Automated design of genomic Southern blot probes
Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant
Design Case - ProbeDesign49 - back to list
Summary
./analyse_probe_search_results : Wed Sep 21 14:08:09 2005
Connected to host: host, as user: user Database : southern_blot_designs Fetched probe design: ProbeDesign49 assembly : NCBIM34 bias : 3prime chromosome : 19 id : 31 strand : 1 ------------- Design window length: 2001bp All jobs for probe design are successful - total: 25 Fetched conf : Exonerate mouse_NCBIM34 (id: 1) Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted Fetched 494 putative probes to analyse Selection criteria to rank probes: Minimum score ratio : 10 (i.e. self-hit must score at least 10 times higher than the next best hit) Maximum percent repetitive bases: 5 Favour probes to the 3prime end of design window ------------------------ Putative probes without hits : 0 Putative probes with single_self_hit : 494/494 Putative probes exceeding selection criteria: 286/494 PASSED (GREEN) AND FAILED PUTATIVE PROBES IN THE DESIGN WINDOW --------------------------------------------------------------
Image
Unique (yellow), passed (green) and failed putative probes in the design window
Unique putative probes
No probes
Putative probes
Putative probe ID | Length | Score ratio | Actual | %rep DNA | Distance | Overall |
---|---|---|---|---|---|---|
33517 | 1250 | 39.3 | 6250 | - | 0 | 39.3 |
33530 | 1200 | 37.7 | 6000 | - | 0 | 37.7 |
33531 | 1200 | 37.7 | 6000 | - | 60 | 37.7 |
33506 | 1300 | 40.9 | 6500 | 1.9 | 0 | 37.0 |
33507 | 1300 | 40.9 | 6500 | 1.9 | 65 | 37.0 |
33508 | 1300 | 40.9 | 6500 | 1.9 | 130 | 37.0 |
33509 | 1300 | 40.9 | 6500 | 1.9 | 195 | 37.0 |
33544 | 1150 | 36.2 | 5750 | - | 0 | 36.2 |
33545 | 1150 | 36.2 | 5750 | - | 57 | 36.2 |
33546 | 1150 | 36.2 | 5750 | - | 114 | 36.2 |
33518 | 1250 | 39.3 | 6250 | 2.0 | 62 | 35.3 |
33519 | 1250 | 39.3 | 6250 | 2.0 | 124 | 35.3 |
33520 | 1250 | 39.3 | 6250 | 2.0 | 186 | 35.3 |
33521 | 1250 | 39.3 | 6250 | 2.0 | 248 | 35.3 |
33559 | 1100 | 34.6 | 5500 | - | 0 | 34.6 |
33560 | 1100 | 34.6 | 5500 | - | 55 | 34.6 |
33561 | 1100 | 34.6 | 5500 | - | 110 | 34.6 |
33562 | 1100 | 34.6 | 5500 | - | 165 | 34.6 |
33535 | 1200 | 37.7 | 6000 | 2.1 | 300 | 33.6 |
33576 | 1050 | 33.0 | 5250 | - | 0 | 33.0 |
33577 | 1050 | 33.0 | 5250 | - | 52 | 33.0 |
33578 | 1050 | 33.0 | 5250 | - | 104 | 33.0 |
33579 | 1050 | 33.0 | 5250 | - | 156 | 33.0 |
33580 | 1050 | 33.0 | 5250 | - | 208 | 33.0 |
33595 | 1000 | 31.4 | 5000 | - | 0 | 31.4 |
33596 | 1000 | 31.4 | 5000 | - | 50 | 31.4 |
33597 | 1000 | 31.4 | 5000 | - | 100 | 31.4 |
33598 | 1000 | 31.4 | 5000 | - | 150 | 31.4 |
33599 | 1000 | 31.4 | 5000 | - | 200 | 31.4 |
33600 | 1000 | 31.4 | 5000 | - | 250 | 31.4 |
33616 | 950 | 29.9 | 4750 | - | 0 | 29.9 |
33617 | 950 | 29.9 | 4750 | - | 47 | 29.9 |
33618 | 950 | 29.9 | 4750 | - | 94 | 29.9 |
33619 | 950 | 29.9 | 4750 | - | 141 | 29.9 |
33620 | 950 | 29.9 | 4750 | - | 188 | 29.9 |
33621 | 950 | 29.9 | 4750 | - | 235 | 29.9 |
33622 | 950 | 29.9 | 4750 | - | 282 | 29.9 |
33647 | 900 | 28.5 | 4500 | - | 360 | 28.5 |
33639 | 900 | 28.3 | 4500 | - | 0 | 28.3 |
33640 | 900 | 28.3 | 4500 | - | 45 | 28.3 |
33641 | 900 | 28.3 | 4500 | - | 90 | 28.3 |
33642 | 900 | 28.3 | 4500 | - | 135 | 28.3 |
33643 | 900 | 28.3 | 4500 | - | 180 | 28.3 |
33644 | 900 | 28.3 | 4500 | - | 225 | 28.3 |
33645 | 900 | 28.3 | 4500 | - | 270 | 28.3 |
33646 | 900 | 28.3 | 4500 | - | 315 | 28.3 |
33623 | 950 | 29.9 | 4750 | 0.8 | 329 | 28.2 |
33673 | 850 | 26.9 | 4250 | - | 378 | 26.9 |
33674 | 850 | 26.9 | 4250 | - | 420 | 26.9 |
33664 | 850 | 26.7 | 4250 | - | 0 | 26.7 |
33665 | 850 | 26.7 | 4250 | - | 42 | 26.7 |
33666 | 850 | 26.7 | 4250 | - | 84 | 26.7 |
33667 | 850 | 26.7 | 4250 | - | 126 | 26.7 |
33668 | 850 | 26.7 | 4250 | - | 168 | 26.7 |
33669 | 850 | 26.7 | 4250 | - | 210 | 26.7 |
33670 | 850 | 26.7 | 4250 | - | 252 | 26.7 |
33671 | 850 | 26.7 | 4250 | - | 294 | 26.7 |
33672 | 850 | 26.2 | 4250 | - | 336 | 26.2 |
33585 | 1050 | 33.2 | 5250 | 3.7 | 468 | 25.8 |
33701 | 800 | 25.3 | 4000 | - | 360 | 25.3 |
33702 | 800 | 25.3 | 4000 | - | 400 | 25.3 |
33703 | 800 | 25.3 | 4000 | - | 440 | 25.3 |
33692 | 800 | 25.2 | 4000 | - | 0 | 25.2 |
33693 | 800 | 25.2 | 4000 | - | 40 | 25.2 |
33694 | 800 | 25.2 | 4000 | - | 80 | 25.2 |
33695 | 800 | 25.2 | 4000 | - | 120 | 25.2 |
33696 | 800 | 25.2 | 4000 | - | 160 | 25.2 |
33697 | 800 | 25.2 | 4000 | - | 200 | 25.2 |
33698 | 800 | 25.2 | 4000 | - | 240 | 25.2 |
33699 | 800 | 25.2 | 4000 | - | 280 | 25.2 |
33700 | 800 | 25.2 | 4000 | - | 320 | 25.2 |
33732 | 750 | 23.7 | 3750 | - | 333 | 23.7 |
33733 | 750 | 23.7 | 3750 | - | 370 | 23.7 |
33734 | 750 | 23.7 | 3750 | - | 407 | 23.7 |
33735 | 750 | 23.7 | 3750 | - | 444 | 23.7 |
33736 | 750 | 23.7 | 3750 | - | 481 | 23.7 |
33737 | 750 | 23.7 | 3750 | - | 518 | 23.7 |
33723 | 750 | 23.6 | 3750 | - | 0 | 23.6 |
33724 | 750 | 23.6 | 3750 | - | 37 | 23.6 |
33725 | 750 | 23.6 | 3750 | - | 74 | 23.6 |
33726 | 750 | 23.6 | 3750 | - | 111 | 23.6 |
33727 | 750 | 23.6 | 3750 | - | 148 | 23.6 |
33728 | 750 | 23.6 | 3750 | - | 185 | 23.6 |
33729 | 750 | 23.6 | 3750 | - | 222 | 23.6 |
33730 | 750 | 23.6 | 3750 | - | 259 | 23.6 |
33731 | 750 | 23.6 | 3750 | - | 296 | 23.6 |
33704 | 800 | 25.3 | 4000 | 1.1 | 480 | 23.1 |
33628 | 950 | 30.1 | 4750 | 3.7 | 564 | 22.7 |
33768 | 700 | 22.2 | 3500 | - | 385 | 22.2 |
33769 | 700 | 22.2 | 3500 | - | 420 | 22.2 |
33770 | 700 | 22.2 | 3500 | - | 455 | 22.2 |
33771 | 700 | 22.2 | 3500 | - | 490 | 22.2 |
33772 | 700 | 22.2 | 3500 | - | 525 | 22.2 |
33773 | 700 | 22.2 | 3500 | - | 560 | 22.2 |
33757 | 700 | 22.0 | 3500 | - | 0 | 22.0 |
33758 | 700 | 22.0 | 3500 | - | 35 | 22.0 |
33759 | 700 | 22.0 | 3500 | - | 70 | 22.0 |
33760 | 700 | 22.0 | 3500 | - | 105 | 22.0 |
33761 | 700 | 22.0 | 3500 | - | 140 | 22.0 |
33762 | 700 | 22.0 | 3500 | - | 175 | 22.0 |
33763 | 700 | 22.0 | 3500 | - | 210 | 22.0 |
33764 | 700 | 22.0 | 3500 | - | 245 | 22.0 |
33765 | 700 | 22.0 | 3500 | - | 280 | 22.0 |
33766 | 700 | 22.0 | 3500 | - | 315 | 22.0 |
33767 | 700 | 21.6 | 3500 | - | 350 | 21.6 |
33807 | 650 | 20.6 | 3250 | - | 384 | 20.6 |
33808 | 650 | 20.6 | 3250 | - | 416 | 20.6 |
33809 | 650 | 20.6 | 3250 | - | 448 | 20.6 |
33810 | 650 | 20.6 | 3250 | - | 480 | 20.6 |
33811 | 650 | 20.6 | 3250 | - | 512 | 20.6 |
33812 | 650 | 20.6 | 3250 | - | 544 | 20.6 |
33813 | 650 | 20.6 | 3250 | - | 576 | 20.6 |
33814 | 650 | 20.6 | 3250 | - | 608 | 20.6 |
33795 | 650 | 20.4 | 3250 | - | 0 | 20.4 |
33796 | 650 | 20.4 | 3250 | - | 32 | 20.4 |
33797 | 650 | 20.4 | 3250 | - | 64 | 20.4 |
33798 | 650 | 20.4 | 3250 | - | 96 | 20.4 |
33799 | 650 | 20.4 | 3250 | - | 128 | 20.4 |
33800 | 650 | 20.4 | 3250 | - | 160 | 20.4 |
33801 | 650 | 20.4 | 3250 | - | 192 | 20.4 |
33802 | 650 | 20.4 | 3250 | - | 224 | 20.4 |
33803 | 650 | 20.4 | 3250 | - | 256 | 20.4 |
33804 | 650 | 20.4 | 3250 | - | 288 | 20.4 |
33805 | 650 | 20.4 | 3250 | - | 320 | 20.4 |
33806 | 650 | 20.1 | 3250 | - | 352 | 20.1 |
33850 | 600 | 19.9 | 3000 | - | 360 | 19.9 |
33851 | 600 | 19.0 | 3000 | - | 390 | 19.0 |
33852 | 600 | 19.0 | 3000 | - | 420 | 19.0 |
33853 | 600 | 19.0 | 3000 | - | 450 | 19.0 |
33854 | 600 | 19.0 | 3000 | - | 480 | 19.0 |
33855 | 600 | 19.0 | 3000 | - | 510 | 19.0 |
33856 | 600 | 19.0 | 3000 | - | 540 | 19.0 |
33857 | 600 | 19.0 | 3000 | - | 570 | 19.0 |
33858 | 600 | 19.0 | 3000 | - | 600 | 19.0 |
33859 | 600 | 19.0 | 3000 | - | 630 | 19.0 |
33860 | 600 | 19.0 | 3000 | - | 660 | 19.0 |
33838 | 600 | 18.9 | 3000 | - | 0 | 18.9 |
33839 | 600 | 18.9 | 3000 | - | 30 | 18.9 |
33840 | 600 | 18.9 | 3000 | - | 60 | 18.9 |
33841 | 600 | 18.9 | 3000 | - | 90 | 18.9 |
33842 | 600 | 18.9 | 3000 | - | 120 | 18.9 |
33843 | 600 | 18.9 | 3000 | - | 150 | 18.9 |
33844 | 600 | 18.9 | 3000 | - | 180 | 18.9 |
33845 | 600 | 18.9 | 3000 | - | 210 | 18.9 |
33846 | 600 | 18.9 | 3000 | - | 240 | 18.9 |
33847 | 600 | 18.9 | 3000 | - | 270 | 18.9 |
33848 | 600 | 18.9 | 3000 | - | 300 | 18.9 |
33849 | 600 | 18.9 | 3000 | - | 330 | 18.9 |
33901 | 550 | 17.4 | 2750 | - | 432 | 17.4 |
33902 | 550 | 17.4 | 2750 | - | 459 | 17.4 |
33903 | 550 | 17.4 | 2750 | - | 486 | 17.4 |
33904 | 550 | 17.4 | 2750 | - | 513 | 17.4 |
33905 | 550 | 17.4 | 2750 | - | 540 | 17.4 |
33906 | 550 | 17.4 | 2750 | - | 567 | 17.4 |
33907 | 550 | 17.4 | 2750 | - | 594 | 17.4 |
33908 | 550 | 17.4 | 2750 | - | 621 | 17.4 |
33909 | 550 | 17.4 | 2750 | - | 648 | 17.4 |
33910 | 550 | 17.4 | 2750 | - | 675 | 17.4 |
33911 | 550 | 17.4 | 2750 | - | 702 | 17.4 |
33885 | 550 | 17.3 | 2750 | - | 0 | 17.3 |
33886 | 550 | 17.3 | 2750 | - | 27 | 17.3 |
33887 | 550 | 17.3 | 2750 | - | 54 | 17.3 |
33888 | 550 | 17.3 | 2750 | - | 81 | 17.3 |
33889 | 550 | 17.3 | 2750 | - | 108 | 17.3 |
33890 | 550 | 17.3 | 2750 | - | 135 | 17.3 |
33891 | 550 | 17.3 | 2750 | - | 162 | 17.3 |
33892 | 550 | 17.3 | 2750 | - | 189 | 17.3 |
33893 | 550 | 17.3 | 2750 | - | 216 | 17.3 |
33894 | 550 | 17.3 | 2750 | - | 243 | 17.3 |
33895 | 550 | 17.3 | 2750 | - | 270 | 17.3 |
33896 | 550 | 17.3 | 2750 | - | 297 | 17.3 |
33897 | 550 | 17.3 | 2750 | - | 324 | 17.3 |
33898 | 550 | 17.0 | 2750 | - | 351 | 17.0 |
33958 | 500 | 15.8 | 2500 | - | 475 | 15.8 |
33959 | 500 | 15.8 | 2500 | - | 500 | 15.8 |
33960 | 500 | 15.8 | 2500 | - | 525 | 15.8 |
33961 | 500 | 15.8 | 2500 | - | 550 | 15.8 |
33962 | 500 | 15.8 | 2500 | - | 575 | 15.8 |
33963 | 500 | 15.8 | 2500 | - | 600 | 15.8 |
33964 | 500 | 15.8 | 2500 | - | 625 | 15.8 |
33965 | 500 | 15.8 | 2500 | - | 650 | 15.8 |
33966 | 500 | 15.8 | 2500 | - | 675 | 15.8 |
33967 | 500 | 15.8 | 2500 | - | 700 | 15.8 |
33968 | 500 | 15.8 | 2500 | - | 725 | 15.8 |
33969 | 500 | 15.8 | 2500 | - | 750 | 15.8 |
33939 | 500 | 15.7 | 2500 | - | 0 | 15.7 |
33940 | 500 | 15.7 | 2500 | - | 25 | 15.7 |
33941 | 500 | 15.7 | 2500 | - | 50 | 15.7 |
33942 | 500 | 15.7 | 2500 | - | 75 | 15.7 |
33943 | 500 | 15.7 | 2500 | - | 100 | 15.7 |
33944 | 500 | 15.7 | 2500 | - | 125 | 15.7 |
33945 | 500 | 15.7 | 2500 | - | 150 | 15.7 |
33946 | 500 | 15.7 | 2500 | - | 175 | 15.7 |
33947 | 500 | 15.7 | 2500 | - | 200 | 15.7 |
33948 | 500 | 15.7 | 2500 | - | 225 | 15.7 |
33949 | 500 | 15.7 | 2500 | - | 250 | 15.7 |
33950 | 500 | 15.7 | 2500 | - | 275 | 15.7 |
33951 | 500 | 15.7 | 2500 | - | 300 | 15.7 |
33952 | 500 | 15.7 | 2500 | - | 325 | 15.7 |
33953 | 500 | 15.4 | 2500 | - | 350 | 15.4 |
33780 | 700 | 22.2 | 3500 | 3.7 | 805 | 14.7 |
33912 | 550 | 17.4 | 2750 | 1.5 | 729 | 14.5 |
33970 | 500 | 15.8 | 2500 | 0.8 | 775 | 14.2 |
Overall score = score_ratio - 2 * repetitive DNA content
Redundant probes
Putative probe ID | Length | Score ratio | Actual | %rep DNA | Distance | Overall |
---|---|---|---|---|---|---|
33532 | 1200 | 37.7 | 6000 | 2.1 | 120 | 33.6 |
33533 | 1200 | 37.7 | 6000 | 2.1 | 180 | 33.6 |
33534 | 1200 | 37.7 | 6000 | 2.1 | 240 | 33.6 |
33547 | 1150 | 36.2 | 5750 | 2.2 | 171 | 31.8 |
33548 | 1150 | 36.2 | 5750 | 2.2 | 228 | 31.8 |
33549 | 1150 | 36.2 | 5750 | 2.2 | 285 | 31.8 |
33550 | 1150 | 35.5 | 5750 | 2.2 | 342 | 31.1 |
33566 | 1100 | 34.8 | 5500 | 2.3 | 385 | 30.3 |
33563 | 1100 | 34.6 | 5500 | 2.3 | 220 | 30.0 |
33564 | 1100 | 34.6 | 5500 | 2.3 | 275 | 30.0 |
33565 | 1100 | 34.6 | 5500 | 2.3 | 330 | 30.0 |
33583 | 1050 | 33.2 | 5250 | 2.4 | 364 | 28.5 |
33584 | 1050 | 33.2 | 5250 | 2.4 | 416 | 28.5 |
33581 | 1050 | 33.0 | 5250 | 2.4 | 260 | 28.3 |
33582 | 1050 | 33.0 | 5250 | 2.4 | 312 | 28.3 |
33603 | 1000 | 31.6 | 5000 | 2.5 | 400 | 26.6 |
33604 | 1000 | 31.6 | 5000 | 2.5 | 450 | 26.6 |
33605 | 1000 | 31.6 | 5000 | 2.5 | 500 | 26.6 |
33601 | 1000 | 31.4 | 5000 | 2.5 | 300 | 26.4 |
33602 | 1000 | 30.9 | 5000 | 2.5 | 350 | 25.9 |
33624 | 950 | 30.1 | 4750 | 2.6 | 376 | 24.8 |
33625 | 950 | 30.1 | 4750 | 2.6 | 423 | 24.8 |
33626 | 950 | 30.1 | 4750 | 2.6 | 470 | 24.8 |
33627 | 950 | 30.1 | 4750 | 2.6 | 517 | 24.8 |
33648 | 900 | 28.5 | 4500 | 2.8 | 405 | 22.9 |
33649 | 900 | 28.5 | 4500 | 2.8 | 450 | 22.9 |
33650 | 900 | 28.5 | 4500 | 2.8 | 495 | 22.9 |
33651 | 900 | 28.5 | 4500 | 2.8 | 540 | 22.9 |
33652 | 900 | 28.5 | 4500 | 2.8 | 585 | 22.9 |
33675 | 850 | 26.9 | 4250 | 2.9 | 462 | 21.0 |
33676 | 850 | 26.9 | 4250 | 2.9 | 504 | 21.0 |
33677 | 850 | 26.9 | 4250 | 2.9 | 546 | 21.0 |
33678 | 850 | 26.9 | 4250 | 2.9 | 588 | 21.0 |
33679 | 850 | 26.9 | 4250 | 2.9 | 630 | 21.0 |
33705 | 800 | 25.3 | 4000 | 3.1 | 520 | 19.1 |
33706 | 800 | 25.3 | 4000 | 3.1 | 560 | 19.1 |
33707 | 800 | 25.3 | 4000 | 3.1 | 600 | 19.1 |
33708 | 800 | 25.3 | 4000 | 3.1 | 640 | 19.1 |
33709 | 800 | 25.3 | 4000 | 3.1 | 680 | 19.1 |
33738 | 750 | 23.7 | 3750 | 3.3 | 555 | 17.1 |
33739 | 750 | 23.7 | 3750 | 3.3 | 592 | 17.1 |
33740 | 750 | 23.7 | 3750 | 3.3 | 629 | 17.1 |
33741 | 750 | 23.7 | 3750 | 3.3 | 666 | 17.1 |
33742 | 750 | 23.7 | 3750 | 3.3 | 703 | 17.1 |
33743 | 750 | 23.7 | 3750 | 3.3 | 740 | 17.1 |
33774 | 700 | 22.2 | 3500 | 3.4 | 595 | 15.3 |
33775 | 700 | 22.2 | 3500 | 3.6 | 630 | 15.0 |
33776 | 700 | 22.2 | 3500 | 3.6 | 665 | 15.0 |
33777 | 700 | 22.2 | 3500 | 3.6 | 700 | 15.0 |
33778 | 700 | 22.2 | 3500 | 3.6 | 735 | 15.0 |
33779 | 700 | 22.2 | 3500 | 3.6 | 770 | 15.0 |
33815 | 650 | 20.6 | 3250 | 2.9 | 640 | 14.7 |
33816 | 650 | 20.6 | 3250 | 3.8 | 672 | 12.9 |
33817 | 650 | 20.6 | 3250 | 3.8 | 704 | 12.9 |
33818 | 650 | 20.6 | 3250 | 3.8 | 736 | 12.9 |
33819 | 650 | 20.6 | 3250 | 3.8 | 768 | 12.9 |
33820 | 650 | 20.6 | 3250 | 3.8 | 800 | 12.9 |
33821 | 650 | 20.6 | 3250 | 3.8 | 832 | 12.9 |
33861 | 600 | 19.0 | 3000 | 3.2 | 690 | 12.7 |
33862 | 600 | 19.0 | 3000 | 4.2 | 720 | 10.7 |
33863 | 600 | 19.0 | 3000 | 4.2 | 750 | 10.7 |
33864 | 600 | 19.0 | 3000 | 4.2 | 780 | 10.7 |
33865 | 600 | 19.0 | 3000 | 4.2 | 810 | 10.7 |
33866 | 600 | 19.0 | 3000 | 4.2 | 840 | 10.7 |
33867 | 600 | 19.0 | 3000 | 4.2 | 870 | 10.7 |
33868 | 600 | 19.0 | 3000 | 4.2 | 900 | 10.7 |
33919 | 550 | 17.9 | 2750 | 4.5 | 918 | 8.8 |
33920 | 550 | 17.9 | 2750 | 4.5 | 945 | 8.8 |
33913 | 550 | 17.4 | 2750 | 4.5 | 756 | 8.3 |
33914 | 550 | 17.4 | 2750 | 4.5 | 783 | 8.3 |
33915 | 550 | 17.4 | 2750 | 4.5 | 810 | 8.3 |
33916 | 550 | 17.4 | 2750 | 4.5 | 837 | 8.3 |
33917 | 550 | 17.4 | 2750 | 4.5 | 864 | 8.3 |
33918 | 550 | 17.4 | 2750 | 4.5 | 891 | 8.3 |
33979 | 500 | 16.4 | 2500 | 5.0 | 1000 | 6.4 |
33976 | 500 | 16.2 | 2500 | 5.0 | 925 | 6.2 |
33977 | 500 | 16.2 | 2500 | 5.0 | 950 | 6.2 |
33978 | 500 | 16.2 | 2500 | 5.0 | 975 | 6.2 |
33971 | 500 | 15.8 | 2500 | 5.0 | 800 | 5.8 |
33972 | 500 | 15.8 | 2500 | 5.0 | 825 | 5.8 |
33973 | 500 | 15.8 | 2500 | 5.0 | 850 | 5.8 |
33974 | 500 | 15.8 | 2500 | 5.0 | 875 | 5.8 |
33975 | 500 | 15.8 | 2500 | 5.0 | 900 | 5.8 |
Overall score = score_ratio - 2 * repetitive DNA content