Automated design of genomic Southern blot probes
Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant
Design Case - ProbeDesign20 - back to list
Summary
./analyse_probe_search_results : Tue Dec 19 12:00:58 2006
Connected to host: host, as user: user Database : southern_blot_designs Fetched probe design: ProbeDesign20 assembly : NCBIM36 bias : 5prime chromosome : 16 id : 2 strand : 1 ------------- Design window length: 3171bp All jobs for probe design are successful - total: 49 Fetched conf : Exonerate mouse_NCBIM36 (id: 1) Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted Fetched 974 putative probes to analyse Selection criteria to rank probes: Minimum score ratio : 10 (i.e. self-hit must score at least 10 times higher than the next best hit) Maximum percent repetitive bases: 5 Favour probes to the 5prime end of design window ------------------------ Putative probes without hits : 0 Putative probes with single_self_hit : 974/974 Putative probes that are unique : 49/974 Putative probes exceeding selection criteria : 581/974 (both unique and not unique) Putative probes with successfully-picked primers: 500/581 Identified 301 'redundant' probes exceeding selection criteria (but wholly contained by other probes, and higher repetitive DNA content)
Image
Unique (yellow), passed (green) and failed putative probes in the design window
Unique putative probes
Putative probe ID | Length | Actual | %rep DNA | Distance |
---|---|---|---|---|
1770 | 700 | 3500 | - | 1260 |
1771 | 700 | 3500 | - | 1295 |
1808 | 650 | 3250 | - | 96 |
1809 | 650 | 3250 | - | 128 |
1845 | 650 | 3250 | - | 1280 |
1846 | 650 | 3250 | - | 1312 |
1847 | 650 | 3250 | - | 1344 |
1887 | 600 | 3000 | - | 90 |
1888 | 600 | 3000 | - | 120 |
1889 | 600 | 3000 | - | 150 |
1890 | 600 | 3000 | - | 180 |
1926 | 600 | 3000 | - | 1260 |
1927 | 600 | 3000 | - | 1290 |
1928 | 600 | 3000 | - | 1320 |
1929 | 600 | 3000 | - | 1350 |
1930 | 600 | 3000 | - | 1380 |
1973 | 550 | 2750 | - | 81 |
1974 | 550 | 2750 | - | 108 |
1975 | 550 | 2750 | - | 135 |
1976 | 550 | 2750 | - | 162 |
1977 | 550 | 2750 | - | 189 |
1978 | 550 | 2750 | - | 216 |
2017 | 550 | 2750 | - | 1269 |
2018 | 550 | 2750 | - | 1296 |
2019 | 550 | 2750 | - | 1323 |
2020 | 550 | 2750 | - | 1350 |
2021 | 550 | 2750 | - | 1377 |
2022 | 550 | 2750 | - | 1404 |
2023 | 550 | 2750 | - | 1431 |
2072 | 500 | 2500 | - | 100 |
2073 | 500 | 2500 | - | 125 |
2074 | 500 | 2500 | - | 150 |
2075 | 500 | 2500 | - | 175 |
2076 | 500 | 2500 | - | 200 |
2077 | 500 | 2500 | - | 225 |
2078 | 500 | 2500 | - | 250 |
2079 | 500 | 2500 | - | 275 |
2118 | 500 | 2500 | - | 1250 |
2119 | 500 | 2500 | - | 1275 |
2120 | 500 | 2500 | - | 1300 |
2121 | 500 | 2500 | - | 1325 |
2122 | 500 | 2500 | - | 1350 |
2123 | 500 | 2500 | - | 1375 |
2124 | 500 | 2500 | - | 1400 |
2125 | 500 | 2500 | - | 1425 |
2126 | 500 | 2500 | - | 1450 |
2127 | 500 | 2500 | - | 1475 |
2128 | 500 | 2500 | - | 1500 |
2174 | 500 | 2500 | - | 2650 |
Putative probes
Putative probe ID | Length | Score ratio | Actual | %rep DNA | Distance | Overall |
---|---|---|---|---|---|---|
1455 | 950 | 28.8 | 4750 | - | 94 | 28.8 |
1503 | 900 | 28.5 | 4500 | - | 90 | 28.5 |
1504 | 900 | 27.3 | 4500 | - | 135 | 27.3 |
1505 | 900 | 27.3 | 4500 | 0.1 | 180 | 27.1 |
1554 | 850 | 26.9 | 4250 | - | 84 | 26.9 |
1555 | 850 | 26.9 | 4250 | - | 126 | 26.9 |
1556 | 850 | 26.9 | 4250 | - | 168 | 26.9 |
1410 | 1000 | 26.3 | 5000 | - | 50 | 26.3 |
1456 | 950 | 28.8 | 4750 | 1.3 | 141 | 26.3 |
1368 | 1050 | 26.0 | 5250 | - | 0 | 26.0 |
1201 | 1300 | 29.0 | 6500 | 1.5 | 0 | 25.9 |
1202 | 1300 | 29.0 | 6500 | 1.5 | 65 | 25.9 |
1203 | 1300 | 29.0 | 6500 | 1.5 | 130 | 25.9 |
1204 | 1300 | 29.0 | 6500 | 1.5 | 195 | 25.9 |
1205 | 1300 | 29.0 | 6500 | 1.5 | 260 | 25.9 |
1206 | 1300 | 29.0 | 6500 | 1.5 | 325 | 25.9 |
1207 | 1300 | 29.0 | 6500 | 1.5 | 390 | 25.9 |
1208 | 1300 | 29.0 | 6500 | 1.5 | 455 | 25.9 |
1209 | 1300 | 29.0 | 6500 | 1.5 | 520 | 25.9 |
1210 | 1300 | 29.0 | 6500 | 1.5 | 585 | 25.9 |
1211 | 1300 | 29.0 | 6500 | 1.5 | 650 | 25.9 |
1212 | 1300 | 29.0 | 6500 | 1.5 | 715 | 25.9 |
1557 | 850 | 25.8 | 4250 | - | 210 | 25.8 |
1610 | 800 | 25.3 | 4000 | - | 80 | 25.3 |
1611 | 800 | 25.3 | 4000 | - | 120 | 25.3 |
1612 | 800 | 25.3 | 4000 | - | 160 | 25.3 |
1613 | 800 | 25.3 | 4000 | - | 200 | 25.3 |
1454 | 950 | 25.0 | 4750 | - | 47 | 25.0 |
1409 | 1000 | 24.8 | 5000 | - | 0 | 24.8 |
1231 | 1250 | 27.9 | 6250 | 1.6 | 62 | 24.7 |
1232 | 1250 | 27.9 | 6250 | 1.6 | 124 | 24.7 |
1233 | 1250 | 27.9 | 6250 | 1.6 | 186 | 24.7 |
1234 | 1250 | 27.9 | 6250 | 1.6 | 248 | 24.7 |
1614 | 800 | 24.2 | 4000 | - | 240 | 24.2 |
1702 | 750 | 24.0 | 3750 | - | 1258 | 24.0 |
1615 | 800 | 24.2 | 4000 | 0.1 | 280 | 24.0 |
1670 | 750 | 23.7 | 3750 | - | 74 | 23.7 |
1671 | 750 | 23.7 | 3750 | - | 111 | 23.7 |
1672 | 750 | 23.7 | 3750 | - | 148 | 23.7 |
1673 | 750 | 23.7 | 3750 | - | 185 | 23.7 |
1674 | 750 | 23.7 | 3750 | - | 222 | 23.7 |
1675 | 750 | 23.7 | 3750 | - | 259 | 23.7 |
1453 | 950 | 23.5 | 4750 | - | 0 | 23.5 |
1502 | 900 | 23.4 | 4500 | - | 45 | 23.4 |
1676 | 750 | 22.7 | 3750 | - | 296 | 22.7 |
1501 | 900 | 22.3 | 4500 | - | 0 | 22.3 |
1737 | 700 | 22.2 | 3500 | - | 105 | 22.2 |
1738 | 700 | 22.2 | 3500 | - | 140 | 22.2 |
1739 | 700 | 22.2 | 3500 | - | 175 | 22.2 |
1740 | 700 | 22.2 | 3500 | - | 210 | 22.2 |
1741 | 700 | 22.2 | 3500 | - | 245 | 22.2 |
1742 | 700 | 22.2 | 3500 | - | 280 | 22.2 |
1743 | 700 | 22.2 | 3500 | - | 315 | 22.2 |
1553 | 850 | 22.1 | 4250 | - | 42 | 22.1 |
1736 | 700 | 21.7 | 3500 | - | 70 | 21.7 |
1844 | 650 | 21.7 | 3250 | - | 1248 | 21.7 |
1677 | 750 | 22.7 | 3750 | 0.5 | 333 | 21.7 |
1744 | 700 | 21.2 | 3500 | - | 350 | 21.2 |
1552 | 850 | 21.0 | 4250 | - | 0 | 21.0 |
1810 | 650 | 20.6 | 3250 | - | 160 | 20.6 |
1811 | 650 | 20.6 | 3250 | - | 192 | 20.6 |
1812 | 650 | 20.6 | 3250 | - | 224 | 20.6 |
1813 | 650 | 20.6 | 3250 | - | 256 | 20.6 |
1814 | 650 | 20.6 | 3250 | - | 288 | 20.6 |
1815 | 650 | 20.6 | 3250 | - | 320 | 20.6 |
1816 | 650 | 20.6 | 3250 | - | 352 | 20.6 |
1609 | 800 | 20.4 | 4000 | - | 40 | 20.4 |
1733 | 750 | 20.3 | 3750 | - | 2405 | 20.3 |
1526 | 900 | 20.1 | 4500 | - | 1125 | 20.1 |
1608 | 800 | 19.8 | 4000 | - | 0 | 19.8 |
1817 | 650 | 19.7 | 3250 | - | 384 | 19.7 |
1818 | 650 | 19.7 | 3250 | - | 416 | 19.7 |
1745 | 700 | 21.2 | 3500 | 0.9 | 385 | 19.5 |
1931 | 600 | 19.2 | 3000 | - | 1410 | 19.2 |
1669 | 750 | 19.1 | 3750 | - | 37 | 19.1 |
1891 | 600 | 19.0 | 3000 | - | 210 | 19.0 |
1892 | 600 | 19.0 | 3000 | - | 240 | 19.0 |
1893 | 600 | 19.0 | 3000 | - | 270 | 19.0 |
1894 | 600 | 19.0 | 3000 | - | 300 | 19.0 |
1895 | 600 | 19.0 | 3000 | - | 330 | 19.0 |
1896 | 600 | 19.0 | 3000 | - | 360 | 19.0 |
1897 | 600 | 19.0 | 3000 | - | 390 | 19.0 |
1898 | 600 | 19.0 | 3000 | - | 420 | 19.0 |
1579 | 850 | 19.0 | 4250 | - | 1134 | 19.0 |
1580 | 850 | 19.0 | 4250 | - | 1176 | 19.0 |
1803 | 700 | 18.9 | 3500 | - | 2415 | 18.9 |
1804 | 700 | 18.9 | 3500 | - | 2450 | 18.9 |
1668 | 750 | 18.6 | 3750 | - | 0 | 18.6 |
1213 | 1300 | 21.6 | 6500 | 1.5 | 780 | 18.5 |
1807 | 650 | 18.3 | 3250 | - | 64 | 18.3 |
1899 | 600 | 18.2 | 3000 | - | 450 | 18.2 |
1979 | 550 | 18.1 | 2750 | - | 243 | 18.1 |
1735 | 700 | 17.9 | 3500 | - | 35 | 17.9 |
1636 | 800 | 17.9 | 4000 | - | 1120 | 17.9 |
1637 | 800 | 17.9 | 4000 | - | 1160 | 17.9 |
1638 | 800 | 17.9 | 4000 | - | 1200 | 17.9 |
1900 | 600 | 18.2 | 3000 | 0.2 | 480 | 17.8 |
1802 | 700 | 18.9 | 3500 | 0.6 | 2380 | 17.8 |
1769 | 700 | 17.8 | 3500 | - | 1225 | 17.8 |
2024 | 550 | 17.6 | 2750 | - | 1458 | 17.6 |
1880 | 650 | 17.6 | 3250 | - | 2400 | 17.6 |
1881 | 650 | 17.6 | 3250 | - | 2432 | 17.6 |
1882 | 650 | 17.6 | 3250 | - | 2464 | 17.6 |
1883 | 650 | 17.6 | 3250 | - | 2496 | 17.6 |
1980 | 550 | 17.4 | 2750 | - | 270 | 17.4 |
1981 | 550 | 17.4 | 2750 | - | 297 | 17.4 |
1982 | 550 | 17.4 | 2750 | - | 324 | 17.4 |
1983 | 550 | 17.4 | 2750 | - | 351 | 17.4 |
1984 | 550 | 17.4 | 2750 | - | 378 | 17.4 |
1985 | 550 | 17.4 | 2750 | - | 405 | 17.4 |
1986 | 550 | 17.4 | 2750 | - | 432 | 17.4 |
1987 | 550 | 17.4 | 2750 | - | 459 | 17.4 |
1734 | 700 | 17.3 | 3500 | - | 0 | 17.3 |
1578 | 850 | 19.0 | 4250 | 0.8 | 1092 | 17.3 |
1701 | 750 | 17.3 | 3750 | - | 1221 | 17.3 |
1886 | 600 | 17.1 | 3000 | - | 60 | 17.1 |
1925 | 600 | 17.1 | 3000 | - | 1230 | 17.1 |
1698 | 750 | 16.7 | 3750 | - | 1110 | 16.7 |
1699 | 750 | 16.7 | 3750 | - | 1147 | 16.7 |
1700 | 750 | 16.7 | 3750 | - | 1184 | 16.7 |
1988 | 550 | 16.7 | 2750 | - | 486 | 16.7 |
1989 | 550 | 16.7 | 2750 | - | 513 | 16.7 |
1431 | 1000 | 16.6 | 5000 | - | 1100 | 16.6 |
1276 | 1200 | 19.9 | 6000 | 1.7 | 900 | 16.6 |
1806 | 650 | 16.6 | 3250 | - | 32 | 16.6 |
2071 | 500 | 16.4 | 2500 | - | 75 | 16.4 |
2016 | 550 | 16.4 | 2750 | - | 1242 | 16.4 |
1964 | 600 | 16.2 | 3000 | - | 2400 | 16.2 |
1965 | 600 | 16.2 | 3000 | - | 2430 | 16.2 |
1966 | 600 | 16.2 | 3000 | - | 2460 | 16.2 |
1967 | 600 | 16.2 | 3000 | - | 2490 | 16.2 |
1968 | 600 | 16.2 | 3000 | - | 2520 | 16.2 |
1969 | 600 | 16.2 | 3000 | - | 2550 | 16.2 |
1805 | 650 | 16.1 | 3250 | - | 0 | 16.1 |
1732 | 750 | 20.3 | 3750 | 2.1 | 2368 | 16.0 |
2080 | 500 | 15.9 | 2500 | - | 300 | 15.9 |
2081 | 500 | 15.8 | 2500 | - | 325 | 15.8 |
2082 | 500 | 15.8 | 2500 | - | 350 | 15.8 |
2083 | 500 | 15.8 | 2500 | - | 375 | 15.8 |
2084 | 500 | 15.8 | 2500 | - | 400 | 15.8 |
2085 | 500 | 15.8 | 2500 | - | 425 | 15.8 |
2086 | 500 | 15.8 | 2500 | - | 450 | 15.8 |
2087 | 500 | 15.8 | 2500 | - | 475 | 15.8 |
2088 | 500 | 15.8 | 2500 | - | 500 | 15.8 |
1477 | 950 | 15.8 | 4750 | - | 1128 | 15.8 |
2089 | 500 | 15.7 | 2500 | - | 525 | 15.7 |
1766 | 700 | 15.6 | 3500 | - | 1120 | 15.6 |
1767 | 700 | 15.6 | 3500 | - | 1155 | 15.6 |
1768 | 700 | 15.6 | 3500 | - | 1190 | 15.6 |
1885 | 600 | 15.3 | 3000 | - | 30 | 15.3 |
2090 | 500 | 15.2 | 2500 | - | 550 | 15.2 |
2091 | 500 | 15.2 | 2500 | - | 575 | 15.2 |
1639 | 800 | 15.0 | 4000 | - | 1240 | 15.0 |
1527 | 900 | 15.0 | 4500 | - | 1170 | 15.0 |
1972 | 550 | 14.9 | 2750 | - | 54 | 14.9 |
2059 | 550 | 14.9 | 2750 | - | 2403 | 14.9 |
2060 | 550 | 14.9 | 2750 | - | 2430 | 14.9 |
2061 | 550 | 14.9 | 2750 | - | 2457 | 14.9 |
2062 | 550 | 14.9 | 2750 | - | 2484 | 14.9 |
2063 | 550 | 14.9 | 2750 | - | 2511 | 14.9 |
2064 | 550 | 14.9 | 2750 | - | 2538 | 14.9 |
2065 | 550 | 14.9 | 2750 | - | 2565 | 14.9 |
2066 | 550 | 14.9 | 2750 | - | 2592 | 14.9 |
2067 | 550 | 14.9 | 2750 | - | 2619 | 14.9 |
1884 | 600 | 14.9 | 3000 | - | 0 | 14.9 |
1848 | 650 | 14.8 | 3250 | - | 1376 | 14.8 |
1244 | 1250 | 20.8 | 6250 | 3.0 | 868 | 14.7 |
1772 | 700 | 14.6 | 3500 | - | 1330 | 14.6 |
1348 | 1100 | 18.3 | 5500 | 1.8 | 990 | 14.6 |
1840 | 650 | 14.5 | 3250 | - | 1120 | 14.5 |
1841 | 650 | 14.5 | 3250 | - | 1152 | 14.5 |
1842 | 650 | 14.5 | 3250 | - | 1184 | 14.5 |
1843 | 650 | 14.5 | 3250 | - | 1216 | 14.5 |
1581 | 850 | 14.1 | 4250 | - | 1218 | 14.1 |
1971 | 550 | 14.0 | 2750 | - | 27 | 14.0 |
1388 | 1050 | 17.4 | 5250 | 1.9 | 1040 | 13.6 |
1970 | 550 | 13.6 | 2750 | - | 0 | 13.6 |
2173 | 500 | 13.6 | 2500 | - | 2625 | 13.6 |
2164 | 500 | 13.5 | 2500 | - | 2400 | 13.5 |
2165 | 500 | 13.5 | 2500 | - | 2425 | 13.5 |
2166 | 500 | 13.5 | 2500 | - | 2450 | 13.5 |
2167 | 500 | 13.5 | 2500 | - | 2475 | 13.5 |
2168 | 500 | 13.5 | 2500 | - | 2500 | 13.5 |
2169 | 500 | 13.5 | 2500 | - | 2525 | 13.5 |
2170 | 500 | 13.5 | 2500 | - | 2550 | 13.5 |
2171 | 500 | 13.5 | 2500 | - | 2575 | 13.5 |
2172 | 500 | 13.5 | 2500 | - | 2600 | 13.5 |
1921 | 600 | 13.4 | 3000 | - | 1110 | 13.4 |
1922 | 600 | 13.4 | 3000 | - | 1140 | 13.4 |
1923 | 600 | 13.4 | 3000 | - | 1170 | 13.4 |
1924 | 600 | 13.4 | 3000 | - | 1200 | 13.4 |
1640 | 800 | 13.3 | 4000 | - | 1280 | 13.3 |
2070 | 500 | 13.2 | 2500 | - | 50 | 13.2 |
1703 | 750 | 13.0 | 3750 | - | 1295 | 13.0 |
1667 | 800 | 18.8 | 4000 | 3.0 | 2360 | 12.8 |
2069 | 500 | 12.8 | 2500 | - | 25 | 12.8 |
2117 | 500 | 12.7 | 2500 | - | 1225 | 12.7 |
1879 | 650 | 17.6 | 3250 | 2.5 | 2368 | 12.6 |
1704 | 750 | 12.5 | 3750 | - | 1332 | 12.5 |
2068 | 500 | 12.4 | 2500 | - | 0 | 12.4 |
1311 | 1150 | 19.1 | 5750 | 3.4 | 969 | 12.3 |
2011 | 550 | 12.3 | 2750 | - | 1107 | 12.3 |
2012 | 550 | 12.3 | 2750 | - | 1134 | 12.3 |
2013 | 550 | 12.3 | 2750 | - | 1161 | 12.3 |
2014 | 550 | 12.3 | 2750 | - | 1188 | 12.3 |
2015 | 550 | 12.3 | 2750 | - | 1215 | 12.3 |
2058 | 550 | 14.9 | 2750 | 1.5 | 2376 | 12.0 |
1582 | 850 | 14.1 | 4250 | 1.2 | 1260 | 11.8 |
2129 | 500 | 11.7 | 2500 | - | 1525 | 11.7 |
1773 | 700 | 11.6 | 3500 | - | 1365 | 11.6 |
1774 | 700 | 11.6 | 3500 | - | 1400 | 11.6 |
1528 | 900 | 15.0 | 4500 | 1.7 | 1215 | 11.6 |
1214 | 1300 | 21.6 | 6500 | 5.0 | 845 | 11.6 |
1963 | 600 | 16.2 | 3000 | 2.3 | 2370 | 11.5 |
1932 | 600 | 11.3 | 3000 | - | 1440 | 11.3 |
2112 | 500 | 11.2 | 2500 | - | 1100 | 11.2 |
2113 | 500 | 11.2 | 2500 | - | 1125 | 11.2 |
2114 | 500 | 11.2 | 2500 | - | 1150 | 11.2 |
2115 | 500 | 11.2 | 2500 | - | 1175 | 11.2 |
2116 | 500 | 11.2 | 2500 | - | 1200 | 11.2 |
1849 | 650 | 10.8 | 3250 | - | 1408 | 10.8 |
1850 | 650 | 10.8 | 3250 | - | 1440 | 10.8 |
2025 | 550 | 10.6 | 2750 | - | 1485 | 10.6 |
1478 | 950 | 15.8 | 4750 | 2.6 | 1175 | 10.5 |
2163 | 500 | 13.5 | 2500 | 1.8 | 2375 | 9.9 |
1641 | 800 | 13.3 | 4000 | 2.5 | 1320 | 8.3 |
1389 | 1050 | 17.4 | 5250 | 4.7 | 1092 | 8.1 |
1705 | 750 | 12.5 | 3750 | 2.5 | 1369 | 7.4 |
1432 | 1000 | 16.6 | 5000 | 5.0 | 1150 | 6.6 |
1851 | 650 | 10.8 | 3250 | 3.4 | 1472 | 4.0 |
1775 | 700 | 11.6 | 3500 | 5.0 | 1435 | 1.6 |
Overall score = score_ratio - 2 * repetitive DNA content
Redundant probes
Putative probe ID | Length | Score ratio | Actual | %rep DNA | Distance | Overall |
---|---|---|---|---|---|---|
1262 | 1200 | 31.9 | 6000 | 1.7 | 60 | 28.6 |
1230 | 1250 | 30.9 | 6250 | 1.6 | 0 | 27.7 |
1295 | 1150 | 30.6 | 5750 | 1.7 | 57 | 27.1 |
1296 | 1150 | 30.6 | 5750 | 1.7 | 114 | 27.1 |
1261 | 1200 | 29.7 | 6000 | 1.7 | 0 | 26.4 |
1411 | 1000 | 30.3 | 5000 | 2.0 | 100 | 26.3 |
1331 | 1100 | 29.3 | 5500 | 1.8 | 55 | 25.6 |
1332 | 1100 | 29.3 | 5500 | 1.8 | 110 | 25.6 |
1333 | 1100 | 29.3 | 5500 | 1.8 | 165 | 25.6 |
1294 | 1150 | 28.5 | 5750 | 1.7 | 0 | 25.0 |
1235 | 1250 | 27.9 | 6250 | 1.6 | 310 | 24.7 |
1236 | 1250 | 27.9 | 6250 | 1.6 | 372 | 24.7 |
1237 | 1250 | 27.9 | 6250 | 1.6 | 434 | 24.7 |
1238 | 1250 | 27.9 | 6250 | 1.6 | 496 | 24.7 |
1239 | 1250 | 27.9 | 6250 | 1.6 | 558 | 24.7 |
1240 | 1250 | 27.9 | 6250 | 1.6 | 620 | 24.7 |
1241 | 1250 | 27.9 | 6250 | 1.6 | 682 | 24.7 |
1242 | 1250 | 27.9 | 6250 | 1.6 | 744 | 24.7 |
1370 | 1050 | 27.9 | 5250 | 1.9 | 104 | 24.1 |
1371 | 1050 | 27.9 | 5250 | 1.9 | 156 | 24.1 |
1372 | 1050 | 27.9 | 5250 | 1.9 | 208 | 24.1 |
1369 | 1050 | 27.6 | 5250 | 1.9 | 52 | 23.8 |
1330 | 1100 | 27.2 | 5500 | 1.8 | 0 | 23.6 |
1263 | 1200 | 26.8 | 6000 | 1.7 | 120 | 23.5 |
1264 | 1200 | 26.8 | 6000 | 1.7 | 180 | 23.5 |
1265 | 1200 | 26.8 | 6000 | 1.7 | 240 | 23.5 |
1266 | 1200 | 26.8 | 6000 | 1.7 | 300 | 23.5 |
1267 | 1200 | 26.8 | 6000 | 1.7 | 360 | 23.5 |
1268 | 1200 | 26.8 | 6000 | 1.7 | 420 | 23.5 |
1269 | 1200 | 26.8 | 6000 | 1.7 | 480 | 23.5 |
1270 | 1200 | 26.8 | 6000 | 1.7 | 540 | 23.5 |
1271 | 1200 | 26.8 | 6000 | 1.7 | 600 | 23.5 |
1272 | 1200 | 26.8 | 6000 | 1.7 | 660 | 23.5 |
1273 | 1200 | 26.8 | 6000 | 1.7 | 720 | 23.5 |
1274 | 1200 | 26.8 | 6000 | 1.7 | 780 | 23.5 |
1412 | 1000 | 26.6 | 5000 | 2.0 | 150 | 22.6 |
1413 | 1000 | 26.6 | 5000 | 2.0 | 200 | 22.6 |
1414 | 1000 | 26.6 | 5000 | 2.0 | 250 | 22.6 |
1297 | 1150 | 25.7 | 5750 | 1.7 | 171 | 22.2 |
1298 | 1150 | 25.7 | 5750 | 1.7 | 228 | 22.2 |
1299 | 1150 | 25.7 | 5750 | 1.7 | 285 | 22.2 |
1300 | 1150 | 25.7 | 5750 | 1.7 | 342 | 22.2 |
1301 | 1150 | 25.7 | 5750 | 1.7 | 399 | 22.2 |
1302 | 1150 | 25.7 | 5750 | 1.7 | 456 | 22.2 |
1303 | 1150 | 25.7 | 5750 | 1.7 | 513 | 22.2 |
1304 | 1150 | 25.7 | 5750 | 1.7 | 570 | 22.2 |
1305 | 1150 | 25.7 | 5750 | 1.7 | 627 | 22.2 |
1306 | 1150 | 25.7 | 5750 | 1.7 | 684 | 22.2 |
1307 | 1150 | 25.7 | 5750 | 1.7 | 741 | 22.2 |
1308 | 1150 | 25.7 | 5750 | 1.7 | 798 | 22.2 |
1309 | 1150 | 25.7 | 5750 | 1.7 | 855 | 22.2 |
1457 | 950 | 26.1 | 4750 | 2.1 | 188 | 21.9 |
1506 | 900 | 25.6 | 4500 | 2.2 | 225 | 21.1 |
1458 | 950 | 25.3 | 4750 | 2.1 | 235 | 21.1 |
1459 | 950 | 25.3 | 4750 | 2.1 | 282 | 21.1 |
1558 | 850 | 25.8 | 4250 | 2.4 | 252 | 21.1 |
1334 | 1100 | 24.6 | 5500 | 1.8 | 220 | 20.9 |
1335 | 1100 | 24.6 | 5500 | 1.8 | 275 | 20.9 |
1336 | 1100 | 24.6 | 5500 | 1.8 | 330 | 20.9 |
1337 | 1100 | 24.6 | 5500 | 1.8 | 385 | 20.9 |
1338 | 1100 | 24.6 | 5500 | 1.8 | 440 | 20.9 |
1339 | 1100 | 24.6 | 5500 | 1.8 | 495 | 20.9 |
1340 | 1100 | 24.6 | 5500 | 1.8 | 550 | 20.9 |
1341 | 1100 | 24.6 | 5500 | 1.8 | 605 | 20.9 |
1342 | 1100 | 24.6 | 5500 | 1.8 | 660 | 20.9 |
1343 | 1100 | 24.6 | 5500 | 1.8 | 715 | 20.9 |
1344 | 1100 | 24.6 | 5500 | 1.8 | 770 | 20.9 |
1345 | 1100 | 24.6 | 5500 | 1.8 | 825 | 20.9 |
1346 | 1100 | 24.6 | 5500 | 1.8 | 880 | 20.9 |
1373 | 1050 | 23.4 | 5250 | 1.9 | 260 | 19.6 |
1374 | 1050 | 23.4 | 5250 | 1.9 | 312 | 19.6 |
1375 | 1050 | 23.4 | 5250 | 1.9 | 364 | 19.6 |
1376 | 1050 | 23.4 | 5250 | 1.9 | 416 | 19.6 |
1377 | 1050 | 23.4 | 5250 | 1.9 | 468 | 19.6 |
1378 | 1050 | 23.4 | 5250 | 1.9 | 520 | 19.6 |
1379 | 1050 | 23.4 | 5250 | 1.9 | 572 | 19.6 |
1380 | 1050 | 23.4 | 5250 | 1.9 | 624 | 19.6 |
1381 | 1050 | 23.4 | 5250 | 1.9 | 676 | 19.6 |
1382 | 1050 | 23.4 | 5250 | 1.9 | 728 | 19.6 |
1383 | 1050 | 23.4 | 5250 | 1.9 | 780 | 19.6 |
1384 | 1050 | 23.4 | 5250 | 1.9 | 832 | 19.6 |
1385 | 1050 | 23.4 | 5250 | 1.9 | 884 | 19.6 |
1386 | 1050 | 23.4 | 5250 | 1.9 | 936 | 19.6 |
1507 | 900 | 23.9 | 4500 | 2.2 | 270 | 19.5 |
1508 | 900 | 23.9 | 4500 | 2.2 | 315 | 19.5 |
1509 | 900 | 23.9 | 4500 | 2.2 | 360 | 19.5 |
1275 | 1200 | 22.6 | 6000 | 1.7 | 840 | 19.2 |
1616 | 800 | 23.4 | 4000 | 2.5 | 320 | 18.4 |
1415 | 1000 | 22.3 | 5000 | 2.0 | 300 | 18.3 |
1416 | 1000 | 22.3 | 5000 | 2.0 | 350 | 18.3 |
1417 | 1000 | 22.3 | 5000 | 2.0 | 400 | 18.3 |
1418 | 1000 | 22.3 | 5000 | 2.0 | 450 | 18.3 |
1419 | 1000 | 22.3 | 5000 | 2.0 | 500 | 18.3 |
1420 | 1000 | 22.3 | 5000 | 2.0 | 550 | 18.3 |
1421 | 1000 | 22.3 | 5000 | 2.0 | 600 | 18.3 |
1422 | 1000 | 22.3 | 5000 | 2.0 | 650 | 18.3 |
1423 | 1000 | 22.3 | 5000 | 2.0 | 700 | 18.3 |
1424 | 1000 | 22.3 | 5000 | 2.0 | 750 | 18.3 |
1425 | 1000 | 22.3 | 5000 | 2.0 | 800 | 18.3 |
1426 | 1000 | 22.3 | 5000 | 2.0 | 850 | 18.3 |
1427 | 1000 | 22.3 | 5000 | 2.0 | 900 | 18.3 |
1428 | 1000 | 22.3 | 5000 | 2.0 | 950 | 18.3 |
1429 | 1000 | 22.3 | 5000 | 2.0 | 1000 | 18.3 |
1559 | 850 | 22.6 | 4250 | 2.4 | 294 | 17.9 |
1560 | 850 | 22.6 | 4250 | 2.4 | 336 | 17.9 |
1561 | 850 | 22.6 | 4250 | 2.4 | 378 | 17.9 |
1347 | 1100 | 21.2 | 5500 | 1.8 | 935 | 17.6 |
1460 | 950 | 21.8 | 4750 | 2.1 | 329 | 17.6 |
1243 | 1250 | 20.8 | 6250 | 1.6 | 806 | 17.6 |
1562 | 850 | 21.8 | 4250 | 2.4 | 420 | 17.1 |
1461 | 950 | 21.2 | 4750 | 2.1 | 376 | 17.0 |
1462 | 950 | 21.2 | 4750 | 2.1 | 423 | 17.0 |
1463 | 950 | 21.2 | 4750 | 2.1 | 470 | 17.0 |
1464 | 950 | 21.2 | 4750 | 2.1 | 517 | 17.0 |
1465 | 950 | 21.2 | 4750 | 2.1 | 564 | 17.0 |
1466 | 950 | 21.2 | 4750 | 2.1 | 611 | 17.0 |
1467 | 950 | 21.2 | 4750 | 2.1 | 658 | 17.0 |
1468 | 950 | 21.2 | 4750 | 2.1 | 705 | 17.0 |
1469 | 950 | 21.2 | 4750 | 2.1 | 752 | 17.0 |
1470 | 950 | 21.2 | 4750 | 2.1 | 799 | 17.0 |
1471 | 950 | 21.2 | 4750 | 2.1 | 846 | 17.0 |
1472 | 950 | 21.2 | 4750 | 2.1 | 893 | 17.0 |
1473 | 950 | 21.2 | 4750 | 2.1 | 940 | 17.0 |
1474 | 950 | 21.2 | 4750 | 2.1 | 987 | 17.0 |
1475 | 950 | 21.2 | 4750 | 2.1 | 1034 | 17.0 |
1678 | 750 | 21.9 | 3750 | 2.7 | 370 | 16.6 |
1617 | 800 | 21.3 | 4000 | 2.5 | 360 | 16.3 |
1618 | 800 | 21.3 | 4000 | 2.5 | 400 | 16.3 |
1619 | 800 | 21.3 | 4000 | 2.5 | 440 | 16.3 |
1387 | 1050 | 19.8 | 5250 | 1.9 | 988 | 16.0 |
1525 | 900 | 20.1 | 4500 | 2.1 | 1080 | 15.9 |
1476 | 950 | 19.5 | 4750 | 1.9 | 1081 | 15.7 |
1510 | 900 | 20.1 | 4500 | 2.2 | 405 | 15.6 |
1511 | 900 | 20.1 | 4500 | 2.2 | 450 | 15.6 |
1512 | 900 | 20.1 | 4500 | 2.2 | 495 | 15.6 |
1513 | 900 | 20.1 | 4500 | 2.2 | 540 | 15.6 |
1514 | 900 | 20.1 | 4500 | 2.2 | 585 | 15.6 |
1515 | 900 | 20.1 | 4500 | 2.2 | 630 | 15.6 |
1516 | 900 | 20.1 | 4500 | 2.2 | 675 | 15.6 |
1517 | 900 | 20.1 | 4500 | 2.2 | 720 | 15.6 |
1518 | 900 | 20.1 | 4500 | 2.2 | 765 | 15.6 |
1519 | 900 | 20.1 | 4500 | 2.2 | 810 | 15.6 |
1520 | 900 | 20.1 | 4500 | 2.2 | 855 | 15.6 |
1521 | 900 | 20.1 | 4500 | 2.2 | 900 | 15.6 |
1522 | 900 | 20.1 | 4500 | 2.2 | 945 | 15.6 |
1523 | 900 | 20.1 | 4500 | 2.2 | 990 | 15.6 |
1524 | 900 | 20.1 | 4500 | 2.2 | 1035 | 15.6 |
1310 | 1150 | 19.1 | 5750 | 1.7 | 912 | 15.6 |
1746 | 700 | 20.5 | 3500 | 2.9 | 420 | 14.8 |
1679 | 750 | 19.9 | 3750 | 2.7 | 407 | 14.6 |
1680 | 750 | 19.9 | 3750 | 2.7 | 444 | 14.6 |
1681 | 750 | 19.9 | 3750 | 2.7 | 481 | 14.6 |
1682 | 750 | 19.7 | 3750 | 2.7 | 518 | 14.4 |
1563 | 850 | 19.0 | 4250 | 2.4 | 462 | 14.3 |
1564 | 850 | 19.0 | 4250 | 2.4 | 504 | 14.3 |
1565 | 850 | 19.0 | 4250 | 2.4 | 546 | 14.3 |
1566 | 850 | 19.0 | 4250 | 2.4 | 588 | 14.3 |
1567 | 850 | 19.0 | 4250 | 2.4 | 630 | 14.3 |
1568 | 850 | 19.0 | 4250 | 2.4 | 672 | 14.3 |
1569 | 850 | 19.0 | 4250 | 2.4 | 714 | 14.3 |
1570 | 850 | 19.0 | 4250 | 2.4 | 756 | 14.3 |
1571 | 850 | 19.0 | 4250 | 2.4 | 798 | 14.3 |
1572 | 850 | 19.0 | 4250 | 2.4 | 840 | 14.3 |
1573 | 850 | 19.0 | 4250 | 2.4 | 882 | 14.3 |
1574 | 850 | 19.0 | 4250 | 2.4 | 924 | 14.3 |
1575 | 850 | 19.0 | 4250 | 2.4 | 966 | 14.3 |
1576 | 850 | 19.0 | 4250 | 2.4 | 1008 | 14.3 |
1577 | 850 | 19.0 | 4250 | 2.4 | 1050 | 14.3 |
1819 | 650 | 19.7 | 3250 | 2.9 | 448 | 13.9 |
1620 | 800 | 18.3 | 4000 | 2.5 | 480 | 13.3 |
1635 | 800 | 17.9 | 4000 | 2.4 | 1080 | 13.1 |
1747 | 700 | 18.6 | 3500 | 2.9 | 455 | 12.9 |
1748 | 700 | 18.6 | 3500 | 2.9 | 490 | 12.9 |
1749 | 700 | 18.6 | 3500 | 2.9 | 525 | 12.9 |
1750 | 700 | 18.6 | 3500 | 2.9 | 560 | 12.9 |
1621 | 800 | 17.9 | 4000 | 2.5 | 520 | 12.9 |
1622 | 800 | 17.9 | 4000 | 2.5 | 560 | 12.9 |
1623 | 800 | 17.9 | 4000 | 2.5 | 600 | 12.9 |
1624 | 800 | 17.9 | 4000 | 2.5 | 640 | 12.9 |
1625 | 800 | 17.9 | 4000 | 2.5 | 680 | 12.9 |
1626 | 800 | 17.9 | 4000 | 2.5 | 720 | 12.9 |
1627 | 800 | 17.9 | 4000 | 2.5 | 760 | 12.9 |
1628 | 800 | 17.9 | 4000 | 2.5 | 800 | 12.9 |
1629 | 800 | 17.9 | 4000 | 2.5 | 840 | 12.9 |
1630 | 800 | 17.9 | 4000 | 2.5 | 880 | 12.9 |
1631 | 800 | 17.9 | 4000 | 2.5 | 920 | 12.9 |
1632 | 800 | 17.9 | 4000 | 2.5 | 960 | 12.9 |
1633 | 800 | 17.9 | 4000 | 2.5 | 1000 | 12.9 |
1634 | 800 | 17.9 | 4000 | 2.5 | 1040 | 12.9 |
1430 | 1000 | 16.7 | 5000 | 2.0 | 1050 | 12.7 |
1990 | 550 | 16.7 | 2750 | 2.0 | 540 | 12.7 |
1765 | 700 | 15.6 | 3500 | 2.0 | 1085 | 11.6 |
1820 | 650 | 17.6 | 3250 | 3.1 | 480 | 11.4 |
1683 | 750 | 16.7 | 3750 | 2.7 | 555 | 11.4 |
1684 | 750 | 16.7 | 3750 | 2.7 | 592 | 11.4 |
1685 | 750 | 16.7 | 3750 | 2.7 | 629 | 11.4 |
1686 | 750 | 16.7 | 3750 | 2.7 | 666 | 11.4 |
1687 | 750 | 16.7 | 3750 | 2.7 | 703 | 11.4 |
1688 | 750 | 16.7 | 3750 | 2.7 | 740 | 11.4 |
1689 | 750 | 16.7 | 3750 | 2.7 | 777 | 11.4 |
1690 | 750 | 16.7 | 3750 | 2.7 | 814 | 11.4 |
1691 | 750 | 16.7 | 3750 | 2.7 | 851 | 11.4 |
1692 | 750 | 16.7 | 3750 | 2.7 | 888 | 11.4 |
1693 | 750 | 16.7 | 3750 | 2.7 | 925 | 11.4 |
1694 | 750 | 16.7 | 3750 | 2.7 | 962 | 11.4 |
1695 | 750 | 16.7 | 3750 | 2.7 | 999 | 11.4 |
1696 | 750 | 16.7 | 3750 | 2.7 | 1036 | 11.4 |
1697 | 750 | 16.7 | 3750 | 2.7 | 1073 | 11.4 |
1821 | 650 | 17.3 | 3250 | 3.1 | 512 | 11.1 |
1822 | 650 | 17.3 | 3250 | 3.1 | 544 | 11.1 |
1823 | 650 | 17.3 | 3250 | 3.1 | 576 | 11.1 |
1824 | 650 | 17.3 | 3250 | 3.1 | 608 | 11.1 |
1839 | 650 | 14.5 | 3250 | 1.7 | 1088 | 11.1 |
1901 | 600 | 17.6 | 3000 | 3.3 | 510 | 11.0 |
1751 | 700 | 15.6 | 3500 | 2.9 | 595 | 9.9 |
1752 | 700 | 15.6 | 3500 | 2.9 | 630 | 9.9 |
1753 | 700 | 15.6 | 3500 | 2.9 | 665 | 9.9 |
1754 | 700 | 15.6 | 3500 | 2.9 | 700 | 9.9 |
1755 | 700 | 15.6 | 3500 | 2.9 | 735 | 9.9 |
1756 | 700 | 15.6 | 3500 | 2.9 | 770 | 9.9 |
1757 | 700 | 15.6 | 3500 | 2.9 | 805 | 9.9 |
1758 | 700 | 15.6 | 3500 | 2.9 | 840 | 9.9 |
1759 | 700 | 15.6 | 3500 | 2.9 | 875 | 9.9 |
1760 | 700 | 15.6 | 3500 | 2.9 | 910 | 9.9 |
1761 | 700 | 15.6 | 3500 | 2.9 | 945 | 9.9 |
1762 | 700 | 15.6 | 3500 | 2.9 | 980 | 9.9 |
1763 | 700 | 15.6 | 3500 | 2.9 | 1015 | 9.9 |
1764 | 700 | 15.6 | 3500 | 2.9 | 1050 | 9.9 |
1902 | 600 | 16.5 | 3000 | 3.3 | 540 | 9.8 |
1903 | 600 | 16.0 | 3000 | 3.3 | 570 | 9.3 |
1904 | 600 | 16.0 | 3000 | 3.3 | 600 | 9.3 |
1905 | 600 | 16.0 | 3000 | 3.3 | 630 | 9.3 |
1906 | 600 | 16.0 | 3000 | 3.3 | 660 | 9.3 |
1991 | 550 | 16.1 | 2750 | 3.6 | 567 | 8.8 |
1825 | 650 | 14.5 | 3250 | 3.1 | 640 | 8.4 |
1826 | 650 | 14.5 | 3250 | 3.1 | 672 | 8.4 |
1827 | 650 | 14.5 | 3250 | 3.1 | 704 | 8.4 |
1828 | 650 | 14.5 | 3250 | 3.1 | 736 | 8.4 |
1829 | 650 | 14.5 | 3250 | 3.1 | 768 | 8.4 |
1830 | 650 | 14.5 | 3250 | 3.1 | 800 | 8.4 |
1831 | 650 | 14.5 | 3250 | 3.1 | 832 | 8.4 |
1832 | 650 | 14.5 | 3250 | 3.1 | 864 | 8.4 |
1833 | 650 | 14.5 | 3250 | 3.1 | 896 | 8.4 |
1834 | 650 | 14.5 | 3250 | 3.1 | 928 | 8.4 |
1835 | 650 | 14.5 | 3250 | 3.1 | 960 | 8.4 |
1836 | 650 | 14.5 | 3250 | 3.1 | 992 | 8.4 |
1837 | 650 | 14.5 | 3250 | 3.1 | 1024 | 8.4 |
1838 | 650 | 14.5 | 3250 | 3.1 | 1056 | 8.4 |
1992 | 550 | 14.6 | 2750 | 3.6 | 594 | 7.4 |
1993 | 550 | 14.6 | 2750 | 3.6 | 621 | 7.4 |
1994 | 550 | 14.6 | 2750 | 3.6 | 648 | 7.4 |
1995 | 550 | 14.6 | 2750 | 3.6 | 675 | 7.4 |
1996 | 550 | 14.6 | 2750 | 3.6 | 702 | 7.4 |
2092 | 500 | 15.2 | 2500 | 4.0 | 600 | 7.2 |
1920 | 600 | 13.4 | 3000 | 3.2 | 1080 | 7.1 |
1907 | 600 | 13.4 | 3000 | 3.3 | 690 | 6.7 |
1908 | 600 | 13.4 | 3000 | 3.3 | 720 | 6.7 |
1909 | 600 | 13.4 | 3000 | 3.3 | 750 | 6.7 |
1910 | 600 | 13.4 | 3000 | 3.3 | 780 | 6.7 |
1911 | 600 | 13.4 | 3000 | 3.3 | 810 | 6.7 |
1912 | 600 | 13.4 | 3000 | 3.3 | 840 | 6.7 |
1913 | 600 | 13.4 | 3000 | 3.3 | 870 | 6.7 |
1914 | 600 | 13.4 | 3000 | 3.3 | 900 | 6.7 |
1915 | 600 | 13.4 | 3000 | 3.3 | 930 | 6.7 |
1916 | 600 | 13.4 | 3000 | 3.3 | 960 | 6.7 |
1917 | 600 | 13.4 | 3000 | 3.3 | 990 | 6.7 |
1918 | 600 | 13.4 | 3000 | 3.3 | 1020 | 6.7 |
1919 | 600 | 13.4 | 3000 | 3.3 | 1050 | 6.7 |
2093 | 500 | 14.2 | 2500 | 4.0 | 625 | 6.2 |
2010 | 550 | 12.3 | 2750 | 3.5 | 1080 | 5.4 |
1997 | 550 | 12.6 | 2750 | 3.6 | 729 | 5.3 |
2094 | 500 | 13.3 | 2500 | 4.0 | 650 | 5.3 |
2095 | 500 | 13.3 | 2500 | 4.0 | 675 | 5.3 |
2096 | 500 | 13.3 | 2500 | 4.0 | 700 | 5.3 |
2097 | 500 | 13.3 | 2500 | 4.0 | 725 | 5.3 |
2098 | 500 | 13.3 | 2500 | 4.0 | 750 | 5.3 |
1998 | 550 | 12.3 | 2750 | 3.6 | 756 | 5.0 |
1999 | 550 | 12.3 | 2750 | 3.6 | 783 | 5.0 |
2000 | 550 | 12.3 | 2750 | 3.6 | 810 | 5.0 |
2001 | 550 | 12.3 | 2750 | 3.6 | 837 | 5.0 |
2002 | 550 | 12.3 | 2750 | 3.6 | 864 | 5.0 |
2003 | 550 | 12.3 | 2750 | 3.6 | 891 | 5.0 |
2004 | 550 | 12.3 | 2750 | 3.6 | 918 | 5.0 |
2005 | 550 | 12.3 | 2750 | 3.6 | 945 | 5.0 |
2006 | 550 | 12.3 | 2750 | 3.6 | 972 | 5.0 |
2007 | 550 | 12.3 | 2750 | 3.6 | 999 | 5.0 |
2008 | 550 | 12.3 | 2750 | 3.6 | 1026 | 5.0 |
2009 | 550 | 12.3 | 2750 | 3.6 | 1053 | 5.0 |
2099 | 500 | 12.4 | 2500 | 4.0 | 775 | 4.4 |
2100 | 500 | 11.2 | 2500 | 4.0 | 800 | 3.2 |
2101 | 500 | 11.2 | 2500 | 4.0 | 825 | 3.2 |
2102 | 500 | 11.2 | 2500 | 4.0 | 850 | 3.2 |
2103 | 500 | 11.2 | 2500 | 4.0 | 875 | 3.2 |
2104 | 500 | 11.2 | 2500 | 4.0 | 900 | 3.2 |
2105 | 500 | 11.2 | 2500 | 4.0 | 925 | 3.2 |
2106 | 500 | 11.2 | 2500 | 4.0 | 950 | 3.2 |
2107 | 500 | 11.2 | 2500 | 4.0 | 975 | 3.2 |
2108 | 500 | 11.2 | 2500 | 4.0 | 1000 | 3.2 |
2109 | 500 | 11.2 | 2500 | 4.0 | 1025 | 3.2 |
2110 | 500 | 11.2 | 2500 | 4.0 | 1050 | 3.2 |
2111 | 500 | 11.2 | 2500 | 4.0 | 1075 | 3.2 |
Overall score = score_ratio - 2 * repetitive DNA content