G2C::Proteomics

Targeted tandem affinity purification of PSD-95 recovers core
postsynaptic complexes and schizophrenia susceptibility proteins
Esperanza Fernandez, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama,
Mike DR Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary and Seth GN Grant


Table 2. Functional classification of PSD-95-associated proteins in one or two tandem purifications.

Approved gene symbols and protein names, and UniProt accession numbers are shown. Number of approved peptides for each protein identified by LC-MS/MS in the four tandem purifications are indicated as T1, T2, T3 and T4. More information of these proteins is listed in Supplementary Table 1.

MGI SymbolApproved NameUniProt Acc
Number of peptides
Adaptor/ RegulatoryT1T2T3T4
Ap2a1adaptor protein complex AP-2, alpha 1 subunitP174263
Grb2growth factor receptor bound protein 2Q6063124
Receptors/ Channels/ Transporters
Atp2b1ATPase, Ca++ transporting, plasma membrane 1Q05CJ524
Grin2cglutamate receptor, ionotropic, NMDA2C (epsilon 3)Q010985
Grm3glutamate receptor, metabotropic 3Q9QYS22
Kcnj16potassium inwardly-rectifying channel, subfamily J, member 16Q9Z3077
Lrp1low density lipoprotein receptor-related protein 1Q91ZX723
Lrrtm1leucine rich repeat transmembrane neuronal 1Q8K3772
Slc1a3Sodium-dependent glutamate/aspartate transporter 1P5656433
Slc2a1solute carrier family 2 (facilitated glucose transporter), member 1P1780922
Vdac3voltage-dependent anion channel 3Q6093125
Cytoeskeletal/ Structural/ Cell adhesion
Mtap1amicrotubule-associated protein 1 AQ9QYR632
Nlgn2neuroligin 2Q69ZK92
Nlgn3neuroligin 3A2AGI22
Syn2synapsin IIQ6433234
Shank1SH3/ankyrin domain gene 1XP_0014749604
Enzymes
CitcitronP4902511
Crymcrystallin, muO5498333
Csmd2CUB and Sushi multiple domains 2A2A8D75
Dusp10dual specificity phosphatase 10Q8R3L332
Jak3Janus kinase 3Q621372
Mapk3mitogen activated protein kinase 3Q638444
Ube2v1ubiquitin-conjugating enzyme E2 variant 1Q9CZY325
Ube2v2ubiquitin-conjugating enzyme E2 variant 2Q9D2M824
G-protein signalling
Gna13guanine nucleotide binding protein, alpha 13Q8C5L222
Gnb1guanine nucleotide binding protein (G protein), beta 1P6287433
Rab6RAB6, member RAS oncogene familyP352794
Signalling
Fbxo2F-box protein 2Q80UW24
Fbxo6F-box protein 6Q9QZN434
Nxph3neurexophilin 3Q91VX52
Pcbp2poly(rC) binding protein 2Q6199033
Traf3Tnf receptor-associated factor 3Q3UHJ133
Chaperone/ Protein folding/ Signalling
Hspa12aheat shock protein 12AQ8K0U42
DNA binding
Hist1h2bj*histone 1, H2bbQ8CGP233
Transcription/Translation
Eef1a1*eukaryotic translation elongation factor 1 alpha 1P1012622
Lsm11U7 snRNP-specific Sm-like protein LSM11Q8BUV623
Unclassified
CluclusterinQ0689023
Lancl1LanC (bacterial lantibiotic synthetase component C)-like 1O891124
Mogmyelin oligodendrocyte glycoproteinQ6188522
Neto1neuropilin (NRP) and tolloid (TLL)-like 1Q8R4I722

+ indicates NCBI accession number.
Genes marked with an asterisk represent genes whose peptides are common to other genes:
Eif1a1*: Eif1a2. Hist1h2bj*: Hist1h2bm, Hist1h2be, Hist1h2bn, Hist1h2bg, Hist1h2bp, Hist1h2bh, Hist1h2bf, Hist1h2bb, Hist3h2bb, Hist1h2bc, Hist1h2bl, Hist2h2bb.