G2Cdb::Allele report

Mutation type
TF

Altered genes (1)

Gene Symbol Species Description
G00002371 CALM1 Homo sapiens calmodulin 1 (phosphorylase kinase, delta)

Diseases (1)

Disease Description Nervous effect
D00000103 Leukaemia N

Literature (1)

Pubmed - human_disease

  • Leukaemic transformation by CALM-AF10 involves upregulation of Hoxa5 by hDOT1L.

    Okada Y, Jiang Q, Lemieux M, Jeannotte L, Su L and Zhang Y

    Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA.

    Chromosomal translocation is a common cause of leukaemia and the most common chromosome translocations found in leukaemia patients involve the mixed lineage leukaemia (MLL) gene. AF10 is one of more than 30 MLL fusion partners in leukaemia. We have recently demonstrated that the H3K79 methyltransferase hDOT1L contributes to MLL-AF10-mediated leukaemogenesis through its interaction with AF10 (ref. 5). In addition to MLL, AF10 has also been reported to fuse to CALM (clathrin-assembly protein-like lymphoid-myeloid) in patients with T-cell acute lymphoblastic leukaemia (T-ALL) and acute myeloid leukaemia (AML). Here, we analysed the molecular mechanism of leukaemogenesis by CALM-AF10. We demonstrate that CALM-AF10 fusion is both necessary and sufficient for leukaemic transformation. Additionally, we provide evidence that hDOT1L has an important role in the transformation process. hDOT1L contributes to CALM-AF10-mediated leukaemic transformation by preventing nuclear export of CALM-AF10 and by upregulating the Hoxa5 gene through H3K79 methylation. Thus, our study establishes CALM-AF10 fusion as a cause of leukaemia and reveals that mistargeting of hDOT1L and upregulation of Hoxa5 through H3K79 methylation is the underlying mechanism behind leukaemia caused by CALM-AF10 fusion.

    Funded by: NHLBI NIH HHS: HL72240, R21 HL072240; NIAID NIH HHS: AI48407, R01 AI048407, R01 AI077454, R01 AI080432, R56 AI048407; NIGMS NIH HHS: GM68804, R01 GM068804

    Nature cell biology 2006;8;9;1017-24

© G2C 2014. The Genes to Cognition Programme received funding from The Wellcome Trust and the EU FP7 Framework Programmes:
EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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