G2C::Proteomics

Targeted tandem affinity purification of PSD-95 recovers core
postsynaptic complexes and schizophrenia susceptibility proteins
Esperanza Fernandez, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama,
Mike DR Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary and Seth GN Grant


Supplementary Table 7. Gene targeting and genotyping primers.

Primers used for the gene targeting vector generation and genotyping screening.

PrimersSequence
XbaIHATF15'-CGGCGCTCTAGAAAGGATCATCTCATCCACAATGTCC
HATR15'TGCTGCTGCCGTTGGGAGCTCACCCCTGAAAATACAAATTCTCGATCTTGTTGTGGGGCATGAGCG
F1Flag5'-GAGAATTTGTATTTTCAGGGTGAGCTCCCAACGGCAGCAGACTACAAAGACCATGACGGTG
BclIFlagR15'-GTCGGTACTTATCGTCTCTTGTCATACACCGTTGATCAC
Psd95HAXhoIF5'-GCCACATCTGTAACTCCGCTCGAGGCCGTGAG
Psd95HAXbaIR5'CTGCTCTAGACCGAGTCTCTCTCGGGCTGGGA
Psd95HAAcc65IF5'-GCCTGGCTTGGCCTG'CTACGGGGTACCTTCCTGC
Psd95HABglIIR5'-GACCTAGATCTGCGGCCGCACGGGGGGACCGGTGGGAGGTGTGTGAAAG
Psd95HANdeIF5'-GGGTAATCTCCAGGCAAGAGTTTTATGCCCCACTTCCCCTTTCCT
Psd95HAPmlIR5'-GAGAGAGCCAGAGCCAGGCTCAGCCAAGTGCCGGCGTACAC
Psd95HAAscIF5'CATTTGGCGCGCCGGGGAAGTCGGACATGCCATCCAAC
Psd95HAPmeIR5'-CGTCACAAGCATCTGTAATCCTAGCTTTGTTTAAACCCCAGC5'
Psd95F55'-GGTCCCAGCCCGAGAGAGACTC
pneoR45'-CGTCCTGCACGACGCGAGCTGC
pneoF35'-GCCTCTGAGCTATTCCAGAAGTAG
Psd95R35'-CCTTGGTGTATGCACACATGC
Psd95R65'-GGCGGCAGCATTTCCTGTCCTC