Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign37 - back to list

Summary

./analyse_probe_search_results : Mon Sep 5 11:33:34 2005

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign37
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : X
  id            : 13
  strand        : 1
  -------------

Design window length: 18537bp

All jobs for probe design are successful - total: 363
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 7251 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                : 0
Putative probes with single_self_hit        : 7251/7251
Putative probes exceeding selection criteria: 806/7251

PASSED (GREEN) AND FAILED PUTATIVE PROBES IN THE DESIGN WINDOW
--------------------------------------------------------------

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Chart

Unique putative probes

No probes

Putative probes

Putative probe IDLengthScore ratioActual%rep DNADistanceOverall
11631100033.35000-1740033.3
11632100033.35000-1745033.3
11633100033.35000-1750033.3
1200495031.74750-1739031.7
1200595031.74750-1743731.7
1200695031.74750-1748431.7
1200795031.74750-1753131.7
1200895031.74750-1757831.7
11630100031.65000-1735031.6
11328100032.550001.0225030.5
1168395030.847500.2230330.4
11320100032.350001.0185030.3
11321100032.350001.0190030.3
11322100032.350001.0195030.3
11323100032.350001.0200030.3
11324100032.350001.0205030.3
11325100032.350001.0210030.3
11326100032.350001.0215030.3
11327100032.350001.0220030.3
1200395030.14750-1734330.1
1239590030.04500-1737030.0
1239690030.04500-1741530.0
1239790030.04500-1746030.0
1239890030.04500-1750530.0
1239990030.04500-1755030.0
1240090030.04500-1759530.0
1168295030.847500.5225629.8
1206090029.24500-229529.2
1206190029.24500-234029.2
11629100028.95000-1730028.9
1167395030.647501.1183328.5
1281585028.34250-1738828.3
1281685028.34250-1743028.3
1281785028.34250-1747228.3
1281885028.34250-1751428.3
1281985028.34250-1755628.3
1282085028.34250-1759828.3
11319100032.350002.1180028.1
1245585027.64250-226827.6
1245685027.64250-231027.6
1245785027.64250-235227.6
1245885027.64250-239427.6
1200295027.54750-1729627.5
1239490027.44500-1732527.4
1281485026.94250-1734626.9
1205090029.045001.1184526.8
1205190029.045001.1189026.8
1325780026.74000-1736026.7
1325880026.74000-1740026.7
1325980026.74000-1744026.7
1326080026.74000-1748026.7
1326180026.74000-1752026.7
1326280026.74000-1756026.7
1326380026.74000-1760026.7
1288180026.34000-232026.3
1288080026.04000-228026.0
1288280026.04000-236026.0
1288380026.04000-240026.0
1288480026.04000-244026.0
1244585027.442501.2184825.1
1244685027.442501.2189025.1
1244785027.442501.2193225.1
1373675025.03750-1735325.0
1373775025.03750-1739025.0
1373875025.03750-1742725.0
1373975025.03750-1746425.0
1374075025.03750-1750125.0
1374175025.03750-1753825.0
1374275025.03750-1757525.0
1333075024.73750-233124.7
1333175024.73750-236824.7
1281385024.64250-1730424.6
1332975024.43750-229424.4
1333275024.43750-240524.4
1333375024.43750-244224.4
1333475024.43750-247924.4
1244485027.442501.8180623.9
1424470023.33500-1736023.3
1424570023.33500-1739523.3
1424670023.33500-1743023.3
1424770023.33500-1746523.3
1424870023.33500-1750023.3
1424970023.33500-1753523.3
1425070023.33500-1757023.3
1286980025.840001.2184023.3
1287080025.840001.2188023.3
1287180025.840001.2192023.3
1287280025.840001.2196023.3
1287380025.840001.2200023.3
1332875024.437500.5225723.3
1167295030.647503.7178623.3
1325680023.14000-1732023.1
1381470023.03500-231023.0
1381570023.03500-234523.0
1381670023.03500-238023.0
1381770023.03500-241523.0
1414470023.235000.11386022.9
1381370022.73500-227522.7
1381870022.73500-245022.7
1381970022.73500-248522.7
1382070022.73500-252022.7
11329100032.550004.9230022.7
1390670022.43500-553022.4
1390770022.43500-556522.4
1341675022.23750-551322.2
1239390025.745001.81728022.2
1412170022.23500-1305522.2
1390570022.03500-549522.0
1412070022.235000.11302021.9
1373575021.73750-1731621.7
1480165021.73250-1737621.7
1480265021.73250-1740821.7
1480365021.73250-1744021.7
1480465021.73250-1747221.7
1480565021.73250-1750421.7
1480665021.73250-1753621.7
1480765021.73250-1756821.7
1480865021.73250-1760021.7
1206290029.245003.8238521.7
1382170022.735000.6255521.6
1329375021.63750-96221.6
1331675024.237501.3181321.5
1331775024.237501.3185021.5
1331875024.237501.3188721.5
1331975024.237501.3192421.5
1332075024.237501.3196121.5
1332175024.237501.3199821.5
1332275024.237501.3203521.5
1469265021.53250-1388821.5
1361975021.43750-1302421.4
1433165021.43250-233621.4
1433265021.43250-236821.4
1433365021.43250-240021.4
1433465021.43250-243221.4
1433565021.43250-246421.4
1433665021.43250-249621.4
1424370021.33500-1732521.3
1432965021.13250-227221.1
1433065021.13250-230421.1
1433765021.13250-252821.1
1433865021.13250-256021.1
1433965021.13250-259221.1
1443165020.83250-553620.8
1443265020.83250-556820.8
1443365020.83250-560020.8
1469165021.532500.51385620.6
1466565020.63250-1302420.6
1466665020.63250-1305620.6
1466765020.63250-1308820.6
1480065020.63250-1734420.6
1443065020.43250-550420.4
1333575024.437502.0251620.4
1377570020.13500-94520.1
1377670020.13500-98020.1
1377770020.13500-101520.1
1412270020.03500-1309020.0
1539660020.03000-1737020.0
1539760020.03000-1740020.0
1539860020.03000-1743020.0
1539960020.03000-1746020.0
1540060020.03000-1749020.0
1540160020.03000-1752020.0
1540260020.03000-1755020.0
1540360020.03000-1758020.0
1527960019.93000-1386019.9
1528060019.93000-1389019.9
1528160019.93000-1392019.9
1528260019.93000-1395019.9
1489460019.73000-231019.7
1489560019.73000-234019.7
1489660019.73000-237019.7
1489760019.73000-240019.7
1489860019.73000-243019.7
1489960019.73000-246019.7
1490060019.73000-249019.7
1490160019.73000-252019.7
1380070022.635001.4182019.7
1380170022.635001.4185519.7
1380270022.635001.4189019.7
1380370022.635001.4192519.7
1380470022.635001.4196019.7
1380570022.635001.4199519.7
1380670022.635001.4203019.7
1380770022.635001.4206519.7
1380870022.635001.4210019.7
1404970021.935001.11053519.6
1489360019.53000-228019.5
1490260019.53000-255019.5
1490360019.53000-258019.5
1490460019.53000-261019.5
1490560019.53000-264019.5
1525560019.53000-1314019.5
1500660019.730000.2567019.4
1245985027.642504.1243619.4
1500260019.23000-555019.2
1500360019.23000-558019.2
1500460019.23000-561019.2
1500560019.23000-564019.2
1525260019.03000-1305019.0
1525360019.03000-1308019.0
1525460019.03000-1311019.0
1539560019.03000-1734019.0
1296080025.240003.1548018.9
1325580022.940002.01728018.9
1479965018.83250-1731218.8
1288580026.040003.6248018.7
1428865018.73250-96018.7
1428965018.73250-99218.7
1429065018.73250-102418.7
1429165018.73250-105618.7
1525160019.030000.21302018.7
1466865018.63250-1312018.6
1500160018.53000-552018.5
1424270020.235000.91729018.5
1605855018.32750-1736118.3
1605955018.32750-1738818.3
1606055018.32750-1741518.3
1606155018.32750-1744218.3
1606255018.32750-1746918.3
1606355018.32750-1749618.3
1606455018.32750-1752318.3
1606555018.32750-1755018.3
1606655018.32750-1757718.3
1592955018.22750-1387818.2
1593055018.22750-1390518.2
1593155018.22750-1393218.2
1593255018.22750-1395918.2
1593355018.22750-1398618.2
1469365021.532501.71392018.1
1550155018.12750-232218.1
1550255018.12750-234918.1
1550355018.12750-237618.1
1550455018.12750-240318.1
1550555018.12750-243018.1
1550655018.12750-245718.1
1550755018.12750-248418.1
1550855018.12750-251118.1
1550955018.12750-253818.1
1551055018.12750-256518.1
1551155018.12750-259218.1
1562555018.12750-567018.1
1562655018.12750-569718.1
1590155018.12750-1312218.1
1590255018.12750-1314918.1
1590355018.12750-1317618.1
1431565021.032501.5182417.9
1431665021.032501.5185617.9
1431765021.032501.5188817.9
1431865021.032501.5192017.9
1431965021.032501.5195217.9
1432065021.032501.5198417.9
1432165021.032501.5201617.9
1432265021.032501.5204817.9
1432365021.032501.5208017.9
1432465021.032501.5211217.9
1432565021.032501.5214417.9
1549955017.92750-226817.9
1550055017.92750-229517.9
1551255017.92750-261917.9
1551355017.92750-264617.9
1551455017.92750-267317.9
1551555017.92750-270017.9
1590455017.92750-1320317.9
1458765020.332501.21052817.9
1458865020.332501.21056017.9
1458965020.332501.21059217.9
1562055017.62750-553517.6
1562155017.62750-556217.6
1562255017.62750-558917.6
1562355017.62750-561617.6
1562455017.62750-564317.6
1500060018.930000.7549017.5
1589855017.42750-1304117.4
1589955017.42750-1306817.4
1590055017.42750-1309517.4
1539460017.33000-1731017.3
1484960017.23000-96017.2
1485060017.23000-99017.2
1485160017.23000-102017.2
1485260017.23000-105017.2
1485360017.23000-108017.2
1485460017.23000-111017.2
1362075021.437502.11306117.2
1525660017.13000-1317017.1
1605755017.12750-1733417.1
1443465020.832502.0563216.8
1593455018.227500.71401316.8
1329275021.637502.492516.8
1677750016.72500-1737516.7
1677850016.72500-1740016.7
1677950016.72500-1742516.7
1678050016.72500-1745016.7
1678150016.72500-1747516.7
1678250016.72500-1750016.7
1678350016.72500-1752516.7
1678450016.72500-1755016.7
1678550016.72500-1757516.7
1678650016.72500-1760016.7
1663750016.62500-1387516.6
1663850016.62500-1390016.6
1663950016.62500-1392516.6
1664050016.62500-1395016.6
1664150016.62500-1397516.6
1664250016.62500-1400016.6
1341575021.337502.4547616.5
1617550016.42500-232516.4
1617650016.42500-235016.4
1617750016.42500-237516.4
1617850016.42500-240016.4
1617950016.42500-242516.4
1618050016.42500-245016.4
1618150016.42500-247516.4
1618250016.42500-250016.4
1618350016.42500-252516.4
1618450016.42500-255016.4
1618550016.42500-257516.4
1618650016.42500-260016.4
1618750016.42500-262516.4
1630850016.42500-565016.4
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1631050016.42500-570016.4
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1661050016.42500-1320016.4
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1466965018.632501.11315216.4
1314880022.940003.21300016.4
1373475020.837502.31727916.3
1562755018.127500.9572416.3
1561955016.32750-550816.3
1284680023.040003.492016.2
1617350016.22500-227516.2
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1619050016.22500-270016.2
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1619250016.22500-275016.2
1661250016.22500-1325016.2
1516860018.830001.31053016.1
1516960018.830001.31056016.1
1517060018.830001.31059016.1
1517160018.830001.31062016.1
1630450016.02500-555016.0
1630550016.02500-557516.0
1630650016.02500-560016.0
1630750016.02500-562516.0
1487860019.430001.7183016.0
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1488060019.430001.7189016.0
1488160019.430001.7192016.0
1488260019.430001.7195016.0
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1850640013.32000-1758013.3
1850740013.32000-1760013.3
1832040013.22000-1386013.2
1832140013.22000-1388013.2
1832240013.22000-1390013.2
1832340013.22000-1392013.2
1832440013.22000-1394013.2
1832540013.22000-1396013.2
1832640013.22000-1398013.2
1832740013.22000-1400013.2
1774440013.22000-234013.2
1774540013.22000-236013.2
1774640013.22000-238013.2
1774740013.22000-240013.2
1774840013.22000-242013.2
1774940013.22000-244013.2
1775040013.22000-246013.2
1775140013.22000-248013.2
1775240013.22000-250013.2
1775340013.22000-252013.2
1775440013.22000-254013.2
1775540013.22000-256013.2
1775640013.22000-258013.2
1775740013.22000-260013.2
1775840013.22000-262013.2
1775940013.22000-264013.2
1791440013.22000-574013.2
1791540013.22000-576013.2
1791640013.22000-578013.2
1791740013.22000-580013.2
1791840013.22000-582013.2
1791940013.22000-584013.2
1792040013.22000-586013.2
1828440013.22000-1314013.2
1828540013.22000-1316013.2
1828640013.22000-1318013.2
1828740013.22000-1320013.2
1828840013.22000-1322013.2
1828940013.22000-1324013.2
1829040013.22000-1326013.2
1829140013.22000-1328013.2
1829240013.22000-1330013.2
1829340013.22000-1332013.2
1490660019.530003.2267013.1
1650850016.325001.61065013.1
1650950016.325001.61067513.1
1651050016.325001.61070013.1
1651150016.325001.61072513.1
1759145013.02250-1731413.0
1774140013.02000-228013.0
1774240013.02000-230013.0
1776540013.02000-276013.0
1776640013.02000-278013.0
1776740013.02000-280013.0
1776840013.02000-282013.0
1776940013.02000-284013.0
1829440013.02000-1334013.0
1663650016.625001.81385013.0
1684745012.92250-94612.9
1684845012.92250-96812.9
1684945012.92250-99012.9
1685045012.92250-101212.9
1685145012.92250-103412.9
1685245012.92250-105612.9
1685345012.92250-107812.9
1685445012.92250-110012.9
1685545012.92250-112212.9
1685645012.92250-114412.9
1685745012.92250-116612.9
1685845012.92250-118812.9
1740945012.92250-1331012.9
1484860017.230002.293012.9
1528360019.930003.51398012.9
1741045012.92250-1333212.9
1790440012.82000-554012.8
1790540012.82000-556012.8
1790640012.82000-558012.8
1790740012.82000-560012.8
1790840012.82000-562012.8
1790940012.82000-564012.8
1329475021.637504.499912.8
1827940012.72000-1304012.7
1828040012.72000-1306012.7
1828140012.72000-1308012.7
1828240012.72000-1310012.7
1849440012.72000-1734012.7
1790240012.62000-550012.6
1561855017.327502.4548112.6
1815340012.52000-1052012.5
1815440012.52000-1054012.5
1815540012.52000-1056012.5
1815640012.52000-1058012.5
1774040013.020000.2226012.5
1650350015.625001.61052512.4
1650450015.625001.61055012.4
1650550015.625001.61057512.4
1650650015.625001.61060012.4
1650750015.625001.61062512.4
1790340012.32000-552012.3
1661450014.325001.01330012.3
1617250016.225002.0225012.2
1827840012.720000.21302012.2
1615650016.125002.0185012.1
1615750016.125002.0187512.1
1615850016.125002.0190012.1
1615950016.125002.0192512.1
1616050016.125002.0195012.1
1616150016.125002.0197512.1
1616250016.125002.0200012.1
1616350016.125002.0202512.1
1616450016.125002.0205012.1
1616550016.125002.0207512.1
1616650016.125002.0210012.1
1616750016.125002.0212512.1
1616850016.125002.0215012.1
1616950016.125002.0217512.1
1617050016.125002.0220012.1
1617150016.125002.0222512.1
1429265018.732503.4108811.9
1361875020.937504.51298711.9
1539360017.130002.71728011.8
1458665020.332504.31049611.7
1466465020.632504.51299211.6
1849240011.62000-1730011.6
1849340011.62000-1732011.6
1414370021.235004.91382511.5
1767540011.52000-96011.5
1767640011.52000-98011.5
1767740011.52000-100011.5
1767840011.52000-102011.5
1767940011.52000-104011.5
1768040011.52000-106011.5
1768140011.52000-108011.5
1768240011.52000-110011.5
1768340011.52000-112011.5
1768440011.52000-114011.5
1768540011.52000-116011.5
1768640011.52000-118011.5
1829540011.52000-1336011.5
1829640011.42000-1338011.4
1759045013.022500.91729211.2
1728845014.722501.81064811.2
1728945014.722501.81067011.2
1729045014.722501.81069211.2
1729145014.722501.81071411.2
1729245014.722501.81073611.2
1729345014.722501.81075811.2
1729445014.722501.81078011.2
1377470020.135004.791010.7
1442965017.432503.4547210.6
1728445014.122501.81056010.5
1728545014.122501.81058210.5
1728645014.122501.81060410.5
1728745014.122501.81062610.5
1377870020.135004.9105010.4
1412370018.635004.31312510.0
1767440011.520000.894010.0
1651250016.325003.2107509.9
1706945014.822502.458309.9
1605555015.727502.9172809.9
11628100018.950004.5172509.9
1485560017.230004.011409.2
1815940013.120002.0106409.1
1816040013.120002.0106609.1
1816140013.120002.0106809.1
1816240013.120002.0107009.1
1816340013.120002.0107209.1
1816440013.120002.0107409.1
1816540013.120002.0107609.1
1816640013.120002.0107809.1
1816740013.120002.0108009.1
1816840013.120002.0108209.1
1816940013.120002.0108409.1
1829740011.420001.2134008.9
1741145012.922502.0133548.9
1637050015.725003.672008.5
1527860018.230004.8138308.5
1815740012.520002.0106008.5
1815840012.520002.0106208.5
1777040013.020002.228608.5
1551655017.927504.727278.4
1200195017.947504.8172498.2
1281285016.042504.0172628.0
1693245014.622503.328167.9
1545955015.827504.011887.8
1792140013.220002.858807.7
1717245012.822502.780967.5
1544955015.827504.59186.7
1729545014.722504.0108026.7
1619350016.225004.827756.6
1717145012.822503.180746.6
1803140011.420002.580806.4
1803240011.420002.581006.4
1803340011.420002.581206.4
1803440011.420002.581406.4
1630150013.825003.854756.2
1390470015.335004.954605.6
1743345013.622504.7138384.3
1728145014.122504.9104944.3
1677350012.525004.2172754.1
1849140011.420004.0172803.4
1831940012.120004.8138402.6
1803040011.420004.580602.4

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe IDLengthScore ratioActual%rep DNADistanceOverall
1167495030.647501.1188028.5
1167595030.647501.1192728.5
1167695030.647501.1197428.5
1167795030.647501.1202128.5
1167895030.647501.1206828.5
1167995030.647501.1211528.5
1168095030.647501.1216228.5
1168195030.647501.1220928.5
1205990029.245001.1225027.0
1205290029.045001.1193526.8
1205390029.045001.1198026.8
1205490029.045001.1202526.8
1205590029.045001.1207026.8
1205690029.045001.1211526.8
1205790029.045001.1216026.8
1205890029.045001.1220526.8
1244885027.442501.2197425.1
1244985027.442501.2201625.1
1245085027.442501.2205825.1
1245185027.442501.2210025.1
1245285027.442501.2214225.1
1245385027.442501.2218425.1
1245485027.442501.2222625.1
1204990029.045002.3180024.4
1287480025.840001.2204023.3
1287580025.840001.2208023.3
1287680025.840001.2212023.3
1287780025.840001.2216023.3
1287880025.840001.2220023.3
1287980025.840001.2224023.3
1332375024.237501.3207221.5
1332475024.237501.3210921.5
1332575024.237501.3214621.5
1332675024.237501.3218321.5
1332775024.237501.3222021.5
1286880025.840002.6180020.6
1380970022.635001.4213519.7
1381070022.635001.4217019.7
1381170022.635001.4220519.7
1381270022.635001.4224019.7
1432665021.032501.5217617.9
1432765021.032501.5220817.9
1432865021.032501.5224017.9
1489260019.530001.7225016.1
1489160019.430001.7222016.0
1390870022.435004.4560013.6
1548255017.727502.2180913.4
1487760019.430003.5180012.4
1431465021.032504.5179212.0
1590655015.727502.21325711.4
1516760018.830004.01050010.8
1689145014.522502.2191410.1
1689245014.522502.2193610.1
1689345014.522502.2195810.1
1689445014.522502.2198010.1
1689545014.522502.2200210.1
1689645014.522502.2202410.1
1689745014.522502.2204610.1
1689845014.522502.2206810.1
1689945014.522502.2209010.1
1690045014.522502.2211210.1
1690145014.522502.2213410.1
1690245014.522502.2215610.1
1690345014.522502.2217810.1
1690445014.522502.2220010.1
1690545014.522502.2222210.1
1690645014.522502.2224410.1
1580455017.227503.8105039.6
1772540012.920002.519607.9
1772640012.920002.519807.9
1772740012.920002.520007.9
1772840012.920002.520207.9
1772940012.920002.520407.9
1773040012.920002.520607.9
1773140012.920002.520807.9
1773240012.920002.521007.9
1773340012.920002.521207.9
1773440012.920002.521407.9
1773540012.920002.521607.9
1773640012.920002.521807.9
1773740012.920002.522007.9
1773840012.920002.522207.9
1773940012.920002.522407.9
1660250015.825004.2130007.4
1611950014.425003.69257.2
1705345013.622503.654786.4
1650250015.625004.8105006.0
1739545014.222504.2130025.8
1790140012.620003.554805.6
1815240012.520004.0105004.5
1684645012.922504.29244.5
1798740012.620004.572003.6

Overall score = score_ratio - 2 * repetitive DNA content

© G2C 2014. The Genes to Cognition Programme received funding from The Wellcome Trust and the EU FP7 Framework Programmes:
EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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