8c4a612593ef8f34f1cdddb61f54
015872270.mpg10258432980b08c429807d455bce125d9e053077ed9ba0937107a221c48b8c86db9e2a4c
015870356.mpg8736768be4244451e3c8fa65aeb35db2dd6e7961772342ea33e450cfe5fab2719ecfb5e
016579601.mpg1301299241886f15cc8b41ff4b42114034e2faf10046b166a31fd4a410988fe73d9d2557
016022072.mpg10899456a8eb074aacd4fdb641e67943f92807033677aceaea52dbac5df9709a2c695063
016025614.mpg129884168fa65a08cea0bde1d7f71933de53313dfa5d9be9d1cb56a6fd0c5ff3ca8f5838
016535286.mpg12765184e9511e013cf01262b93c809e3c06eb716f4ab4c5a3ba1e93ea2337f8f58cc1b7
011033890.mpg847052879e4ff9c20fc84eb3ed3b6dbc755cdfaa6c075aecdca15e8d95720254e2dd78b
011098378.mpg1335910403d22f01fc82b0d9fa224259fbb21315cec909e7cce1cb123eecf40ad37a155e
011348857.mpg105369606982d4d8e79a3834fb792a1542fe23d7813cf0f110356abb701f54b5036edbf5
015892615.mpg8976384550efb33fcc14f92d15519f033ef7413507bd61114d0c4fc806598355ba16885
016017532.mpg9947136649859bb5640979fb87b1af98ccbffdc936e033d00bac987fe5aadb39ea91474
015870638.mpg868352045a57e67e17c80b536f8bf4db4429dd43e4dee18aee832d4807f51199f42cb4f
016580373.mpg103936005d29b412c31dbc119c335a097ebf1921e1c854fdfc21352043693da60bc99734
015886544.mpg123269121d1a2e218b4fa3459922efb56e8f1ccb34d7c878906d6e1c2b9df1454291661f
015886775.mpg10475520c35a442793116eb46f30b6d71d926db96ca3fe861b623021fbe821dd0713ed7a
011024821.mpg8574976363dab2b12cf6c51f11dd7c2ef46f646712f8e977def7c33953b261b349007f9
015894059.mpg828620804933cd69f72ca290bea01a5041ab33dc11db0c794ae45a5ee3f293f66f11533
016003559.mpg8581120ee41d0bcec9c6ce747816a27fb07cb931ada4249b8d4175b57b0d6e93212a1ac
014269277.mpg1067008019ed1c9e7b10f8d688358cbd3f731a7280b96e62c2198759f4a4e2bdfa4b8298
015869098.mpg10579968d3a65bf1bce78c677a20772588c4c41881173201afd9ebe51b8ffeb0dfcba6e6
016578089.mpg828620811e6d606892ef5704f83787e68c4d2e8b01caf04b5b3834a184611fc55445fc3
016553519.mpg10231808532c2fe851b3bfdcf305ba5d61be458639517e3c71d01ae009944d15536a60a1

Novel Object Exploration

File NameSizeChecksum (SHA256)
014268293.mpg130273287b33d76f689e93bef73fd1674ec37286a7f5c2ef06cd7d01b506d04b594b7cde
016005582.mpg84254723da0e491e0d14769099017883a4076e710fd0436bbf8d86c8d7e100355c91765
016003559.mpg8757248033758a82880b0b95edaea36cf60e796599a4fe3ef1fb952d4c594f51db48f75
015870356.mpg88166408baf982c597de0ad9495998089a37869fbe04f949ca2d320f6d54316556bb1cb
016559123.mpg109199363343a44625e290385356fbb72139e1297699a97c9ab1dcc01f1c24d4cd1f0d08
011348857.mpg10510336fffd905648f07a2e10128ce9f8ed2ac1fb4460fdabe188067c52333a01fcfb8a
016017532.mpg83476483b9d2995413b1f23742b31d6ebcc6e3d6fc447e89c18b93e41a2f04b42d1a4e0
016004064.mpg10555392ed5cd779c0a43bdaa50492d71425b252359ff6a02e2af18009fb55e4f0def9c4
011331836.mpg8472576220c49a57634fc10b320faa5fad767065afc9fb9bbf3684f1fb913f2d293a618
011098378.mpg13518848e721cd371cf3adbe9b5692b49440d09685641ba924700cb828074dfafebee51b
016577005.mpg11317248d7a4db2d2c1a529590a4c3880bd41a555a6c6b4db0549a6f578e27e114d1b45e
016535286.mpg86876166972bdcd67d1b25a04fa94cabf89566ceaaed7661cb8dddc112a1e70eb645a15
011316608.mpg1298432085a69d345fb4dd477097c7912d5a437c0c1f028092f866c6e03da0d82dd5790b
016583281.mpg10553344a0d02f4b48952fc59a0cca4a616a051ace43623e362781a318ba05b96748f129
016038121.mpg1043865635eeac5b1753613c94b194a08ba2b7ab5559e62613f5be7556e927c7bcc9a08a
015894104.mpg132075521465b1aab6f2315989978292282341c3ea38eea6a79149f8f878c90f008a651d
016580373.mpg877977687d29409f7bf7e0370f835c6508a8490423308a4ce798b0bea0f7e184882ac4e
016540373.mpg11151360ddebd405fb15370c9807305a17948ca1fc748d3ccada769c56fdf5d0000d2cc2
015881576.mpg113336322d564570cb72c4f5c2f569e530a01cba678ba81d0529bff09da00bec64e8d672
011024821.mpg8726528cfa2079c4f56a855866c7bb35bc2b8eefefca7e0ca7df87089f6ac88b9fa4587
011354776.mpg136540161a8a5d17132ff009997872c69b6464d9866ddf563d163607fe1589a328c35bba
015894059.mpg8626176f77b724e4ebb806eb7b25c2ae295f35605f158e6771ccf139d145caaf16dfcc4
014269277.mpg10823680270df257526f330be18a86df929a6f9754e311c001bc21908a6db1e72f7647fa
015872270.mpg107929604cb8091247e32d7b1dd31016dda89ee70dee6cd43fa507b571233f99f02f4468
015869842.mpg9138176e72b0124ff016571ee140f1f8b9d33d048a4b626032b16fd55bac48a3523805a
016035332.mpg903168063505572b6865ecda218679776925fa119182b5985104cfc50efc084716f0678
011368369.mpg13674496c0359445aba062a11c49358b9cb9242d2999f832cb0a954461b78d29801a823f
016578089.mpg8654848ef93a326608f5be96b5b44f49eb9130529949f061332ba9a7d752b1109ae23d4
015889106.mpg109383682a5de974066b124dd73feed9716e9d3a88f31c714ff40323f7e47d389581e30f
015895601.mpg10461184f1037cf11dfb56d000f3f219444ff02b9b435fef754bc4aa82e4dde5042b1061
015881793.mpg104734722f01275ee774c9b2e51e007851fd55f3a4c3b750f36485a1d43bb138a7b435c1
011066336.mpg1293312002f4c40eec1383625335ed915941de85d226ef9c66e33d060fbc0774b31ca2b7
016542606.mpg9175040f765316866b326e6a6c239c802d5d10429bf534d2c4976825fbe4b92d512183f
016579601.mpg13223936db4d8a72c37971b0e513862de35a58f49a76fdb8c8de24a1958c8ab7ae71090f
016553519.mpg10735616ec24c561d38620c1fe1cbcd1a9fcd3b02bdb8d4323a23a465557a3da93305541
016538562.mpg10653696e051be16c9a6bed266dff902b6ff20f02d2cf8259710a152dc371f410f3140ed
016012060.mpg89169925a560f46f1e4918e04826dabb7e3d4eb01ee30e8a4c71e87704603e2a987bcd3
016554846.mpg1366630433075921813f8c4145d327a356da4cafd51b3318261aad4b0bbac2d9aca3acc3
015886775.mpg11204608efe41cfef042a2cf7fa19f76f5f67b0a88c7ec78272b1cf255e6032857633bc9
016015836.mpg135208960e995d72497765925d14621aba799bab829566e257904eee78dacbe58c447378
016573042.mpg15196160602bac61128a85d114baa74758b0b74cda0e5d89c9641b36b95d5b19f021ebfc
016535836.mpg1054310473eca4f55a21bc7666b1bab8fb32faa3b4c9159309aa7783338bc5b84cf9a731
011031117.mpg105697283e864a48a1be9da54a2acd83b7f4733cf6d982baf5aaa31d9bcf2b0a4db5a894
011033890.mpg85626885efef8abd89adad0272e773fdcc0b0bc0947fab0f36b02c80499429447e0e5fd
015886544.mpg1028915297044cb7b256bdbe5bcd0e8c4f75615280d69517acd396836a158fe87d95afad
016557835.mpg8480768873ba35878809a7935a3af5837ba4b047f2955d39584828746d7e97bccd75b06
015881021.mpg8769536248cdeecb67bc73154d1eaf791237277c744701ee00cbf78f736794ead762907
015883540.mpg123514889fdb9f61bdde375847435d189f96c823b344f66d8628c46860de9626f69ce1fc
015872338.mpg130150407c370f8f21eba287a50de132391513519f5ff99ddea83e9a944c0c67c6c740a2
015892615.mpg857292881f2cb2f313663a9700e5f4857f481f489503b3b885e0190f8e33be12bf8310d
016577349.mpg10833920223d26350e0aa006a74947e48a22a757dea43c71af2f3a1d7fde26ee269b390a
016566322.mpg895590422307b57763f75ee3be531dfa2d1ca00d8c144a1bebfe59266769fe7755603c0
016025614.mpg136130564e1082003ce8b986f0504bffacd32664b5c0ba48a71f0989fbe01be4da8100c8
011096378.mpg1059430469b33d39b73cb24ec6b300cfa02714cd73119e0ca62de615f240fd736aff6d9c
015880062.mpg8736768175e7ac2d7b3cce02bb6a56de1ce2ab347ec045484f238a7ecda2c50cebbd0b9
015869098.mpg10946560c894f0242f064916c5b1a4e3b6e3c24293ea3e5d0cd6fd87f5117d9c21f0978e
011356286.mpg9052160860a61669a3a45ced7088dafe9cebf26be0ab9afbba26c42f7fb3f7b99c45312
016583056.mpg87019520f456e8f20707aef73c66319fd604a8d54c7e0f2aa8295d90d525831ca00641f
016022072.mpg11114496985b9e3ce1548f9534efd1dfa95c4df3eb9a4dad2941479794fce8caaa6e6864
015870638.mpg8531968692ee40eb323eb15f41dbd40cf518ff60253a19b788e9f18d58271163c41e059

Rotarod

File NameSizeChecksum (SHA256)
016553519.txt4821dce910515b45821ba17e4823ad53c3ee185fa9e55d71215c612ce36a678070b
014268293.txt49714e52a141433ca354624d3bb7c960ce85295ddb467ffffc7836ea28dcc85ef48
011096378.txt500e70ca6e6190fb454f4221d4676433d70dc6e5072eede93c03a7ffe9874b66d77
015883540.txt4948793a78392ae76724e1dadc3bdfbfd6212739bc01e8f223c00f636b017399516
011356286.txt490aca5466bb5681a7d9a7537cbf91f90aa939db2cd77c69f77858783f2a99259fd
016015836.txt4867837d192ba1c6e5e69d136001f8b40f0ba45cabd11b991c921498e534e6ae7ec
016038121.txt482c4e9c69c9ed7c1022dabd3b821578ac01642155ad41ac54c10fdeb12ad9db51d
016022072.txt493712bc281203de8b744ad9a7fbfddf75c3a7e0543675600ae0aceb2f814a4dd90
015872270.txt4946548b7be7eb1b8545f5c850a551e650d7df83d8ba4358daa897dbdda35d0b241
015869842.txt500ace16888a00d142a789f7554d5a30a40255c54a700a71b9f0ab72e4d3cffb06f
011368369.txt491a63dd8b1d912a09ee916a0977836119d408dd81f956961ba5ce7c1466587c187
016583056.txt4822f1027ae80c4de49cfd757ddd8a13a4dfc42a22ac596d4ab9603af97e3dbcd89
015872338.txt4871ee7154b6f248bfbf9ffec27fc9058a7b2410926e8e9983009ef715d5811a102
016538562.txt4815bd03df0fcabb6273c7b91dc7812831933a0055c2af24b2fa6c8634cea4d0852
016025614.txt49558f8403c01e4b1c167e06e260ac1c1d1a903fbca445ec9d504304cab081c27ca
016005582.txt481200f76148e6c97bd0928c7e4aa70413f8386d61fe5532c449967a6bfa656b179
011066336.txt4846fa7ce5aedd32989ec0a2394582b76b35b0547fa37c948ebcacd5e3b4b059208
011024821.txt482b9f044d3ee0c94a8ba6fab7ae30dd2b119ea9cfd34fd3fc9cf4420a01b393b94
016583281.txt491d3678d84603616898d7945968c4f13f3d284dd50fe4e68a97f77318cce76afff
015886544.txt49149f976a2090f358dcb4971876939beee4ad4cdc1749783d12760a371c0586821
016017532.txt4882319674c01e601cef4f7ec0aa02edaa08932fcbd155c155c94bde7cb8c43c039
015881576.txt493ac2faa523abb425dbf297ae15062360970ae9791fc10d866942c835fb4c21bd5
016003559.txt496e10919552f99dfa1ba1b1850ba89c9acf9d03ca66dcc4b8ecc4aceffa583385f
011033890.txt47925bf0b2266aa29c3a3a0bc4ce8d4d8600d4ed2bace49434b36e3a035ca8999e5
016578089.txt505dd469a4fdde564d85bdde2740b06a642e72118f88e88003e2893702277123361
016559123.txt486645c5cfe5da194514a0fc1507632d16cdc3f3dbe12410281f5ea43a42423bffd
016577005.txt482389b7cfc8d1f8fb527f72cffcfa5b8501fab12366623a0dd14d3b163ea16732f
011031117.txt490d762d7509fe5c9502aa93637ef936f12eeb9931fcb97ff67165b43a02de89b10
016035332.txt493aa2c9a02af326bd7c9135284af785da910c69fd73a9b8ad7c270557b61252df6
015894104.txt4893b878506b0e31807986031c1b1e46e5b8bdd19fe568cdad4ad79b96ebc01a353
011348857.txt4867d9dd69c072b2dd03d85344055ad0ee8533bb9a000e5ba9f23fcce1442482d57
016573042.txt499b72e8ca413b99ff2187a274339ad3d6a2499f1ceb08734a6eb665d4c619ef877
016542606.txt487acbc684757bce4012d3059c6f7c9c2de1d79a130e3dcc4cf8eb2e43885d40656
016540373.txt48775b306ddcf516ff259431ddae8544086af30242e45ff9b109d515e17712452e3
015895601.txt496d0d86caea422880447811bf1da26f63bef170319425d2fd4e06a06fd6bc091c3
016566322.txt493ac58e7e9309f67ee6f4428d21df46e90fc0f3b70395b3da0f264dbf01c3f633b
016012060.txt492f53f7ce491da11ff54b1f4242a47fc33fb903003c94fb33a8d70d0437a61c5d0
015869098.txt481575376c26c040f846e423681ae21e4e7fea54493e2c47bdc8f0eee00c93f2118
011331836.txt4954ad7826246a16ef975fe58e391b4ecf18f597a5b90742497ef576eaab47dc5f0
015880062.txt488f86938275a487231e2c23dcb33cef1f6fb53e84123feb53ef7bd2cc15cf2a5f5
015881793.txt50077044ad27e79eca125f1ed0695b4508435f9154168d0df55fdc9a2f11f520da2
016535286.txt493b4ea6f92cc995a3758afc123b1a6cec4cbf401d22711d7fd54394fb4a36f9980
016557835.txt5032be044a85b5c93d922be2259ca7ee2b92f4b10c9d3644d88ab5cd0aee69802a5
011098378.txt4814574d202c51b971e398696f61843e27eb6c470e031c735765b79f004d9e5e6ca
015892615.txt491e30faa83dccd28276a0c9b01e121e539466a22c41a2832455e0573aab6dd96f1
016554846.txt4771267cf4fac607e09c8ce9be096db5d94f19980428b836d7864d7e65a7ada7f0a
015881021.txt494fce733ca784350fb0b7ae0deb0bad6295a7d804957e4d3b62ba38e5b17b4e7d1
015870356.txt493c316e77ff9ef573510ea3e8d91879d240e5d0913da5a0a9d7b397beb755f4dca
015894059.txt4925a713c87dd173dd789d9ab94a311d70842d96447cd672b5dcc7d706a722e4af4
015889106.txt494ce17facc12846f5998c017ab971d16af763674c78e9ce5ec30b7ad1c94e94cc3

Fear Conditioning

File NameSizeChecksum (SHA256)
016535286.ffii113894640e194c68fb29026f68cbb6ff5b92af9ec4b998f3bcb4c120851b686892f84a2c8
015870356.ffii113894640b3944d5bf960216c2e61d55dfc87c87889599a91108dbacabfc73a39d2e4b774
014268293.ffii11389464052d85587cb8a0154d08e0681517b0a5caded51e57025bf3bdd28254587d13db3
015894059.ffii1138946402ef98a2d0719389d79b200d6ce77a0e09660e69b597594b7c2db724562c5ed21
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016573042.ffii113894640011b3e87aaf43963e95373f4b90e46521b145ff8e4ceb40f4b8cba27f61e1281
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015881793.ffii11389464004219f55b15e0bcd998e81b8241a16b7d4367b5df012d4ac58293dda699735b6
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016566322.ffii11389464057a772169193f1bf6093176d9a39d7c0de53571b08f43fa5e8991a32693c066a
011356286.ffii113894640a59c2e25c227a8c2cfb4f90bfeebc7b7a8d89c44ae7111b1b2e7d0e2d13a5796
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016035332.ffii11389464006dd6b5bac34fb81152d202c557e89eafbb8baca49630195fbafa45aa905ddf1
015869842.ffii1138946401081995c70dea9ed12b9f0e931160e0c68e94cf0f1d6fd889fc0542447119efe
011348857.ffii113894640eb2ef681169cc162880c2bcd62f38cc8460c41a647f30a1650b1c50ae57a490a
011368369.ffii113894640f73ea963c8ef4b6c949694e56f2ee37f711e4ab0fd46cc9b51d7f80e6e563061
015892615.ffii113894640349f277e5ca9778accc9d31b3306abb222fbbe322717c17ff4f7e53be0717992
016577005.ffii1138946401a3cb3820f8eb351637b16fffccd9f058bd626811cc352a35f7770c693423723
016557835.ffii1138946403b68d60cad0248ddb3422bc8ad72c44f7a2cb0f69088a4925843faa6a42297b5
016542606.ffii1138946408acfcc85dca3e80f1e3367ab0c78a2b66cae015abf2a4015005cb4b6b950a9bc
011098378.ffii113894640f24c4fd283e0dd7de256e31ff095ed8d066b42824f0caea9dabe17cd78ba4149
016022072.ffii113894640400869a5ff175217f7db7d94907a6e8f7342bd7f13093405797eac9c8a8e45f5
015872270.ffii113894640f0f2303bff0d87a6f35adc650d66ff3b4688cb8488292eaaa0631263c705fcd4
011024821.ffii113894640c1d10fc2bdde2cc746c1be5fc64f42605bc939ab9ea38eb461858e9c546ecc91
016583281.ffii1138946402947ff0d0eec68ea7ea1a806640dc93be3c11be90f735d606104609b42dbe0a2
016583056.ffii113894640de007173ca070b2f8cecd96ef67095b86fcc562ac3064e1f67ec15dc62df94fb
016540373.ffii113894640169627f3126d70f91521c6d69020ca3acd260302314ba14fceef40770edef7a5
015872338.ffii113894640c6d8a6bf201f528ad9774a9509310008299eb7a6bbe37f2e71571eeef2e1e634
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016012060.ffii11389464068e322cc604f5e4969955ae1b6713f60b357441698bd878d7b9fb3bba809fa86
011066336.ffii1138946409a13131f2f58d2b7cf2296f2621d1c91a3cee4979ccda300ff20aa2b7158ab9d
File NameSizeChecksum (SHA256)
011331836.ffii840823204310c32f92cc31ce9a0e54413d551510c10dcc2733aff7492b4a9537b4039c38
015880062.ffii84082320eb48a1554b9b30e5a3872d1c77e73531a204f04ce05d408e8ad5e75dbbcee7b7
015869842.ffii8408232058d4239987f558142dcc931949312bb9d95e08f77027f4053273f2a59813a62e
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015883540.ffii8408232047365b6f15caaa4726949bbf91db18a72c9f39143f481e70a1e41337447a5ffd
011024821.ffii8408232061000e947cf5ea0e69f4b4711a30e5f2771d5c0b543d974c8ee21d05a53805d5
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016554846.ffii84082320ec8a52b23879966b82c393d9a509f807dc251012e449463a35c61903c476790e
011368369.ffii84082320820990d2b236ab67ef169d9306fa7a572101579f117ccf99cff571e19e805d56
016583056.ffii8408232076a0596761abdfefd44da89be94e3a2b3e55d3887082e68f0786fc226e7ea5ff
016542606.ffii84082320fcb1c865db90ecbe687dbf96bdf9333190c42186212fb7f0369f97f7073c10a4
016025614.ffii84082320bbd584dc64cde0da546ae663e1a58099722fe91e128e4b6fa98c905d022bbe06
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015889106.ffii840823200444a58033a1c3c6a031baec5a3c61311692027891358dd39b0cb188e3c1ffcb
016566322.ffii840823209002aabce4a107c6123cb5ff6c0ba3388bc4ba98479040ae1f1a15035ae017b2
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015894104.ffii84082320b32648f4cbf82db50fdba6ae43f8215e466d9ef87769b45942e1d051d4a06164
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016015836.ffii84082320387d3e155712f866656492c6963b9fe1ceefc6e8714cc2889f990af00b402411
016538562.ffii8408232026566b460d58ce7130c08a215ab4289fa17c333a5dc97b592e6f62e903424615
011033890.ffii840823203e8ce9d306e438bfebf68bf4e6cf7687bbde89db290e853fed32ea380bc4396d
016012060.ffii840823205a9171b48677755244ff5c9f0a3cd6f98325b634e3452993e47c38a9c949da5d
015892615.ffii84082320bbef56787376e45b350bceb44fc3d2a2ee924e62c4acfb644e6892561f201184
014268293.ffii84082320dce549f0f6fba70675fa4b22972a09126d9a15e57dde52bfb52b00cc446b7b8a
015872338.ffii84082320f44ecc7624bfc2a4756ab0a24873efe3a5e9ff74da56c4ceaa2361616f2eda16
016022072.ffii8408232026bb92a615f342602dab07d757076efb1cc01629ed967d09269fab2aa27d032a
015881793.ffii84082320cbd8921d4242545a504fdd93e0cecc52885b65e7cd329e9f04e4a921da7d7b00
011096378.ffii84082320f604e4451ac2e4b758f80956313626df5349f5b984aa95645f65f3e78f455788
015869098.ffii84082320821b943a56c51ee05b3444ade2e6379cd02481513db7a047c52f56b5e3a7688e
011356286.ffii8408232002f186d15c00491e32d28900431d72db5259c5a97e921fa308ed36ee2632440e
015870356.ffii84082320f94b1cb507c778d46a31196fdf3541c103c41717114491af61fe8535573d286a
015895601.ffii84082320b4c2835aee3e0dfcdeaec4c1d48de88dfa1bc322bde865061ab771d70cca756c
016559123.ffii840823202b70b4061f6859aeb79cb0e0dbd8212294c958976bb81e93d8bb1be509ea3bcb
016553519.ffii840823203d4cd26b7ed532c4c5bdc66747c9916a8341960b9ecdc7f185a67746d2f0dc99
016038121.ffii84082320bc363df4b5e6b93cc1c658e128332fa98a3bc9fc2e74b7a04d901014c7bfb7f4
015881021.ffii8408232092e77a121944b5088724cf51566f4054d7f3bf78b32cc2f017614eff98a9adaa
016017532.ffii84082320a9e1743acd077f4d99eea2e03a279f1d0faaf573bc0885eb5828c0291407f501
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015872270.ffii840823209971138630841f1cf2730dee244530c1a9d029a5f2df945bed7a67fecf3f3ecd
015881576.ffii84082320d7e2e82ca8a33a10d7dd8c4436f89a8c154ad2ddba9f6a8d54c9dfaf36d54c41
015894059.ffii840823208914a62e7305eb5feb4a899116a6c92c2053e9a2a609f41d41c273a11ec28550
016005582.ffii840823202fc1f476478459a038259def695ff49b2e9acc7d56911eeedf7285c4aa38e2f1
016578089.ffii840823203a372e8a17cbf8388ebbb9d369c1ce0bf9b2a470b09a994123988e59461731d8
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