Summary

./analyse_probe_search_results : Fri Feb 17 10:01:30 2006

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign115
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 15
  id            : 111
  strand        : 1
  -------------

Design window length: 4501bp

All jobs for probe design are successful - total: 77
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 1526 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1526/1526
Putative probes that are unique                 : 108/1526
Putative probes exceeding selection criteria    : 130/1526 (both unique and not unique)
Putative probes with successfully-picked primers: 130/130

Identified 0 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probes

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

No probes

Overall score = score_ratio - 2 * repetitive DNA content